PMID- 27231847
OWN - NLM
STAT- MEDLINE
DCOM- 20180227
LR  - 20181202
IS  - 1949-2553 (Electronic)
IS  - 1949-2553 (Linking)
VI  - 7
IP  - 25
DP  - 2016 Jun 21
TI  - EAAT3 promotes amino acid transport and proliferation of porcine intestinal 
      epithelial cells.
PG  - 38681-38692
LID - 10.18632/oncotarget.9583 [doi]
AB  - Excitatory amino acid transporter 3 (EAAT3, encoded by SLC1A1) is an epithelial 
      type high-affinity anionic amino acid transporter, and glutamate is the major 
      oxidative fuel for intestinal epithelial cells. This study investigated the 
      effects of EAAT3 on amino acid transport and cell proliferation through 
      activation of the mammalian target of the rapamycin (mTOR) pathway in porcine 
      jejunal epithelial cells (IPEC-J2). Anionic amino acid and cystine (Cys) 
      transport were increased (P<0.05) by EAAT3 overexpression and decreased (P<0.05) 
      by EAAT3 knockdown rather than other amino acids. MTT and cell counting assays 
      suggested that IPEC-J2 cell proliferation increased (P<0.05) with EAAT3 
      overexpression. Phosphorylation of mTOR (Ser2448), ribosomal protein S6 kinase-1 
      (S6K1, Thr389) and eukaryotic initiation factor 4E-binding protein-1 (4EBP1, 
      Thr70) was increased by EAAT3 overexpression and decreased by EAAT3 knockdown 
      (P<0.05), as were levels of activating transcription factor 4 (ATF4) and 
      cystine/glutamate antiporter (xCT) (P<0.05). Our results demonstrate for the 
      first time that EAAT3 facilitates anionic amino acid transport and activates the 
      mTOR pathway, promoting Cys transport and IPEC-J2 cell proliferation.
FAU - Ye, Jin-Ling
AU  - Ye JL
AD  - College of Animal Science, South China Agricultural University/National 
      Engineering Research Center for Breeding Swine Industry, Guangzhou, Guangdong 
      Province, China.
FAU - Gao, Chun-Qi
AU  - Gao CQ
AD  - College of Animal Science, South China Agricultural University/National 
      Engineering Research Center for Breeding Swine Industry, Guangzhou, Guangdong 
      Province, China.
FAU - Li, Xiang-Guang
AU  - Li XG
AD  - College of Animal Science, South China Agricultural University/National 
      Engineering Research Center for Breeding Swine Industry, Guangzhou, Guangdong 
      Province, China.
FAU - Jin, Cheng-Long
AU  - Jin CL
AD  - College of Animal Science, South China Agricultural University/National 
      Engineering Research Center for Breeding Swine Industry, Guangzhou, Guangdong 
      Province, China.
FAU - Wang, Dan
AU  - Wang D
AD  - College of Animal Science, South China Agricultural University/National 
      Engineering Research Center for Breeding Swine Industry, Guangzhou, Guangdong 
      Province, China.
FAU - Shu, Gang
AU  - Shu G
AD  - College of Animal Science, South China Agricultural University/National 
      Engineering Research Center for Breeding Swine Industry, Guangzhou, Guangdong 
      Province, China.
FAU - Wang, Wen-Ce
AU  - Wang WC
AD  - College of Animal Science, South China Agricultural University/National 
      Engineering Research Center for Breeding Swine Industry, Guangzhou, Guangdong 
      Province, China.
FAU - Kong, Xiang-Feng
AU  - Kong XF
AD  - Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 
      Hunan Province, China.
FAU - Yao, Kang
AU  - Yao K
AD  - Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 
      Hunan Province, China.
FAU - Yan, Hui-Chao
AU  - Yan HC
AD  - College of Animal Science, South China Agricultural University/National 
      Engineering Research Center for Breeding Swine Industry, Guangzhou, Guangdong 
      Province, China.
FAU - Wang, Xiu-Qi
AU  - Wang XQ
AD  - College of Animal Science, South China Agricultural University/National 
      Engineering Research Center for Breeding Swine Industry, Guangzhou, Guangdong 
      Province, China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Oncotarget
JT  - Oncotarget
JID - 101532965
RN  - 0 (Excitatory Amino Acid Transporter 3)
SB  - IM
MH  - Animals
MH  - Cell Proliferation/physiology
MH  - Cells, Cultured
MH  - Epithelial Cells/*metabolism
MH  - Excitatory Amino Acid Transporter 3/biosynthesis/*metabolism
MH  - Female
MH  - Humans
MH  - Intestinal Mucosa/*metabolism
MH  - Intestines/cytology
MH  - Male
MH  - Swine
PMC - PMC5122420
OTO - NOTNLM
OT  - amino acid
OT  - excitatory amino acid transporter 3
OT  - intestinal epithelial cells
OT  - mammalian target of rapamycin
OT  - proliferation
COIS- The authors declare no conflicts of interest.
EDAT- 2016/10/23 06:00
MHDA- 2018/02/28 06:00
PMCR- 2016/06/21
CRDT- 2016/05/28 06:00
PHST- 2016/02/12 00:00 [received]
PHST- 2016/04/29 00:00 [accepted]
PHST- 2016/10/23 06:00 [pubmed]
PHST- 2018/02/28 06:00 [medline]
PHST- 2016/05/28 06:00 [entrez]
PHST- 2016/06/21 00:00 [pmc-release]
AID - 9583 [pii]
AID - 10.18632/oncotarget.9583 [doi]
PST - ppublish
SO  - Oncotarget. 2016 Jun 21;7(25):38681-38692. doi: 10.18632/oncotarget.9583.