PMID- 27230225
OWN - NLM
STAT- MEDLINE
DCOM- 20170509
LR  - 20191209
IS  - 1465-2099 (Electronic)
IS  - 0022-1317 (Linking)
VI  - 97
IP  - 8
DP  - 2016 Aug
TI  - Israeli acute paralysis virus associated paralysis symptoms, viral tissue 
      distribution and Dicer-2 induction in bumblebee workers (Bombus terrestris).
PG  - 1981-1989
LID - 10.1099/jgv.0.000516 [doi]
AB  - Although it is known that Israeli acute paralysis virus (IAPV) can cause bee 
      mortality, the symptoms of paralysis and the distribution of the virus in 
      different body tissues and their potential to respond with an increase of the 
      siRNA antiviral immune system have not been studied. In this project we worked 
      with Bombus terrestris, which is one of the most numerous bumblebee species in 
      Europe and an important pollinator for wild flowers and many crops in 
      agriculture. Besides the classic symptoms of paralysis and trembling prior to 
      death, we report a new IAPV-related symptom, crippled/immobilized forelegs. 
      Reverse-transcriptase quantitative PCR showed that IAPV accumulates in different 
      body tissues (midgut, fat body, brain and ovary). The highest levels of IAPV were 
      observed in the fat body. With fluorescence in situ hybridization (FISH) we 
      detected IAPV in the Kenyon cells of mushroom bodies and neuropils from both 
      antennal and optic lobes of the brain in IAPV-infected workers. Finally, we 
      observed an induction of Dicer-2, a core gene of the RNAi antiviral immune 
      response, in the IAPV-infected tissues of B. terrestris workers. According to our 
      results, tissue tropism and the induction strength of Dicer-2 could not be 
      correlated with virus-related paralysis symptoms.
FAU - Wang, Haidong
AU  - Wang H
AD  - Department of Crop Protection, Faculty of Bioscience Engineering, Ghent 
      University, Coupure Links 653, 9000 Ghent, Belgium.
FAU - Meeus, Ivan
AU  - Meeus I
AD  - Department of Crop Protection, Faculty of Bioscience Engineering, Ghent 
      University, Coupure Links 653, 9000 Ghent, Belgium.
FAU - Smagghe, Guy
AU  - Smagghe G
AD  - Department of Crop Protection, Faculty of Bioscience Engineering, Ghent 
      University, Coupure Links 653, 9000 Ghent, Belgium.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160526
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (RNA, Viral)
RN  - EC 3.1.26.3 (Ribonuclease III)
SB  - IM
MH  - Animal Structures/pathology/*virology
MH  - Animals
MH  - Bees/immunology/*virology
MH  - Dicistroviridae/*isolation & purification/*pathogenicity
MH  - Europe
MH  - In Situ Hybridization, Fluorescence
MH  - RNA Virus Infections/immunology/pathology/veterinary/virology
MH  - RNA, Viral/analysis
MH  - Real-Time Polymerase Chain Reaction
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Ribonuclease III/*analysis
EDAT- 2016/05/28 06:00
MHDA- 2017/05/10 06:00
CRDT- 2016/05/28 06:00
PHST- 2016/05/28 06:00 [entrez]
PHST- 2016/05/28 06:00 [pubmed]
PHST- 2017/05/10 06:00 [medline]
AID - 10.1099/jgv.0.000516 [doi]
PST - ppublish
SO  - J Gen Virol. 2016 Aug;97(8):1981-1989. doi: 10.1099/jgv.0.000516. Epub 2016 May 
      26.