PMID- 27227361
OWN - NLM
STAT- MEDLINE
DCOM- 20180730
LR  - 20181202
IS  - 1936-086X (Electronic)
IS  - 1936-0851 (Print)
IS  - 1936-0851 (Linking)
VI  - 10
IP  - 6
DP  - 2016 Jun 28
TI  - Scalable Production of Molybdenum Disulfide Based Biosensors.
PG  - 6173-9
LID - 10.1021/acsnano.6b02137 [doi]
AB  - We demonstrate arrays of opioid biosensors based on chemical vapor deposition 
      grown molybdenum disulfide (MoS2) field effect transistors (FETs) coupled to a 
      computationally redesigned, water-soluble variant of the mu-opioid receptor (MOR). 
      By transferring dense films of monolayer MoS2 crystals onto prefabricated 
      electrode arrays, we obtain high-quality FETs with clean surfaces that allow for 
      reproducible protein attachment. The fabrication yield of MoS2 FETs and 
      biosensors exceeds 95%, with an average mobility of 2.0 cm(2) V(-1) s(-1) (36 
      cm(2) V(-1) s(-1)) at room temperature under ambient (in vacuo). An atomic length 
      nickel-mediated linker chemistry enables target binding events that occur very 
      close to the MoS2 surface to maximize sensitivity. The biosensor response 
      calibration curve for a synthetic opioid peptide known to bind to the wild-type 
      MOR indicates binding affinity that matches values determined using traditional 
      techniques and a limit of detection  approximately 3 nM (1.5 ng/mL). The combination of 
      scalable array fabrication and rapid, precise binding readout enabled by the MoS2 
      transistor offers the prospect of a solid-state drug testing platform for rapid 
      readout of the interactions between novel drugs and their intended protein 
      targets.
FAU - Naylor, Carl H
AU  - Naylor CH
AD  - Department of Physics and Astronomy, double daggerDepartment of Anesthesiology and Critical 
      Care, and  section signDepartment of Chemistry, University of Pennsylvania , Philadelphia 
      19104, United States.
FAU - Kybert, Nicholas J
AU  - Kybert NJ
AD  - Department of Physics and Astronomy, double daggerDepartment of Anesthesiology and Critical 
      Care, and  section signDepartment of Chemistry, University of Pennsylvania , Philadelphia 
      19104, United States.
FAU - Schneier, Camilla
AU  - Schneier C
AD  - Department of Physics and Astronomy, double daggerDepartment of Anesthesiology and Critical 
      Care, and  section signDepartment of Chemistry, University of Pennsylvania , Philadelphia 
      19104, United States.
FAU - Xi, Jin
AU  - Xi J
AD  - Department of Physics and Astronomy, double daggerDepartment of Anesthesiology and Critical 
      Care, and  section signDepartment of Chemistry, University of Pennsylvania , Philadelphia 
      19104, United States.
FAU - Romero, Gabriela
AU  - Romero G
AD  - Department of Physics and Astronomy, double daggerDepartment of Anesthesiology and Critical 
      Care, and  section signDepartment of Chemistry, University of Pennsylvania , Philadelphia 
      19104, United States.
FAU - Saven, Jeffery G
AU  - Saven JG
AD  - Department of Physics and Astronomy, double daggerDepartment of Anesthesiology and Critical 
      Care, and  section signDepartment of Chemistry, University of Pennsylvania , Philadelphia 
      19104, United States.
FAU - Liu, Renyu
AU  - Liu R
AD  - Department of Physics and Astronomy, double daggerDepartment of Anesthesiology and Critical 
      Care, and  section signDepartment of Chemistry, University of Pennsylvania , Philadelphia 
      19104, United States.
FAU - Johnson, A T Charlie
AU  - Johnson AT
AD  - Department of Physics and Astronomy, double daggerDepartment of Anesthesiology and Critical 
      Care, and  section signDepartment of Chemistry, University of Pennsylvania , Philadelphia 
      19104, United States.
LA  - eng
GR  - K08 GM093115/GM/NIGMS NIH HHS/United States
GR  - R01 GM111421/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20160615
PL  - United States
TA  - ACS Nano
JT  - ACS nano
JID - 101313589
RN  - 0 (Analgesics, Opioid)
RN  - 0 (Disulfides)
RN  - 0 (Receptors, Opioid, mu)
RN  - 059QF0KO0R (Water)
RN  - 81AH48963U (Molybdenum)
RN  - ZC8B4P503V (molybdenum disulfide)
SB  - IM
MH  - Analgesics, Opioid/*analysis
MH  - *Biosensing Techniques
MH  - *Disulfides
MH  - *Molybdenum
MH  - Receptors, Opioid, mu
MH  - Water
PMC - PMC4949953
MID - NIHMS801382
OTO - NOTNLM
OT  - biosensors
OT  - monolayer MoS2
OT  - opioids
OT  - scalable
OT  - transition-metal dichalcogenide
COIS- Notes The authors declare no competing financial interest.
EDAT- 2016/05/27 06:00
MHDA- 2018/07/31 06:00
PMCR- 2016/07/19
CRDT- 2016/05/27 06:00
PHST- 2016/05/27 06:00 [entrez]
PHST- 2016/05/27 06:00 [pubmed]
PHST- 2018/07/31 06:00 [medline]
PHST- 2016/07/19 00:00 [pmc-release]
AID - 10.1021/acsnano.6b02137 [doi]
PST - ppublish
SO  - ACS Nano. 2016 Jun 28;10(6):6173-9. doi: 10.1021/acsnano.6b02137. Epub 2016 Jun 
      15.