PMID- 27225448
OWN - NLM
STAT- MEDLINE
DCOM- 20170601
LR  - 20170601
IS  - 1875-5992 (Electronic)
IS  - 1871-5206 (Linking)
VI  - 17
IP  - 1
DP  - 2017
TI  - Cordycepin Affects Multiple Apoptotic Pathways to Mediate Hepatocellular 
      Carcinoma Cell Death.
PG  - 143-149
AB  - BACKGROUND: Cordycepin possesses anti-inflammatory, anti-metastatic and 
      anti-tumor properties. OBJECTIVE: The present study investigates the 
      anti-hepatocellular carcinoma activities of cordycepin in in vitro and in vivo 
      models. METHOD: Cell viability, apoptosis rate, intracellular reactive oxygen 
      species (ROS) level and mitochondrial membrane potential (MMP) were determined by 
      3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide bromide assay, 
      annexin V/propidium iodide double staining, 2',7'-dichlorfluorescein-diacetate 
      and 5,5',6,6'-tetrachloro-1,1',3,3' tetraethylbenzimidazolylcarbocyanine iodide 
      (JC-1) staining respectively. The expressions of pro-apoptosis and antiapoptosis 
      proteins were detected by western blot. A PLC/PRL/5-xenografted nude mouse model 
      was applied to further confirm the anti-tumor activities of cordycepin. RESULTS: 
      Cordycepin suppressed cell viability, enhanced apoptotic rate, inhibited cell 
      proliferation and increased cleaved poly (ADP-ribose) polymerase (PARP) level. 
      Apoptotic alteration on mitochondria and abnormal changes on b-cell lymphoma 2 
      (Bcl-2) and b-cell lymphoma-extra large (Bcl-xL) levels were observed in 
      cordycepin-treated cells. Furthermore, cordycepin suppressed the activation of 
      extracellular signaling-regulated kinase (ERKs) and mammalian target of rapamycin 
      (mTOR) in both PLC/PRF/5 and HepG2 cells. Finally, PLC/PRL/5-xengrafted BALB/c 
      athymic nude mice were performed to confirm cordycepin's anti-tumor action. 
      CONCLUSION: Our finding suggests that the anti-hepatocellular carcinoma 
      properties of cordycepin are related to its modulation of multiple anti-apoptotic 
      and pro-apoptotic pathways. Our study provides an experimental evidence for 
      cordycepin as a rational agent for hepatocellular carcinoma treatment.
FAU - Zhou, Yulin
AU  - Zhou Y
FAU - Guo, Zhihua
AU  - Guo Z
FAU - Meng, Qingfan
AU  - Meng Q
FAU - Lu, Jiahui
AU  - Lu J
FAU - Wang, Ning
AU  - Wang N
FAU - Liu, Hui
AU  - Liu H
FAU - Liang, Qiming
AU  - Liang Q
FAU - Quan, Yutong
AU  - Quan Y
FAU - Wang, Di
AU  - Wang D
AD  - School of Life Sciences, Jilin University, Changchun City, Jilin Province, PR 
      China. China.
FAU - Xie, Jing
AU  - Xie J
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Anticancer Agents Med Chem
JT  - Anti-cancer agents in medicinal chemistry
JID - 101265649
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Deoxyadenosines)
RN  - 0 (Reactive Oxygen Species)
RN  - GZ8VF4M2J8 (cordycepin)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents/pharmacology/*therapeutic use
MH  - Apoptosis/*drug effects
MH  - Carcinoma, Hepatocellular/*drug therapy/metabolism/pathology
MH  - Cell Survival/drug effects
MH  - Deoxyadenosines/pharmacology/*therapeutic use
MH  - Hep G2 Cells
MH  - Humans
MH  - Liver/drug effects/metabolism/pathology
MH  - Liver Neoplasms/*drug therapy/metabolism/pathology
MH  - Male
MH  - Membrane Potential, Mitochondrial/drug effects
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - Mitochondria/drug effects/metabolism/pathology
MH  - Reactive Oxygen Species/metabolism
MH  - Signal Transduction/drug effects
EDAT- 2016/05/27 06:00
MHDA- 2017/06/02 06:00
CRDT- 2016/05/27 06:00
PHST- 2016/02/02 00:00 [received]
PHST- 2016/03/26 00:00 [revised]
PHST- 2016/05/25 00:00 [accepted]
PHST- 2016/05/27 06:00 [pubmed]
PHST- 2017/06/02 06:00 [medline]
PHST- 2016/05/27 06:00 [entrez]
AID - ACAMC-EPUB-75959 [pii]
PST - ppublish
SO  - Anticancer Agents Med Chem. 2017;17(1):143-149.