PMID- 27222722
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160525
LR  - 20220331
IS  - 2662-6810 (Print)
IS  - 2052-7276 (Electronic)
IS  - 2052-7276 (Linking)
VI  - 3
DP  - 2016
TI  - A survey of FLS2 genes from multiple citrus species identifies candidates for 
      enhancing disease resistance to Xanthomonas citri ssp. citri.
PG  - 16022
LID - 10.1038/hortres.2016.22 [doi]
AB  - Pathogen-associated molecular patterns (PAMPs)-triggered immunity (PTI) is an 
      important component of plant innate immunity. In a previous study, we showed that 
      the PAMP flg22 from Xanthomonas citri ssp. citri (Xflg22), the causal agent of 
      citrus canker, induced PTI in citrus, which correlated with the observed levels 
      of canker resistance. Here, we identified and sequenced two bacterial 
      flagellin/flg22 receptors (FLS2-1 and FLS2-2) from 'Duncan' grapefruit (Citrus 
      paradisi, CpFLS2-1 and CpFLS2-2) and 'Sun Chu Sha' mandarin (C. reticulata, 
      CrFLS2-1 and CrFLS2-2). We were able to isolate only one FLS2 from 'Nagami' 
      kumquat (Fortunella margarita, FmFLS2-1) and gene flanking sequences suggest a 
      rearrangement event that resulted in the deletion of FLS2-2 from the genome. 
      Phylogenetic analysis, gene structure and presence of critical amino acid domains 
      all indicate we identified the true FLS2 genes in citrus. FLS2-2 was more 
      transcriptionally responsive to Xflg22 than FLS2-1, with induced expression 
      levels higher in canker-resistant citrus than in susceptible ones. Interestingly, 
      'Nagami' kumquat showed the highest FLS2-1 steady-state expression levels, 
      although it was not induced by Xflg22. We selected FmFLS2-1, CrFLS2-2 and 
      CpFLS2-2 to further evaluate their capacity to enhance bacterial resistance using 
      Agrobacterium-mediated transient expression assays. Both FmFLS2-1 and CrFLS2-2, 
      the two proteins from canker-resistant species, conferred stronger Xflg22 
      responses and reduced canker symptoms in leaves of the susceptible grapefruit 
      genotype. These two citrus genes will be useful resources to enhance PTI and 
      achieve resistance against canker and possibly other bacterial pathogens in 
      susceptible citrus types.
FAU - Shi, Qingchun
AU  - Shi Q
AUID- ORCID: 0000-0002-0333-9451
AD  - Horticultural Sciences Department, University of Florida , Gainesville, FL 32611, 
      USA.
FAU - Febres, Vicente J
AU  - Febres VJ
AD  - Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, 
      USA; Plant Molecular and Cellular Biology Program, University of Florida, 
      Gainesville, FL 32611, USA.
FAU - Jones, Jeffrey B
AU  - Jones JB
AD  - Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, 
      FL 32611, USA; Plant Pathology Department, University of Florida, Gainesville, FL 
      32611, USA.
FAU - Moore, Gloria A
AU  - Moore GA
AD  - Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, 
      USA; Plant Molecular and Cellular Biology Program, University of Florida, 
      Gainesville, FL 32611, USA.
LA  - eng
PT  - Journal Article
DEP - 20160511
PL  - England
TA  - Hortic Res
JT  - Horticulture research
JID - 101655540
PMC - PMC4863249
EDAT- 2016/05/26 06:00
MHDA- 2016/05/26 06:01
PMCR- 2016/05/11
CRDT- 2016/05/26 06:00
PHST- 2016/03/03 00:00 [received]
PHST- 2016/04/03 00:00 [revised]
PHST- 2016/04/05 00:00 [accepted]
PHST- 2016/05/26 06:00 [entrez]
PHST- 2016/05/26 06:00 [pubmed]
PHST- 2016/05/26 06:01 [medline]
PHST- 2016/05/11 00:00 [pmc-release]
AID - hortres201622 [pii]
AID - 10.1038/hortres.2016.22 [doi]
PST - epublish
SO  - Hortic Res. 2016 May 11;3:16022. doi: 10.1038/hortres.2016.22. eCollection 2016.