PMID- 27224314
OWN - NLM
STAT- MEDLINE
DCOM- 20170706
LR  - 20190213
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 11
IP  - 5
DP  - 2016
TI  - Comparison between DNA Detection in Trigeminal Nerve Ganglia and Serology to 
      Detect Cattle Infected with Bovine Herpesviruses Types 1 and 5.
PG  - e0155941
LID - 10.1371/journal.pone.0155941 [doi]
LID - e0155941
AB  - Bovine herpesviruses (BoHVs) types 1 (BoHV-1) and 5 (BoHV-5) are 
      alphaherpesviruses of major importance to the bovine production chain. Such 
      viruses are capable of establishing latent infections in neuronal tissues. 
      Infected animals tend to develop a serological response to infection; however, 
      such response-usually investigated by antibody assays in serum-may eventually not 
      be detected in laboratory assays. Nevertheless, serological tests such as virus 
      neutralization (VN) and various enzyme-linked immunosorbent assays (ELISAs) are 
      widely employed to check individual or herd status of BoHV infections. The 
      correlation between detection of antibodies and the presence of viral nucleic 
      acids as indicatives of infection in infected cattle has not been deeply 
      examined. In order to investigate such correlation, 248 bovine serum samples were 
      tested by VN to BoHV-1 and BoHV-5, as well as in a widely employed (though not 
      type-differential) gB ELISA (IDEXX IBR gB X2 Ab Test) in search for antibodies to 
      BoHVs. Immediately after blood withdrawal, cattle were slaughtered and trigeminal 
      ganglia (TG) excised for DNA extraction and viral nucleic acid detection (NAD) by 
      nested PCR. Neutralizing antibodies to BoHV-1 and/or BoHV-5 were detected in 
      44.8% (111/248) of sera, whereas the gB ELISA detected antibodies in 51.2% 
      (127/248) of the samples. However, genomes of either BoHV-1, BoHV-5, or both, 
      were detected in TGs of 85.9% (213/248) of the animals. These findings reveal 
      that the assays designed to detect antibodies to BoHV-1 and/or BoHV-5 employed 
      here may fail to detect a significant number of latently infected animals (in 
      this study, 35.7%). From such data, it is clear that antibody assays are poorly 
      correlated with detection of viral genomes in BoHV-1 and BoHV-5-infected animals.
FAU - Puentes, Rodrigo
AU  - Puentes R
AD  - Departamento de Ciencias Microbiologicas, Area de Inmunologia, Facultad de 
      Veterinaria, Universidad de la Republica Oriental del Uruguay (UdelaR), 
      Montevideo, Uruguay.
FAU - Campos, Fabricio Souza
AU  - Campos FS
AD  - Laboratorio de Microbiologia Veterinaria, Faculdade de Agronomia e Medicina 
      Veterinaria, Universidade de Brasilia (UnB), Distrito Federal (DF), Brazil.
FAU - Furtado, Agustin
AU  - Furtado A
AD  - Departamento de Ciencias Microbiologicas, Area de Inmunologia, Facultad de 
      Veterinaria, Universidad de la Republica Oriental del Uruguay (UdelaR), 
      Montevideo, Uruguay.
FAU - Torres, Fabricio Dias
AU  - Torres FD
AD  - Laboratorio de Virologia, Departamento de Microbiologia, Imunologia e 
      Parasitologia, Instituto de Ciencias Basicas da Saude, Universidade Federal do 
      Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.
FAU - Franco, Ana Claudia
AU  - Franco AC
AD  - Laboratorio de Virologia, Departamento de Microbiologia, Imunologia e 
      Parasitologia, Instituto de Ciencias Basicas da Saude, Universidade Federal do 
      Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.
FAU - Maisonnave, Jacqueline
AU  - Maisonnave J
AD  - Departamento de Ciencias Microbiologicas, Area de Inmunologia, Facultad de 
      Veterinaria, Universidad de la Republica Oriental del Uruguay (UdelaR), 
      Montevideo, Uruguay.
FAU - Roehe, Paulo Michel
AU  - Roehe PM
AD  - Laboratorio de Virologia, Departamento de Microbiologia, Imunologia e 
      Parasitologia, Instituto de Ciencias Basicas da Saude, Universidade Federal do 
      Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160525
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Antibodies, Viral)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/*immunology
MH  - Cattle
MH  - *Cattle Diseases/diagnosis/genetics/immunology
MH  - Cell Line
MH  - DNA, Viral/*genetics
MH  - *Encephalitis, Viral/diagnosis/genetics/immunology/veterinary
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - *Herpesviridae Infections/diagnosis/genetics/immunology/veterinary
MH  - *Herpesvirus 1, Bovine/genetics/immunology
MH  - *Herpesvirus 5, Bovine/genetics/immunology
MH  - *Meningoencephalitis/diagnosis/genetics/immunology/veterinary
MH  - Polymerase Chain Reaction/methods
MH  - Trigeminal Ganglion/*virology
PMC - PMC4880179
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2016/05/26 06:00
MHDA- 2017/07/07 06:00
PMCR- 2016/05/25
CRDT- 2016/05/26 06:00
PHST- 2016/01/06 00:00 [received]
PHST- 2016/05/08 00:00 [accepted]
PHST- 2016/05/26 06:00 [entrez]
PHST- 2016/05/26 06:00 [pubmed]
PHST- 2017/07/07 06:00 [medline]
PHST- 2016/05/25 00:00 [pmc-release]
AID - PONE-D-16-00648 [pii]
AID - 10.1371/journal.pone.0155941 [doi]
PST - epublish
SO  - PLoS One. 2016 May 25;11(5):e0155941. doi: 10.1371/journal.pone.0155941. 
      eCollection 2016.