PMID- 27219292
OWN - NLM
STAT- MEDLINE
DCOM- 20170221
LR  - 20201209
IS  - 1879-1298 (Electronic)
IS  - 0045-6535 (Linking)
VI  - 157
DP  - 2016 Aug
TI  - Microcystin-LR induced developmental toxicity and apoptosis in zebrafish (Danio 
      rerio) larvae by activation of ER stress response.
PG  - 166-73
LID - S0045-6535(16)30678-6 [pii]
LID - 10.1016/j.chemosphere.2016.05.038 [doi]
AB  - Recent studies have demonstrated that cyanobacteria-derived Microcystin-LR 
      (MC-LR) can cause developmental toxicity and trigger apoptosis in zebrafish 
      (Danio rerio) larvae, but the underlying mechanisms remain largely unknown. In 
      this study, we tested the hypothesis that the mechanism by which MC-LR induces 
      developmental toxicity is through activation of endoplasmic reticulum (ER) 
      stress. MC-LR (4.0 muM) exposure through submersion caused serious developmental 
      toxicity, such as malformation, growth delay and decreased heart rates in 
      zebrafish larvae, which could be inhibited by ER stress blocker, 
      tauroursodeoxycholic acid (TUDCA, 20 muM). Meanwhile, acridine orange (AO) 
      staining showed TUDCA could rescue cell apoptosis in heart area in zebrafish 
      larvae resulted by MC-LR exposure. Real-time polymerase chain reaction (real-time 
      PCR) analysis demonstrated that MC-LR induced activation of ER stress which 
      consequently triggered apoptosis in zebrafish larvae. Protein expression examined 
      by western blot indicated that MC-LR could activate MAPK8/Bcl-2/Bax pathway and 
      caspase-dependent apoptotic pathway in zebrafish larva and the effects were 
      mitigated by inhibition of ER stress. Taken together, the results observed in 
      this study suggested that ER stress plays a critical role in developmental 
      toxicity and apoptosis in zebrafish embryos exposed to MC-LR.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Qi, Mei
AU  - Qi M
AD  - College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China.
FAU - Dang, Yao
AU  - Dang Y
AD  - College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China.
FAU - Xu, Qinglong
AU  - Xu Q
AD  - College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China.
FAU - Yu, Liqin
AU  - Yu L
AD  - College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China; 
      Hubei Provincial Engineering Laboratory for Pond Aquaculture, Wuhan 430070, 
      China.
FAU - Liu, Chunsheng
AU  - Liu C
AD  - College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China; 
      Hubei Provincial Engineering Laboratory for Pond Aquaculture, Wuhan 430070, 
      China; Collaborative Innovation Center for Efficient and Health Production of 
      Fisheries in Hunan Province, Changde 415000, China.
FAU - Yuan, Yongchao
AU  - Yuan Y
AD  - College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China.
FAU - Wang, Jianghua
AU  - Wang J
AD  - College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China; 
      Hubei Provincial Engineering Laboratory for Pond Aquaculture, Wuhan 430070, 
      China. Electronic address: whtjwjh@163.com.
LA  - eng
PT  - Journal Article
DEP - 20160521
PL  - England
TA  - Chemosphere
JT  - Chemosphere
JID - 0320657
RN  - 0 (Bacterial Toxins)
RN  - 0 (Marine Toxins)
RN  - 0 (Microcystins)
RN  - 0 (Water Pollutants, Chemical)
RN  - 516-35-8 (Taurochenodeoxycholic Acid)
RN  - 60EUX8MN5X (ursodoxicoltaurine)
RN  - EQ8332842Y (cyanoginosin LR)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Bacterial Toxins/toxicity
MH  - Dose-Response Relationship, Drug
MH  - Endoplasmic Reticulum Stress/*drug effects
MH  - Marine Toxins
MH  - Microcystins/*toxicity
MH  - Random Allocation
MH  - Taurochenodeoxycholic Acid/*pharmacology
MH  - Water Pollutants, Chemical/*toxicity
MH  - Zebrafish/growth & development/*metabolism
OTO - NOTNLM
OT  - Developmental toxicity
OT  - Endoplasmic reticulum
OT  - Microcystin
OT  - Stress
OT  - Zebrafish
EDAT- 2016/05/25 06:00
MHDA- 2017/02/22 06:00
CRDT- 2016/05/25 06:00
PHST- 2016/03/29 00:00 [received]
PHST- 2016/05/12 00:00 [revised]
PHST- 2016/05/12 00:00 [accepted]
PHST- 2016/05/25 06:00 [entrez]
PHST- 2016/05/25 06:00 [pubmed]
PHST- 2017/02/22 06:00 [medline]
AID - S0045-6535(16)30678-6 [pii]
AID - 10.1016/j.chemosphere.2016.05.038 [doi]
PST - ppublish
SO  - Chemosphere. 2016 Aug;157:166-73. doi: 10.1016/j.chemosphere.2016.05.038. Epub 
      2016 May 21.