PMID- 27234556
OWN - NLM
STAT- MEDLINE
DCOM- 20170203
LR  - 20210119
IS  - 1532-2661 (Electronic)
IS  - 0034-5288 (Print)
IS  - 0034-5288 (Linking)
VI  - 106
DP  - 2016 Jun
TI  - Cloning and characterization of giant panda (Ailuropoda melanoleuca) IL-18 
      binding protein.
PG  - 170-2
LID - S0034-5288(16)30072-8 [pii]
LID - 10.1016/j.rvsc.2016.04.004 [doi]
AB  - The giant panda (Ailuropoda melanoleuca) is an endangered species. Interleukin-18 
      (IL-18) plays an important role in the innate and adaptive immune responses by 
      inducing IFN-gamma. IL-18 has been implicated in the pathogenesis of various 
      diseases. IL-18 binding protein (IL-18BP) is an intrinsic inhibitor of IL-18 that 
      possesses higher affinity to IL-18. In this study, we cloned and characterized 
      IL-18BP in giant panda (AmIL-18BP) from the spleen. The amino acid sequence of 
      giant panda IL-18BP ORF shared about 65% identities with other species. To 
      evaluate the effects of AmIL-18BP on the immune responses, we expressed the 
      recombinant AmIL-18BP in Escherichia coli BL21 (DE3).The fusing protein 
      PET-AmIL-18BP was purified by nickel affinity column chromatography. The 
      biological function of purified PET-AmIL-18BP was determined on mice splenocyte 
      by qRT-PCR. The results showed that AmIL-18BP was functional and could 
      significantly reduce IFN-gamma production in murine splenocytes. These results will 
      facilitate the study of protecting giant panda on etiology and immunology.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Yan, Yue
AU  - Yan Y
AD  - College of Life Sciences, Sichuan University, Key Laboratory for Bio-Resources 
      and Eco-Environment of Ministry of Education, Sichuan Key Laboratory of Molecular 
      Biology and Biotechnology, Chengdu 610064, PR China.
FAU - Deng, Jiabo
AU  - Deng J
AD  - Chengdu Zoo, Chengdu 610081, PR China.
FAU - Niu, Lili
AU  - Niu L
AD  - Chengdu Zoo, Chengdu 610081, PR China.
FAU - Wang, Qiang
AU  - Wang Q
AD  - Chengdu Zoo, Chengdu 610081, PR China.
FAU - Yu, Jianqiu
AU  - Yu J
AD  - Chengdu Zoo, Chengdu 610081, PR China.
FAU - Shao, Huanhuan
AU  - Shao H
AD  - College of Life Sciences, Sichuan University, Key Laboratory for Bio-Resources 
      and Eco-Environment of Ministry of Education, Sichuan Key Laboratory of Molecular 
      Biology and Biotechnology, Chengdu 610064, PR China.
FAU - Cao, Qinghua
AU  - Cao Q
AD  - College of Life Sciences, Sichuan University, Key Laboratory for Bio-Resources 
      and Eco-Environment of Ministry of Education, Sichuan Key Laboratory of Molecular 
      Biology and Biotechnology, Chengdu 610064, PR China.
FAU - Zhang, Yizheng
AU  - Zhang Y
AD  - College of Life Sciences, Sichuan University, Key Laboratory for Bio-Resources 
      and Eco-Environment of Ministry of Education, Sichuan Key Laboratory of Molecular 
      Biology and Biotechnology, Chengdu 610064, PR China.
FAU - Tan, Xuemei
AU  - Tan X
AD  - College of Life Sciences, Sichuan University, Key Laboratory for Bio-Resources 
      and Eco-Environment of Ministry of Education, Sichuan Key Laboratory of Molecular 
      Biology and Biotechnology, Chengdu 610064, PR China. Electronic address: 
      txmyyf@scu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20160427
PL  - England
TA  - Res Vet Sci
JT  - Research in veterinary science
JID - 0401300
RN  - 0 (DNA, Complementary)
RN  - 0 (IFNG protein, mouse)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (interleukin-18 binding protein)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics/metabolism
MH  - Intercellular Signaling Peptides and Proteins/chemistry/*genetics/metabolism
MH  - Interferon-gamma/genetics/metabolism
MH  - Mice
MH  - Sequence Analysis, Protein/veterinary
MH  - Spleen/immunology
MH  - Ursidae/*genetics/immunology
PMC - PMC7111782
OTO - NOTNLM
OT  - Bioactivity analysis
OT  - Giant panda
OT  - IL-18 binding protein
OT  - Interleukin-18
EDAT- 2016/05/29 06:00
MHDA- 2017/02/06 06:00
PMCR- 2016/04/27
CRDT- 2016/05/29 06:00
PHST- 2015/04/20 00:00 [received]
PHST- 2016/03/31 00:00 [revised]
PHST- 2016/04/25 00:00 [accepted]
PHST- 2016/05/29 06:00 [entrez]
PHST- 2016/05/29 06:00 [pubmed]
PHST- 2017/02/06 06:00 [medline]
PHST- 2016/04/27 00:00 [pmc-release]
AID - S0034-5288(16)30072-8 [pii]
AID - 10.1016/j.rvsc.2016.04.004 [doi]
PST - ppublish
SO  - Res Vet Sci. 2016 Jun;106:170-2. doi: 10.1016/j.rvsc.2016.04.004. Epub 2016 Apr 
      27.