PMID- 27227673
OWN - NLM
STAT- MEDLINE
DCOM- 20171023
LR  - 20181202
IS  - 1552-4965 (Electronic)
IS  - 1549-3296 (Linking)
VI  - 104
IP  - 10
DP  - 2016 Oct
TI  - Mechanoresponsiveness of human umbilical cord mesenchymal stem cells in in vitro 
      chondrogenesis-A comparative study with growth factor induction.
PG  - 2554-66
LID - 10.1002/jbm.a.35792 [doi]
AB  - Fetal-derived mesenchymal stem cells especially human umbilical cord matrix 
      mesenchymal stem cells (hUCMSCs), with their ease of availability, pluripotency, 
      and high expansion potential have emerged as an alternative solution for stem 
      cell based cartilage therapies. An attempt to elucidate the effect of dynamic 
      mechanical compression in modulating the chondrogenic differentiation of hUCMSCs 
      is done in this study to add on to the knowledge of optimizing chondrogenic 
      signals necessary for the effective differentiation of these stem cells and 
      subsequent integration to the surrounding tissues. hUCMSCs were seeded in porous 
      poly (vinyl alcohol)-poly (caprolactone) (PVA-PCL) scaffolds and cultured in 
      chondrogenic medium with/without TGF-beta3 and were subjected to a dynamic 
      compression of 10% strain, 1 Hz for 1/4 h for 7 days. The results on various 
      analysis shows that the extent of dynamic compression is an important factor 
      affecting cell viability. Mechanical stimulation in the form of dynamic 
      compression stimulates expression of chondrogenic genes even in the absence of 
      chondrogenic growth factors and also augments growth factor induced chondrogenic 
      potential of hUCMSC. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 
      104A: 2554-2566, 2016.
CI  - (c) 2016 Wiley Periodicals, Inc.
FAU - Remya, N S
AU  - Remya NS
AD  - Division of Tissue Engineering and Regeneration Technologies, BMT Wing, Sree 
      Chithra Tirunal Institute for Medical Sciences and Technology, Trivandrum, 
      695012, India.
FAU - Nair, Prabha D
AU  - Nair PD
AD  - Division of Tissue Engineering and Regeneration Technologies, BMT Wing, Sree 
      Chithra Tirunal Institute for Medical Sciences and Technology, Trivandrum, 
      695012, India.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20160614
PL  - United States
TA  - J Biomed Mater Res A
JT  - Journal of biomedical materials research. Part A
JID - 101234237
RN  - 0 (Polyesters)
RN  - 0 (Transforming Growth Factor beta3)
RN  - 24980-41-4 (polycaprolactone)
RN  - 9002-89-5 (Polyvinyl Alcohol)
SB  - IM
MH  - Cell Culture Techniques/methods
MH  - Cell Differentiation/drug effects
MH  - Cell Line
MH  - Chondrocytes/cytology/drug effects
MH  - Chondrogenesis/*drug effects
MH  - Equipment Design
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology/*drug effects/metabolism
MH  - Polyesters/chemistry
MH  - Polyvinyl Alcohol/analogs & derivatives
MH  - *Stress, Mechanical
MH  - Tissue Engineering/instrumentation/*methods
MH  - Tissue Scaffolds/*chemistry
MH  - Transforming Growth Factor beta3/*pharmacology
MH  - Umbilical Cord/cytology
OTO - NOTNLM
OT  - chondrogenic differentiation
OT  - dynamic compression
OT  - mechano transduction
OT  - mesenchymal stem cells
OT  - three dimensional scaffolds
EDAT- 2016/05/27 06:00
MHDA- 2017/10/24 06:00
CRDT- 2016/05/27 06:00
PHST- 2016/02/26 00:00 [received]
PHST- 2016/03/31 00:00 [revised]
PHST- 2016/05/24 00:00 [accepted]
PHST- 2016/05/27 06:00 [entrez]
PHST- 2016/05/27 06:00 [pubmed]
PHST- 2017/10/24 06:00 [medline]
AID - 10.1002/jbm.a.35792 [doi]
PST - ppublish
SO  - J Biomed Mater Res A. 2016 Oct;104(10):2554-66. doi: 10.1002/jbm.a.35792. Epub 
      2016 Jun 14.