PMID- 27226085
OWN - NLM
STAT- MEDLINE
DCOM- 20170911
LR  - 20180426
IS  - 1862-8354 (Electronic)
IS  - 1862-8346 (Linking)
VI  - 10
IP  - 8
DP  - 2016 Aug
TI  - Protein translation occurs in platelet concentrates despite riboflavin/UV light 
      pathogen inactivation treatment.
PG  - 839-50
LID - 10.1002/prca.201500139 [doi]
AB  - PURPOSE: Pathogen inactivation technologies (PITs) were introduced into blood 
      banking to further improve the safety of blood products. However, the UV light 
      used in PITs to terminate pathogen growth might alter the functionality of the 
      cells in the blood product as well as the protein profile of the blood 
      components. This study employed proteomic approaches to assess changes in the 
      platelet proteome and translatome. EXPERIMENTAL DESIGN: Apheresis-derived 
      platelet concentrates treated with riboflavin/UV light or untreated controls were 
      analyzed throughout blood bank storage by quantitative proteomics using iTRAQ and 
      puromycin-associated nascent chain (PUNCH) proteomics. RESULTS: Quantitative 
      proteomic analysis identified 408 individual proteins including 26 unique 
      proteins that changed in the treated arm during storage. Proteomic results were 
      confirmed using immunoblot analyses and results suggested a translational control 
      of the protein expression profile. PUNCH proteomic analysis of day 7 samples from 
      illuminated units identified 52 unique platelet proteins that incorporated 
      puromycin, including proteins involved in the cytoskeleton, metabolism, and 
      signaling. CONCLUSION AND CLINICAL RELEVANCE: This study demonstrates for the 
      first time that platelets can synthesize proteins despite the riboflavin and UV 
      treatment and suggests that platelets may possess a mechanism to protect their 
      mRNA from damage by the PI treatment.
CI  - (c) 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Schubert, Peter
AU  - Schubert P
AD  - Centre for Innovation, Canadian Blood Services, University of British Columbia, 
      Vancouver, BC, Canada.
AD  - Centre for Blood Research, University of British Columbia, Vancouver, BC, Canada.
AD  - Department of Pathology and Laboratory Medicine, University of British Columbia, 
      Vancouver, BC, Canada.
FAU - Culibrk, Brankica
AU  - Culibrk B
AD  - Centre for Innovation, Canadian Blood Services, University of British Columbia, 
      Vancouver, BC, Canada.
AD  - Centre for Blood Research, University of British Columbia, Vancouver, BC, Canada.
FAU - Karwal, Simrath
AU  - Karwal S
AD  - Centre for Innovation, Canadian Blood Services, University of British Columbia, 
      Vancouver, BC, Canada.
AD  - Centre for Blood Research, University of British Columbia, Vancouver, BC, Canada.
FAU - Goodrich, Raymond P
AU  - Goodrich RP
AD  - TerumoBCT Biotechnologies, Lakewood, CO, USA.
FAU - Devine, Dana V
AU  - Devine DV
AD  - Centre for Innovation, Canadian Blood Services, University of British Columbia, 
      Vancouver, BC, Canada.
AD  - Centre for Blood Research, University of British Columbia, Vancouver, BC, Canada.
AD  - Department of Pathology and Laboratory Medicine, University of British Columbia, 
      Vancouver, BC, Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160621
PL  - Germany
TA  - Proteomics Clin Appl
JT  - Proteomics. Clinical applications
JID - 101298608
RN  - 0 (Blood Proteins)
RN  - TLM2976OFR (Riboflavin)
SB  - IM
MH  - Blood Platelets/drug effects/*metabolism/*microbiology/radiation effects
MH  - Blood Proteins/*biosynthesis
MH  - Humans
MH  - Microbial Viability/*drug effects/*radiation effects
MH  - Proteomics
MH  - Riboflavin/*pharmacology
MH  - *Ultraviolet Rays
OTO - NOTNLM
OT  - Blood platelets
OT  - Pathogen inactivation
OT  - Protein synthesis
OT  - Quantitative proteomics
OT  - Transfusion medicine
OT  - Translatome analyses
EDAT- 2016/05/27 06:00
MHDA- 2017/09/12 06:00
CRDT- 2016/05/27 06:00
PHST- 2015/12/01 00:00 [received]
PHST- 2016/05/15 00:00 [revised]
PHST- 2016/05/22 00:00 [accepted]
PHST- 2016/05/27 06:00 [entrez]
PHST- 2016/05/27 06:00 [pubmed]
PHST- 2017/09/12 06:00 [medline]
AID - 10.1002/prca.201500139 [doi]
PST - ppublish
SO  - Proteomics Clin Appl. 2016 Aug;10(8):839-50. doi: 10.1002/prca.201500139. Epub 
      2016 Jun 21.