PMID- 27231521
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160527
LR  - 20210109
IS  - 1949-8462 (Print)
IS  - 1949-8462 (Electronic)
VI  - 8
IP  - 5
DP  - 2016 May 26
TI  - Tenascin C upregulates interleukin-6 expression in human cardiac myofibroblasts 
      via toll-like receptor 4.
PG  - 340-50
LID - 10.4330/wjc.v8.i5.340 [doi]
AB  - AIM: To investigate the effect of Tenascin C (TNC) on the expression of 
      pro-inflammatory cytokines and matrix metalloproteinases in human cardiac 
      myofibroblasts (CMF). METHODS: CMF were isolated and cultured from patients 
      undergoing coronary artery bypass grafting. Cultured cells were treated with 
      either TNC (0.1 mumol/L, 24 h) or a recombinant protein corresponding to different 
      domains of the TNC protein; fibrinogen-like globe (FBG) and fibronectin type 
      III-like repeats (TNIII 5-7) (both 1 mumol/L, 24 h). The expression of the 
      pro-inflammatory cytokines; interleukin (IL)-6, IL-1beta, TNFalpha and the matrix 
      metalloproteinases; MMPs (MMP1, 2, 3, 9, 10, MT1-MMP) was assessed using real 
      time RT-PCR and western blot analysis. RESULTS: TNC increased both IL-6 and MMP3 
      (P < 0.01) mRNA levels in cultured human CMF but had no significant effect on the 
      other markers studied. The increase in IL-6 mRNA expression was mirrored by an 
      increase in protein secretion as assessed by enzyme-linked immunosorbant assay (P 
      < 0.01). Treating CMF with the recombinant protein FBG increased IL-6 mRNA and 
      protein (P < 0.01) whereas the recombinant protein TNIII 5-7 had no effect. 
      Neither FBG nor TNIII 5-7 had any significant effect on MMP3 expression. The 
      expression of toll-like receptor 4 (TLR4) in human CMF was confirmed by real time 
      RT-PCR, western blot and immunohistochemistry. Pre-incubation of cells with TLR4 
      neutralising antisera attenuated the effect of both TNC and FBG on IL-6 mRNA and 
      protein expression. CONCLUSION: TNC up-regulates IL-6 expression in human CMF, an 
      effect mediated through the FBG domain of TNC and via the TLR4 receptor.
FAU - Maqbool, Azhar
AU  - Maqbool A
AD  - Azhar Maqbool, Emma J Spary, Karen E Porter, Mark J Drinkhill, Neil A Turner, 
      Division of Cardiovascular and Diabetes Research, Leeds Institute of 
      Cardiovascular and Metabolic Medicine, School of Medicine, University of Leeds, 
      Leeds LS2 9JT, United Kingdom.
FAU - Spary, Emma J
AU  - Spary EJ
AD  - Azhar Maqbool, Emma J Spary, Karen E Porter, Mark J Drinkhill, Neil A Turner, 
      Division of Cardiovascular and Diabetes Research, Leeds Institute of 
      Cardiovascular and Metabolic Medicine, School of Medicine, University of Leeds, 
      Leeds LS2 9JT, United Kingdom.
FAU - Manfield, Iain W
AU  - Manfield IW
AD  - Azhar Maqbool, Emma J Spary, Karen E Porter, Mark J Drinkhill, Neil A Turner, 
      Division of Cardiovascular and Diabetes Research, Leeds Institute of 
      Cardiovascular and Metabolic Medicine, School of Medicine, University of Leeds, 
      Leeds LS2 9JT, United Kingdom.
FAU - Ruhmann, Michaela
AU  - Ruhmann M
AD  - Azhar Maqbool, Emma J Spary, Karen E Porter, Mark J Drinkhill, Neil A Turner, 
      Division of Cardiovascular and Diabetes Research, Leeds Institute of 
      Cardiovascular and Metabolic Medicine, School of Medicine, University of Leeds, 
      Leeds LS2 9JT, United Kingdom.
FAU - Zuliani-Alvarez, Lorena
AU  - Zuliani-Alvarez L
AD  - Azhar Maqbool, Emma J Spary, Karen E Porter, Mark J Drinkhill, Neil A Turner, 
      Division of Cardiovascular and Diabetes Research, Leeds Institute of 
      Cardiovascular and Metabolic Medicine, School of Medicine, University of Leeds, 
      Leeds LS2 9JT, United Kingdom.
FAU - Gamboa-Esteves, Filomena O
AU  - Gamboa-Esteves FO
AD  - Azhar Maqbool, Emma J Spary, Karen E Porter, Mark J Drinkhill, Neil A Turner, 
      Division of Cardiovascular and Diabetes Research, Leeds Institute of 
      Cardiovascular and Metabolic Medicine, School of Medicine, University of Leeds, 
      Leeds LS2 9JT, United Kingdom.
FAU - Porter, Karen E
AU  - Porter KE
AD  - Azhar Maqbool, Emma J Spary, Karen E Porter, Mark J Drinkhill, Neil A Turner, 
      Division of Cardiovascular and Diabetes Research, Leeds Institute of 
      Cardiovascular and Metabolic Medicine, School of Medicine, University of Leeds, 
      Leeds LS2 9JT, United Kingdom.
FAU - Drinkhill, Mark J
AU  - Drinkhill MJ
AD  - Azhar Maqbool, Emma J Spary, Karen E Porter, Mark J Drinkhill, Neil A Turner, 
      Division of Cardiovascular and Diabetes Research, Leeds Institute of 
      Cardiovascular and Metabolic Medicine, School of Medicine, University of Leeds, 
      Leeds LS2 9JT, United Kingdom.
FAU - Midwood, Kim S
AU  - Midwood KS
AD  - Azhar Maqbool, Emma J Spary, Karen E Porter, Mark J Drinkhill, Neil A Turner, 
      Division of Cardiovascular and Diabetes Research, Leeds Institute of 
      Cardiovascular and Metabolic Medicine, School of Medicine, University of Leeds, 
      Leeds LS2 9JT, United Kingdom.
FAU - Turner, Neil A
AU  - Turner NA
AD  - Azhar Maqbool, Emma J Spary, Karen E Porter, Mark J Drinkhill, Neil A Turner, 
      Division of Cardiovascular and Diabetes Research, Leeds Institute of 
      Cardiovascular and Metabolic Medicine, School of Medicine, University of Leeds, 
      Leeds LS2 9JT, United Kingdom.
LA  - eng
GR  - 20003/VAC_/Versus Arthritis/United Kingdom
PT  - Journal Article
PL  - United States
TA  - World J Cardiol
JT  - World journal of cardiology
JID - 101537090
PMC - PMC4877363
OTO - NOTNLM
OT  - Cardiac fibroblasts
OT  - Interleukin-6
OT  - Matrix metalloproteinase
OT  - Tenascin C
OT  - Toll-like receptor
EDAT- 2016/05/28 06:00
MHDA- 2016/05/28 06:01
PMCR- 2016/05/26
CRDT- 2016/05/28 06:00
PHST- 2016/03/03 00:00 [received]
PHST- 2016/04/04 00:00 [revised]
PHST- 2016/04/14 00:00 [accepted]
PHST- 2016/05/28 06:00 [entrez]
PHST- 2016/05/28 06:00 [pubmed]
PHST- 2016/05/28 06:01 [medline]
PHST- 2016/05/26 00:00 [pmc-release]
AID - 10.4330/wjc.v8.i5.340 [doi]
PST - ppublish
SO  - World J Cardiol. 2016 May 26;8(5):340-50. doi: 10.4330/wjc.v8.i5.340.