PMID- 27234553
OWN - NLM
STAT- MEDLINE
DCOM- 20170203
LR  - 20210331
IS  - 1532-2661 (Electronic)
IS  - 0034-5288 (Linking)
VI  - 106
DP  - 2016 Jun
TI  - Effects of inactivated porcine epidemic diarrhea virus on porcine 
      monocyte-derived dendritic cells and intestinal dendritic cells.
PG  - 149-58
LID - S0034-5288(16)30067-4 [pii]
LID - 10.1016/j.rvsc.2016.03.023 [doi]
AB  - Porcine epidemic diarrhea (PED) is a serious infection in neonatal piglets. As 
      the causative agent of PED, porcine epidemic diarrhea virus (PEDV) results in 
      acute diarrhea and dehydration with high mortality rates in swine. Dendritic 
      cells (DCs) are highly effective antigen-presenting cells to uptake and present 
      viral antigens to T cells, which then initiate a distinct immune response. In 
      this study, our results show that the expression of Mo-DCs surface markers such 
      as SWC3a(+)CD1a(+), SWC3a(+)CD80/86(+) and SWC3a(+)SLA-II-DR(+) is increased 
      after incubation with UV-PEDV for 24h. Mo-DCs incubated with UV-PEDV produce 
      higher levels of IL-12 and INF-gamma compared to mock-infected Mo-DCs. Interactions 
      between Mo-DCs and UV-PEDV significantly stimulate T-cell proliferation in vitro. 
      Consistent with these results, there is an enhancement in the ability of porcine 
      intestinal DCs to activate T-cell proliferation in vivo. We conclude that UV-PEDV 
      may be a useful and safe vaccine to trigger adaptive immunity.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Gao, Qi
AU  - Gao Q
AD  - Nanjing Agricultural University, Wei gang 1, Jiangsu, PR China.
FAU - Zhao, Shanshan
AU  - Zhao S
AD  - Nanjing Agricultural University, Wei gang 1, Jiangsu, PR China.
FAU - Qin, Tao
AU  - Qin T
AD  - Nanjing Agricultural University, Wei gang 1, Jiangsu, PR China.
FAU - Yin, Yinyan
AU  - Yin Y
AD  - Nanjing Agricultural University, Wei gang 1, Jiangsu, PR China.
FAU - Yu, Qinghua
AU  - Yu Q
AD  - Nanjing Agricultural University, Wei gang 1, Jiangsu, PR China.
FAU - Yang, Qian
AU  - Yang Q
AD  - Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing 
      Agricultural University, PR China. Electronic address: zxbyq@njau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20160414
PL  - England
TA  - Res Vet Sci
JT  - Research in veterinary science
JID - 0401300
RN  - 0 (Vaccines, Inactivated)
RN  - 0 (Viral Vaccines)
SB  - IM
EIN - Res Vet Sci. 2020 Oct;132:28-32. PMID: 32474262
MH  - Animals
MH  - Coronavirus Infections/immunology/*veterinary/virology
MH  - Dendritic Cells/*immunology
MH  - Diarrhea/immunology/*veterinary/virology
MH  - Intestines/immunology
MH  - Lymphocyte Activation/immunology
MH  - Monocytes/immunology
MH  - Porcine epidemic diarrhea virus/*immunology
MH  - Swine
MH  - Swine Diseases/*immunology/virology
MH  - T-Lymphocytes/immunology
MH  - Vaccines, Inactivated/immunology
MH  - Viral Vaccines/*immunology
OTO - NOTNLM
OT  - Immunity
OT  - Inactivated porcine epidemic diarrhea virus
OT  - Intestinal DCs
OT  - Monocyte-derived dendritic cells (Mo-DCs)
OT  - T cell proliferation
EDAT- 2016/05/29 06:00
MHDA- 2017/02/06 06:00
CRDT- 2016/05/29 06:00
PHST- 2015/06/16 00:00 [received]
PHST- 2016/03/16 00:00 [revised]
PHST- 2016/03/28 00:00 [accepted]
PHST- 2016/05/29 06:00 [entrez]
PHST- 2016/05/29 06:00 [pubmed]
PHST- 2017/02/06 06:00 [medline]
AID - S0034-5288(16)30067-4 [pii]
AID - 10.1016/j.rvsc.2016.03.023 [doi]
PST - ppublish
SO  - Res Vet Sci. 2016 Jun;106:149-58. doi: 10.1016/j.rvsc.2016.03.023. Epub 2016 Apr 
      14.