PMID- 27230131
OWN - NLM
STAT- MEDLINE
DCOM- 20170619
LR  - 20191210
IS  - 1524-4636 (Electronic)
IS  - 1079-5642 (Print)
IS  - 1079-5642 (Linking)
VI  - 36
IP  - 7
DP  - 2016 Jul
TI  - Intracellular Localization of Endogenous Mouse ABCG1 Is Mimicked by Both 
      ABCG1-L550 and ABCG1-P550-Brief Report.
PG  - 1323-7
LID - 10.1161/ATVBAHA.116.307414 [doi]
AB  - OBJECTIVE: In a recent article in Arteriosclerosis, Thrombosis, and Vascular 
      Biology, it was reported that ATP-binding cassette transporter G1 (ABCG1) 
      containing leucine at position 550 (ABCG1-L550) was localized to the plasma 
      membrane, whereas ABCG1-P550 (proline at position 550) was intracellular. Because 
      the published data on the subcellular localization of ABCG1 are controversial, we 
      performed additional experiments to determine the importance of leucine or 
      proline at amino acid 550. APPROACH AND RESULTS: We transfected multiple cell 
      lines (CHO-K1, Cos-7, and HEK293 [human embryonic kidney]) with untagged or 
      FLAG-tagged ABCG1 containing either leucine or proline at position 550. 
      Immunofluorescence studies demonstrated that in all cases, ABCG1 localized to 
      intracellular endosomal vesicles. We also show that both ABCG1-L550 and 
      ABCG1-P550 are equally active in both promoting the efflux of cellular 
      cholesterol to exogenous high-density lipoprotein and in inducing the activity of 
      sterol regulatory element-binding protein-2, presumably as a result of 
      redistributing intracellular sterols away from the endoplasmic reticulum. 
      Importantly, we treated nontransfected primary peritoneal macrophages with a 
      liver X receptor agonist and demonstrate, using immunofluorescence, that although 
      endogenous ABCG1 localizes to intracellular endosomes, none was detectable at the 
      cell surface/plasma membrane. CONCLUSIONS: ABCG1, irrespective of either a 
      leucine or proline at position 550, is an intracellular protein that localizes to 
      vesicles of the endosomal pathway where it functions to mobilize sterols away 
      from the endoplasmic reticulum and out of the cell.
CI  - (c) 2016 American Heart Association, Inc.
FAU - Tarling, Elizabeth J
AU  - Tarling EJ
AD  - From the Departments of Biological Chemistry (P.A.E.) and Medicine (E.J.T.), 
      David Geffen School of Medicine at the University of California, Los Angeles. 
      etarling@mednet.ucla.edu.
FAU - Edwards, Peter A
AU  - Edwards PA
AD  - From the Departments of Biological Chemistry (P.A.E.) and Medicine (E.J.T.), 
      David Geffen School of Medicine at the University of California, Los Angeles.
LA  - eng
GR  - K99 HL118161/HL/NHLBI NIH HHS/United States
GR  - UL1 TR000124/TR/NCATS NIH HHS/United States
GR  - P30 DK063491/DK/NIDDK NIH HHS/United States
GR  - P01 HL030568/HL/NHLBI NIH HHS/United States
GR  - R00 HL118161/HL/NHLBI NIH HHS/United States
GR  - R01 DK102559/DK/NIDDK NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
DEP - 20160526
PL  - United States
TA  - Arterioscler Thromb Vasc Biol
JT  - Arteriosclerosis, thrombosis, and vascular biology
JID - 9505803
RN  - 0 (ABCG1 protein, mouse)
RN  - 0 (ATP Binding Cassette Transporter, Subfamily G, Member 1)
RN  - 0 (Cholesterol, HDL)
RN  - 0 (Liver X Receptors)
RN  - 0 (Srebf2 protein, mouse)
RN  - 0 (Sterol Regulatory Element Binding Protein 2)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - 9DLQ4CIU6V (Proline)
RN  - GMW67QNF9C (Leucine)
SB  - IM
MH  - ATP Binding Cassette Transporter, Subfamily G, Member 
      1/chemistry/deficiency/genetics/*metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Biological Transport
MH  - CHO Cells
MH  - COS Cells
MH  - Chlorocebus aethiops
MH  - Cholesterol/*metabolism
MH  - Cholesterol, HDL/metabolism
MH  - Cricetulus
MH  - Endosomes/*metabolism
MH  - Genotype
MH  - HEK293 Cells
MH  - Humans
MH  - Leucine
MH  - Liver X Receptors/agonists/metabolism
MH  - Macrophages, Peritoneal/drug effects/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Phenotype
MH  - Primary Cell Culture
MH  - Proline
MH  - Sterol Regulatory Element Binding Protein 2/metabolism
MH  - Transfection
PMC - PMC4919203
MID - NIHMS786844
OTO - NOTNLM
OT  - ABC transporter
OT  - cholesterol homeostasis
OT  - lipids
EDAT- 2016/05/28 06:00
MHDA- 2017/06/20 06:00
PMCR- 2017/07/01
CRDT- 2016/05/28 06:00
PHST- 2016/02/17 00:00 [received]
PHST- 2016/05/10 00:00 [accepted]
PHST- 2016/05/28 06:00 [entrez]
PHST- 2016/05/28 06:00 [pubmed]
PHST- 2017/06/20 06:00 [medline]
PHST- 2017/07/01 00:00 [pmc-release]
AID - ATVBAHA.116.307414 [pii]
AID - 10.1161/ATVBAHA.116.307414 [doi]
PST - ppublish
SO  - Arterioscler Thromb Vasc Biol. 2016 Jul;36(7):1323-7. doi: 
      10.1161/ATVBAHA.116.307414. Epub 2016 May 26.