PMID- 27225204
OWN - NLM
STAT- MEDLINE
DCOM- 20160822
LR  - 20160526
IS  - 0177-5103 (Print)
IS  - 0177-5103 (Linking)
VI  - 119
IP  - 3
DP  - 2016 May 26
TI  - Characterization and identification of streptococci from golden pompano in China.
PG  - 207-17
LID - 10.3354/dao02998 [doi]
AB  - Streptococcal infections cause significant mortality and high economic losses in 
      the fish farm industry worldwide, including in the culture of golden pompano 
      Trachinotus ovatus L., a species gaining popularity in China. A total of 9 
      streptococcal strains were isolated from cage-cultured diseased golden pompano in 
      Beihai, Zhanjing, and Shenzhen, China, between 2012 and 2014. Conventional and 
      rapid identification systems were used to determine that the isolates were 
      Streptococcus agalactiae, S. iniae, and S. dysgalactiae subsp. dysgalactiae. All 
      isolates were gram-positive cocci cells in pairs or short-chain, non-motile, 
      catalase negative, alpha or beta hemolytic cocci. The results of multiplex PCR assays 
      and 16S rRNA BLAST analysis also showed that the beta hemolytic strains were S. 
      agalactiae and S. iniae and the alpha hemolytic strain was S. dysgalactiae subsp. 
      dysgalactiae, respectively. Pathogenicity assays revealed that S. agalactiae 
      (lethal dose [LD50]: 6.38 x 10(4) CFU ml(-1)) was more virulent for golden 
      pompano than S. iniae (LD50: 1.47 x 10(7) CFU ml(-1)) and S. dysgalactiae subsp. 
      dysgalactiae (LD50: 2.57 x 10(6) CFU ml(-1)) when they were challenged by 
      intraperiotoneal (i.p.) injection. The results of antibiotic susceptibility 
      showed that all strains were extremely susceptible to cefradine, erythromycin, 
      and cefotaxime but resistant to gentamicin, penicillin G, novobiocin, neomycin, 
      ciprofloxacin, roxithromycin, furazolidone, enrofloxacin, norfloxacin, kanamycin, 
      ampicillin, tetracycline, and vancomycin This is the first report of a phenomenon 
      of golden pompano coinfection with S. agalactiae and S. iniae, which will 
      contribute to the diagnosis and prevention of streptococcicosis.
FAU - Cai, X H
AU  - Cai XH
AD  - Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for 
      Aquatic Economic Animals, Guangdong Key Laboratory of Control for Diseases of 
      Aquatic Economic Animals, Guangdong Ocean University, Zhanjiang 524088, PR China.
FAU - Peng, Y H
AU  - Peng YH
FAU - Wang, Z C
AU  - Wang ZC
FAU - Huang, T
AU  - Huang T
FAU - Xiong, X Y
AU  - Xiong XY
FAU - Huang, Y C
AU  - Huang YC
FAU - Wang, B
AU  - Wang B
FAU - Xu, L W
AU  - Xu LW
FAU - Wu, Z H
AU  - Wu ZH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Dis Aquat Organ
JT  - Diseases of aquatic organisms
JID - 8807037
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/pharmacology
MH  - Aquaculture
MH  - China/epidemiology
MH  - Drug Resistance, Bacterial
MH  - Fish Diseases/*microbiology
MH  - Fishes
MH  - Phylogeny
MH  - RNA, Bacterial/genetics
MH  - RNA, Ribosomal, 16S/genetics
MH  - Streptococcal Infections/epidemiology/microbiology/*veterinary
MH  - Streptococcus/genetics/*isolation & purification/pathogenicity
EDAT- 2016/05/27 06:00
MHDA- 2016/08/23 06:00
CRDT- 2016/05/27 06:00
PHST- 2016/05/27 06:00 [entrez]
PHST- 2016/05/27 06:00 [pubmed]
PHST- 2016/08/23 06:00 [medline]
AID - 10.3354/dao02998 [doi]
PST - ppublish
SO  - Dis Aquat Organ. 2016 May 26;119(3):207-17. doi: 10.3354/dao02998.