PMID- 2722591
OWN - NLM
STAT- MEDLINE
DCOM- 19890713
LR  - 20190708
IS  - 0360-3016 (Print)
IS  - 0360-3016 (Linking)
VI  - 16
IP  - 6
DP  - 1989 Jun
TI  - Lack of a differential radiation response for proliferative and non-proliferative 
      rat thyroid cells (FRTL-5) in vitro.
PG  - 1511-7
AB  - FRTL-5 rat thyroid epithelial cells maintain normal thyroid function and 
      morphology in vitro, exhibit an absolute requirement for thyroid stimulating 
      hormone (TSH) for proliferation and display radiation dose response 
      characteristics indistinguishable from those of rat thyroid epithelial cells in 
      vivo (Rad. Res. 105:138-146, 1986). In TSH-free medium cells remain in a 
      non-proliferative, yet viable, state for prolonged periods of time and respond to 
      TSH re-stimulation by a return to exponential growth. Flow cytometric analysis 
      using two-step acridine orange (AO) staining revealed an accumulation of cells in 
      the G1 phase of the cell cycle accompanied by a pronounced reduction in red 
      fluorescence (indicative of RNA content) in FRTL-5 cells cultured in the absence 
      of TSH. The response of proliferative and non-proliferative FRTL-5 cells to 
      single dose, split dose and fractionated radiation was compared to determine 
      whether proliferative status was an important response determinant. The response 
      of FRTL-5 cells was not influenced by proliferative status at the time of 
      irradiation. Additionally, dose response was not altered by variable (12 hr-8 
      days) non-proliferative intervals before or after irradiation. As revealed by 
      split dose experiments, the rate and extent of sublethal damage repair was 
      likewise similar for proliferative and non-proliferative cells. Multifraction 
      experiments employing three fractions separated by 6 hr intervals indicate that 
      non-proliferative FRTL-5 cells completely repair sublethal damage between 
      fractions. These results indicate that the radiation response of FRTL-5 cells is 
      not influenced by the proliferative status of the cells prior to or 
      post-irradiation.
FAU - Brosing, J W
AU  - Brosing JW
AD  - Department of Human Oncology, University of Wisconsin, Madison 53792.
FAU - Giese, W L
AU  - Giese WL
FAU - Mulcahy, R T
AU  - Mulcahy RT
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Int J Radiat Oncol Biol Phys
JT  - International journal of radiation oncology, biology, physics
JID - 7603616
RN  - 9002-71-5 (Thyrotropin)
SB  - IM
MH  - Animals
MH  - Cell Cycle
MH  - Cell Division
MH  - Cell Line
MH  - Dose-Response Relationship, Radiation
MH  - In Vitro Techniques
MH  - Interphase
MH  - Rats
MH  - Thyroid Gland/cytology/*radiation effects
MH  - Thyrotropin/physiology
EDAT- 1989/06/01 00:00
MHDA- 1989/06/01 00:01
CRDT- 1989/06/01 00:00
PHST- 1989/06/01 00:00 [pubmed]
PHST- 1989/06/01 00:01 [medline]
PHST- 1989/06/01 00:00 [entrez]
AID - 0360-3016(89)90956-5 [pii]
AID - 10.1016/0360-3016(89)90956-5 [doi]
PST - ppublish
SO  - Int J Radiat Oncol Biol Phys. 1989 Jun;16(6):1511-7. doi: 
      10.1016/0360-3016(89)90956-5.