PMID- 27224853
OWN - NLM
STAT- MEDLINE
DCOM- 20180730
LR  - 20181202
IS  - 1936-086X (Electronic)
IS  - 1936-0851 (Print)
IS  - 1936-0851 (Linking)
VI  - 10
IP  - 6
DP  - 2016 Jun 28
TI  - Conformal Nanoencapsulation of Allogeneic T Cells Mitigates Graft-versus-Host 
      Disease and Retains Graft-versus-Leukemia Activity.
PG  - 6189-200
LID - 10.1021/acsnano.6b02206 [doi]
AB  - Allogeneic transplantation of hematopoietic stem cells (HSC) in combination with 
      T cells has a curative potential for hematopoietic malignancies through 
      graft-versus-leukemia (GVL) effects, but is often compromised by the notorious 
      side effect of graft-versus-host disease (GVHD) resulting from alloreactivity of 
      the donor T cells. Here, we tested if temporary immunoisolation achieved by 
      conformally encapsulating the donor T cells within a biocompatible and 
      biodegradable porous film ( approximately 450 nm in thickness) of chitosan and alginate could 
      attenuate GVHD without compromising GVL. The nanoencapsulation was found not to 
      affect the phenotype of T cells in vitro in terms of size, viability, 
      proliferation, cytokine secretion, and cytotoxicity against tumor cells. 
      Moreover, the porous nature of the nanoscale film allowed the encapsulated T 
      cells to communicate with their environment, as evidenced by their intact 
      capability of binding to antibodies. Lethally irradiated mice transplanted with 
      bone marrow cells (BMCs) and the conformally encapsulated allogeneic T cells 
      exhibited significantly improved survival and reduced GVHD together with minimal 
      liver damage and enhanced engraftment of donor BMCs, compared to the 
      transplantation of BMCs and non-encapsulated allogeneic T cells. Moreover, the 
      conformal nanoencapsulation did not compromise the GVL effect of the donor T 
      cells. These data show that conformal nanoencapsulation of T cells within 
      biocompatible and biodegradable nanoscale porous materials is a potentially safe 
      and effective approach to improve allogeneic HSC transplantation for treating 
      hematological malignancies and possibly other diseases.
FAU - Zhao, Shuting
AU  - Zhao S
FAU - Zhang, Lingling
AU  - Zhang L
AD  - Institute of Clinical Pharmacology, Anhui Medical University , Hefei 230032, 
      China.
FAU - Han, Jianfeng
AU  - Han J
FAU - Chu, Jianhong
AU  - Chu J
AD  - Suzhou Institute of Blood and Marrow Transplantation, Soochow University , Suzhou 
      215000, China.
FAU - Wang, Hai
AU  - Wang H
FAU - Chen, Xilin
AU  - Chen X
FAU - Wang, Youwei
AU  - Wang Y
FAU - Tun, Norm
AU  - Tun N
FAU - Lu, Lanchun
AU  - Lu L
FAU - Bai, Xue-Feng
AU  - Bai XF
FAU - Yearsley, Martha
AU  - Yearsley M
FAU - Devine, Steven
AU  - Devine S
FAU - He, Xiaoming
AU  - He X
FAU - Yu, Jianhua
AU  - Yu J
LA  - eng
GR  - R01 AI123661/AI/NIAID NIH HHS/United States
GR  - R01 CA068458/CA/NCI NIH HHS/United States
GR  - R01 CA155521/CA/NCI NIH HHS/United States
GR  - R01 CA185301/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20160531
PL  - United States
TA  - ACS Nano
JT  - ACS nano
JID - 101313589
SB  - IM
MH  - Animals
MH  - *Graft vs Host Disease
MH  - *Graft vs Leukemia Effect
MH  - Leukemia
MH  - Mice
MH  - *Nanocomposites
MH  - *T-Lymphocytes
MH  - Transplantation, Homologous
PMC - PMC5514314
MID - NIHMS875671
OTO - NOTNLM
OT  - T cells
OT  - alginate
OT  - chitosan
OT  - graft-versus-host disease
OT  - graft-versus-leukemia
OT  - immunoisolation
OT  - nanomaterials
COIS- Notes The authors declare the following competing financial interest(s): SZ and 
      XH disclosed the technology of conformal nanoencapsulation of cells and tissues 
      to the Technology and Commercialization Office at The Ohio State University, 
      Columbus, Ohio, USA.
EDAT- 2016/05/26 06:00
MHDA- 2018/07/31 06:00
PMCR- 2017/07/18
CRDT- 2016/05/26 06:00
PHST- 2016/05/26 06:00 [entrez]
PHST- 2016/05/26 06:00 [pubmed]
PHST- 2018/07/31 06:00 [medline]
PHST- 2017/07/18 00:00 [pmc-release]
AID - 10.1021/acsnano.6b02206 [doi]
PST - ppublish
SO  - ACS Nano. 2016 Jun 28;10(6):6189-200. doi: 10.1021/acsnano.6b02206. Epub 2016 May 
      31.