PMID- 2721821
OWN - NLM
STAT- MEDLINE
DCOM- 19890623
LR  - 20131121
IS  - 0012-1630 (Print)
IS  - 0012-1630 (Linking)
VI  - 22
IP  - 4
DP  - 1989 May
TI  - Anterior and posterior, but not cheek, intraoral cannulation procedures elevate 
      serum corticosterone levels in neonatal rat pups.
PG  - 401-11
AB  - Implantation of intraoral cannulas is a procedure that has been typically assumed 
      to be relatively unstressful in neonatal rat pups. To test this assumption, 
      endocrine responses to such implantations were compared with those of other 
      standard procedures. In Experiment 1, corticosterone and growth hormone (GH) 
      levels were assessed in 4-day-old rat pups placed in an incubator for 15 or 60 
      min following either: no treatment, subcutaneous (sc) injection of 0.9% NaCl, 
      anterior or posterior intraoral cannulation, ice anesthesia or ether anesthesia. 
      Corticosterone levels were elevated relative to nontreated controls 15 min after 
      all treatments except sc injection. These levels remained elevated after 60 min 
      in both cannulation groups and the ice anesthesia group. In Experiment 2, the 
      ability of ether anesthesia to reduce the hormonal response to the cannulation 
      procedures was assessed in addition to examining the hormonal response to 
      intraoral cannulations through the cheek in 4-day-old rat pups. Ether did not 
      attenuate the corticosterone response to either anterior or posterior 
      cannulations. Pups subjected to the cheek cannulation procedure did not exhibit 
      any significant alterations in serum corticosterone levels when compared with 
      nontreated control pups. GH levels were found to differentiate less among the 
      various procedures than corticosterone levels, with GH levels generally being low 
      in all groups, including nontreated control animals. These data suggest that a 
      cheek placement is less stressful than anterior and posterior placements and may 
      provide a viable alternative in studies necessitating the implantation of cannula 
      into the buccal cavity during the early postnatal period.
FAU - Spear, L P
AU  - Spear LP
AD  - Department of Psychology, SUNY, Binghamton, 13901.
FAU - Specht, S M
AU  - Specht SM
FAU - Kirstein, C L
AU  - Kirstein CL
FAU - Kuhn, C M
AU  - Kuhn CM
LA  - eng
GR  - DA02739/DA/NIDA NIH HHS/United States
GR  - DA04478/DA/NIDA NIH HHS/United States
GR  - MH13699/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Dev Psychobiol
JT  - Developmental psychobiology
JID - 0164074
RN  - 9002-72-6 (Growth Hormone)
RN  - W980KJ009P (Corticosterone)
SB  - IM
MH  - Anesthesia, General
MH  - Animals
MH  - Animals, Newborn/*blood
MH  - Arousal/*physiology
MH  - Catheters, Indwelling
MH  - Cheek
MH  - Corticosterone/*blood
MH  - Female
MH  - Growth Hormone/blood
MH  - Male
MH  - Mouth
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Social Environment
EDAT- 1989/05/01 00:00
MHDA- 1989/05/01 00:01
CRDT- 1989/05/01 00:00
PHST- 1989/05/01 00:00 [pubmed]
PHST- 1989/05/01 00:01 [medline]
PHST- 1989/05/01 00:00 [entrez]
AID - 10.1002/dev.420220407 [doi]
PST - ppublish
SO  - Dev Psychobiol. 1989 May;22(4):401-11. doi: 10.1002/dev.420220407.