PMID- 27234424
OWN - NLM
STAT- MEDLINE
DCOM- 20170926
LR  - 20180321
IS  - 1096-0805 (Electronic)
IS  - 0022-2011 (Linking)
VI  - 137
DP  - 2016 Jun
TI  - Ostreid herpesvirus type 1 genomic diversity in wild populations of Pacific 
      oyster Crassostrea gigas from Italian coasts.
PG  - 71-83
LID - S0022-2011(16)30051-9 [pii]
LID - 10.1016/j.jip.2016.05.004 [doi]
AB  - Ostreid herpesvirus 1 (OsHV-1) is a significant pathogen affecting the young 
      Pacific oyster Crassostrea gigas, worldwide. A new variant, OsHV-1 muVar, has been 
      associated with recurrent mortality events in Europe since 2008. Epidemiological 
      data collection is key for global risk assessment; however little is known about 
      health status and genotypes present in European wild oyster beds. Most studies to 
      date have involved only cultivated individuals during mortality events, and 
      reported low genotype diversity. With this study, conducted along the Italian 
      coasts, we investigated for the first time the presence of OsHV-1 in European 
      natural oyster beds. Analysis of three genomic regions revealed the presence of 
      at least nine different genotypes, including two variants close to the OsHV-1 
      reference, known since the early 1990s but with no European record reported since 
      2010, and highlights relevant genotype diversity in natural environment. 
      Phylogenetic analysis distinguished two distinct clusters and geographical 
      distribution of genotypes, with the exception of a variant very closely related 
      to the muVar, which appeared the single genotype present in all the Adriatic 
      sites. Interestingly, these wild symptom free populations could represent, in 
      Europe, an accessible alternative to the import of OsHV-1-resistant oyster 
      strains from the East Pacific, the native area of C. gigas, avoiding the 
      high-risk of non-native marine species and new pathogen introductions.
CI  - Copyright (c) 2016 Elsevier Inc. All rights reserved.
FAU - Burioli, E A V
AU  - Burioli EAV
AD  - Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, 
      Torino, Italy; Department of Veterinary Medical Sciences, Alma Mater Studiorum, 
      Universita di Bologna, Ozzano dell'Emilia, BO, Italy. Electronic address: 
      erika.burioli@izsto.it.
FAU - Prearo, M
AU  - Prearo M
AD  - Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, 
      Torino, Italy.
FAU - Riina, M V
AU  - Riina MV
AD  - Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, 
      Torino, Italy.
FAU - Bona, M C
AU  - Bona MC
AD  - Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, 
      Torino, Italy.
FAU - Fioravanti, M L
AU  - Fioravanti ML
AD  - Department of Veterinary Medical Sciences, Alma Mater Studiorum, Universita di 
      Bologna, Ozzano dell'Emilia, BO, Italy.
FAU - Arcangeli, G
AU  - Arcangeli G
AD  - Istituto Zooprofilattico Sperimentale delle Venezie, Legnaro, PD, Italy.
FAU - Houssin, M
AU  - Houssin M
AD  - Laboratoire Frank Duncombe LABEO, Caen, France.
LA  - eng
PT  - Journal Article
DEP - 20160524
PL  - United States
TA  - J Invertebr Pathol
JT  - Journal of invertebrate pathology
JID - 0014067
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - Crassostrea/*virology
MH  - DNA, Viral/analysis
MH  - Genes, Viral
MH  - Genotype
MH  - Herpesviridae/*genetics
MH  - Herpesviridae Infections/*veterinary
MH  - Italy
MH  - Phylogeny
MH  - Polymerase Chain Reaction
MH  - Prevalence
MH  - Sequence Analysis, DNA
OTO - NOTNLM
OT  - Bivalves
OT  - Crassostrea gigas
OT  - Ostreid herpesvirus-1
OT  - Variants
OT  - Wild population
EDAT- 2016/05/29 06:00
MHDA- 2017/09/28 06:00
CRDT- 2016/05/29 06:00
PHST- 2016/02/23 00:00 [received]
PHST- 2016/05/19 00:00 [revised]
PHST- 2016/05/21 00:00 [accepted]
PHST- 2016/05/29 06:00 [entrez]
PHST- 2016/05/29 06:00 [pubmed]
PHST- 2017/09/28 06:00 [medline]
AID - S0022-2011(16)30051-9 [pii]
AID - 10.1016/j.jip.2016.05.004 [doi]
PST - ppublish
SO  - J Invertebr Pathol. 2016 Jun;137:71-83. doi: 10.1016/j.jip.2016.05.004. Epub 2016 
      May 24.