PMID- 27221552
OWN - NLM
STAT- MEDLINE
DCOM- 20180402
LR  - 20240325
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 6
DP  - 2016 May 25
TI  - Hyperhomocysteinaemia in rats is associated with erectile dysfunction by 
      impairing endothelial nitric oxide synthase activity.
PG  - 26647
LID - 10.1038/srep26647 [doi]
LID - 26647
AB  - To investigate the effect of hyperhomocysteinaemia (HHCy) on penile erectile 
      function in a rat model, a methionine-rich diet was used in which erectile 
      function, the reproductive system, and nitric oxide synthase were characterized. 
      The intracavernous pressure, apomorphine experiments, measurement of oxidative 
      stress, hematoxylin and eosin staining, immunohistochemistry analysis, reverse 
      transcription-polymerase chain reactions and measurement of endothelial nitric 
      oxide synthase activity were utilized. Our results showed that erections in the 
      middle-dose, high-dose, and interference (INF) groups were significantly lower 
      than the control (P < 0.05). INF group, being fed with vitamins B and folic acid, 
      demonstrated markedly improved penile erections compared with the middle-dose 
      group (P < 0.05). HHCy-induced eNOS and phospho-eNOS protein expression was 
      reduced and the antioxidant effect was markedly impaired. The data of the present 
      data provide evidence that HHCy is a vascular risk factor for erectile 
      dysfunction by impairing cavernosa endothelial nitric oxide synthase activity. 
      Intake of vitamins B can alleviate this abnormality.
FAU - Jiang, Weijun
AU  - Jiang W
AD  - Department of Reproduction and Genetics, Institute of Laboratory Medicine, 
      Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, P.R. 
      China.
FAU - Xiong, Lei
AU  - Xiong L
AD  - Department of Cardiothoracic surgery, Jinling Hospital, Nanjing University School 
      of Medicine, Nanjing 210002, P.R. China.
FAU - Bin Yang
AU  - Bin Yang
AD  - Department of Reproduction and Genetics, Institute of Laboratory Medicine, 
      Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, P.R. 
      China.
FAU - Li, Weiwei
AU  - Li W
AD  - Department of Reproduction and Genetics, Institute of Laboratory Medicine, 
      Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, P.R. 
      China.
FAU - Zhang, Jing
AU  - Zhang J
AD  - Department of Reproduction and Genetics, Institute of Laboratory Medicine, 
      Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, P.R. 
      China.
FAU - Zhou, Qing
AU  - Zhou Q
AD  - Department of Reproduction and Genetics, Institute of Laboratory Medicine, 
      Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, P.R. 
      China.
FAU - Wu, Qiuyue
AU  - Wu Q
AD  - Department of Reproduction and Genetics, Institute of Laboratory Medicine, 
      Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, P.R. 
      China.
FAU - Li, Tianfu
AU  - Li T
AD  - Department of Reproduction and Genetics, Institute of Laboratory Medicine, 
      Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, P.R. 
      China.
FAU - Zhang, Cui
AU  - Zhang C
AD  - Department of Reproduction and Genetics, Institute of Laboratory Medicine, 
      Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, P.R. 
      China.
FAU - Zhang, Mingchao
AU  - Zhang M
AD  - Department of Reproduction and Genetics, Institute of Laboratory Medicine, 
      Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, P.R. 
      China.
FAU - Xia, Xinyi
AU  - Xia X
AD  - Department of Reproduction and Genetics, Institute of Laboratory Medicine, 
      Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, P.R. 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160525
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type III)
RN  - EC 1.14.13.39 (Nos3 protein, rat)
SB  - IM
MH  - Animals
MH  - Erectile Dysfunction/chemically induced/*enzymology
MH  - Hyperhomocysteinemia/chemically induced/*enzymology
MH  - Male
MH  - Nitric Oxide Synthase Type III/*biosynthesis
MH  - Rats
MH  - Rats, Sprague-Dawley
PMC - PMC4879575
EDAT- 2016/05/26 06:00
MHDA- 2018/04/03 06:00
PMCR- 2016/05/25
CRDT- 2016/05/26 06:00
PHST- 2015/07/27 00:00 [received]
PHST- 2016/05/06 00:00 [accepted]
PHST- 2016/05/26 06:00 [entrez]
PHST- 2016/05/26 06:00 [pubmed]
PHST- 2018/04/03 06:00 [medline]
PHST- 2016/05/25 00:00 [pmc-release]
AID - srep26647 [pii]
AID - 10.1038/srep26647 [doi]
PST - epublish
SO  - Sci Rep. 2016 May 25;6:26647. doi: 10.1038/srep26647.