PMID- 27222146
OWN - NLM
STAT- MEDLINE
DCOM- 20170306
LR  - 20170306
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Linking)
VI  - 38
IP  - 1
DP  - 2016 Jul
TI  - RbAp48, a novel inhibitory factor that regulates the transcription of human 
      immunodeficiency virus type 1.
PG  - 267-74
LID - 10.3892/ijmm.2016.2598 [doi]
AB  - Retinoblastoma binding protein 4 (RbAp48) is a histone chaperone which has been 
      suggested to play a role in gene silencing. However, the role of RbAp48 in human 
      immunodeficiency virus type 1 (HIV-1) infection and gene replication has not been 
      determined to date, to the best of our knowledge. For this purpose, we 
      demonstrated in the present study that RbAp48 expression was upregulated by HIV-1 
      infection, whereas the knockdown of RbAp48 promoted HIV infection and the 
      production of virus particles. The ectopic expression of RbAp48 inhibited HIV-1 
      expression, and this inhibition correlated with a marked decrease in the 
      expression of HIV-1 genomic RNA and various RNA transcripts. Further experiments 
      to determine the mechanism responsible for the inhibitory effects of RbAp48 
      revealed that the ectopic expression of RbAp48 repressed HIV-1 long terminal 
      repeat (LTR)-mediated basal transcription as well as TNF-alpha- and phorbol 
      12-myristate 13-acetate (PMA)‑activated transcription. Furthermore, the results 
      of the electrophoretic mobility shift assay (EMSA) and chromatin 
      immunoprecipitation (ChIP) analysis revealed that RbAp48 binds to the HIV-1 LTR 
      in vitro. Taken together, these findings demonstrate that, as a transcriptional 
      cofactor, RbAp48 is likely to act as a potent antiretroviral defense.
FAU - Wang, Juan
AU  - Wang J
AD  - State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The 
      First Af fi liated Hospital, College of Medicine, Zhejiang University, Hangzhou, 
      Zhejiang 310003, P.R. China.
FAU - Yang, Jin
AU  - Yang J
AD  - Department of Medicine, Blood Center of Zhejiang Province, Hangzhou, Zhejiang 
      330100, P.R. China.
FAU - Yang, Zongxing
AU  - Yang Z
AD  - Xixi Hospital of Hangzhou, Hangzhou, Zhejiang 310023, P.R. China.
FAU - Lu, Xiangyun
AU  - Lu X
AD  - State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The 
      First Af fi liated Hospital, College of Medicine, Zhejiang University, Hangzhou, 
      Zhejiang 310003, P.R. China.
FAU - Jin, Changzhong
AU  - Jin C
AD  - State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The 
      First Af fi liated Hospital, College of Medicine, Zhejiang University, Hangzhou, 
      Zhejiang 310003, P.R. China.
FAU - Cheng, Linfang
AU  - Cheng L
AD  - State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The 
      First Af fi liated Hospital, College of Medicine, Zhejiang University, Hangzhou, 
      Zhejiang 310003, P.R. China.
FAU - Wu, Nanping
AU  - Wu N
AD  - State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The 
      First Af fi liated Hospital, College of Medicine, Zhejiang University, Hangzhou, 
      Zhejiang 310003, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20160519
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (Retinoblastoma-Binding Protein 4)
SB  - IM
MH  - HEK293 Cells
MH  - HIV Infections/genetics/virology
MH  - HIV Long Terminal Repeat/genetics
MH  - HIV-1/*genetics
MH  - Humans
MH  - Intracellular Space/metabolism
MH  - Promoter Regions, Genetic/genetics
MH  - Protein Binding
MH  - Retinoblastoma-Binding Protein 4/*metabolism
MH  - *Transcription, Genetic
EDAT- 2016/05/26 06:00
MHDA- 2017/03/07 06:00
CRDT- 2016/05/26 06:00
PHST- 2015/10/31 00:00 [received]
PHST- 2016/05/10 00:00 [accepted]
PHST- 2016/05/26 06:00 [entrez]
PHST- 2016/05/26 06:00 [pubmed]
PHST- 2017/03/07 06:00 [medline]
AID - 10.3892/ijmm.2016.2598 [doi]
PST - ppublish
SO  - Int J Mol Med. 2016 Jul;38(1):267-74. doi: 10.3892/ijmm.2016.2598. Epub 2016 May 
      19.