PMID- 27225283
OWN - NLM
STAT- MEDLINE
DCOM- 20170223
LR  - 20201209
IS  - 1434-4726 (Electronic)
IS  - 0937-4477 (Linking)
VI  - 273
IP  - 10
DP  - 2016 Oct
TI  - UreA and cagA genes of Helicobacter pylori in Egyptian patients with laryngeal 
      squamous cell carcinoma and benign laryngeal polyps: a cohort study.
PG  - 3243-8
LID - 10.1007/s00405-016-4114-2 [doi]
AB  - This work aims to estimate the prevalence of Helicobacter pylori ureA gene and 
      evaluate cagA gene-positive strains in both patients of laryngeal squamous cell 
      carcinoma (LSCC) and those with benign laryngeal polyps. This study included 49 
      patients confirmed pathologically to have LSCC and 15 patients with benign 
      laryngeal polyps over a period from June 2013 to March 2015. Samples of laryngeal 
      tissue were collected during direct laryngoscope under general anesthesia to be 
      pathologically evaluated followed by analysis for H. pylori detection. Each 
      laryngeal tissue sample was divided into three parts; one for bacteriological 
      examination, the second for pathological examination and the third for PCR to 
      detect both ureA and cagA genes. Out of 49 LSCC samples, 31 (64.6 %) was positive 
      for ureA by PCR. Out of them, 29 samples (93.5 %) were cagA positive. Only three 
      cases (20 %) of the benign laryngeal polyp were ureA positive by PCR and one of 
      them was cagA positive by PCR. By the bacteriological culture, only eight samples 
      (25.8 %) gave growth. All of them were ureA positive and only seven of them were 
      cagA positive. There was a significant association between presence of H. pylori 
      and LSCC as compared to benign laryngeal polyp which may contribute in the 
      pathogenesis of laryngeal carcinoma. These results should be confirmed by further 
      studies over larger number of cases.
FAU - Barakat, Ghada
AU  - Barakat G
AD  - Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura 
      University, Mansoura, Egypt. Barakat.ghada@yahoo.com.
FAU - Nabiel, Yasmin
AU  - Nabiel Y
AD  - Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura 
      University, Mansoura, Egypt.
FAU - Ali, Omima
AU  - Ali O
AD  - Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura 
      University, Mansoura, Egypt.
FAU - El-Nady, Ghada
AU  - El-Nady G
AD  - Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura 
      University, Mansoura, Egypt.
FAU - Musaad, Ahmed
AU  - Musaad A
AD  - Otorhinolarngology Department, Faculty of Medicine, Mansoura University, 
      Mansoura, Egypt.
FAU - El-Sharkawy, Asser
AU  - El-Sharkawy A
AD  - Otorhinolarngology Department, Faculty of Medicine, Mansoura University, 
      Mansoura, Egypt.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Observational Study
DEP - 20160525
PL  - Germany
TA  - Eur Arch Otorhinolaryngol
JT  - European archives of oto-rhino-laryngology : official journal of the European 
      Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the 
      German Society for Oto-Rhino-Laryngology - Head and Neck Surgery
JID - 9002937
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Bacterial Proteins)
RN  - 0 (cagA protein, Helicobacter pylori)
RN  - EC 3.5.1.5 (Urease)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Antigens, Bacterial/*genetics
MH  - Bacterial Proteins/*genetics
MH  - Carcinoma, Squamous Cell/*microbiology
MH  - Cohort Studies
MH  - Egypt
MH  - Gene Expression
MH  - Helicobacter pylori/genetics
MH  - Humans
MH  - Laryngeal Neoplasms/*microbiology
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Polyps/*microbiology
MH  - Urease/*genetics
OTO - NOTNLM
OT  - *Carcinoma
OT  - *Helicobacter pylori
OT  - *Laryngeal polyp
OT  - *PCR
EDAT- 2016/05/27 06:00
MHDA- 2017/02/24 06:00
CRDT- 2016/05/27 06:00
PHST- 2016/01/11 00:00 [received]
PHST- 2016/05/20 00:00 [accepted]
PHST- 2016/05/27 06:00 [entrez]
PHST- 2016/05/27 06:00 [pubmed]
PHST- 2017/02/24 06:00 [medline]
AID - 10.1007/s00405-016-4114-2 [pii]
AID - 10.1007/s00405-016-4114-2 [doi]
PST - ppublish
SO  - Eur Arch Otorhinolaryngol. 2016 Oct;273(10):3243-8. doi: 
      10.1007/s00405-016-4114-2. Epub 2016 May 25.