PMID- 27235551
OWN - NLM
STAT- MEDLINE
DCOM- 20170523
LR  - 20211204
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 476
IP  - 4
DP  - 2016 Aug 5
TI  - mTOR remains unchanged in diet-resistant (DR) rats despite impaired LKB1/AMPK 
      cascade in adipose tissue.
PG  - 333-339
LID - S0006-291X(16)30838-5 [pii]
LID - 10.1016/j.bbrc.2016.05.123 [doi]
AB  - Liver kinase B1 (LKB1) plays an important role in adipogenesis, but the 
      underlying molecular mechanism is poorly understood. Here, we explored the 
      functional relationship between LKB1 and the mammalian target of rapamycin (mTOR) 
      in regulating adipogenesis in rats and preadipocytes. We found that LKB1 and the 
      adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK) cascade are 
      impaired in the white adipose tissue (WAT) of diet-induced obesity (DIO) and 
      diet-resistant (DR) rats when compared with chow-fed (CF) rats. While DIO 
      activated the mTOR pathway in WAT and led to a more fat mass gain, DR maintained 
      the normal activity of the mTOR pathway and normal weight and percentage of fat 
      mass. We further constructed overexpressed LKB1 (OE) and silenced LKB1 (Si) 
      3T3-L1 preadipocytes monoclonal cell lines. In the OE cell line, the mTOR pathway 
      was inactivated, and intracellular lipid content was reduced during 
      differentiation. This effect could be reversed by AMPK inhibition. Conversely, in 
      the Si cell line, the mTOR pathway was activated and intracellular lipid content 
      increased. This effect could be reversed by rapamycin, an inhibitor of mTOR. Our 
      results suggest that mTOR mediates the effect of LKB1 on adipogenesis, and normal 
      activity of mTOR in DR rats interferes with the effect of LKB1 in WAT.
CI  - Copyright (c) 2016 Elsevier Inc. All rights reserved.
FAU - Han, Jie
AU  - Han J
AD  - Department of Human Anatomy and Histology, Tianjin Medical University, Tianjin 
      300070, China.
FAU - Liang, Huimin
AU  - Liang H
AD  - Department of Human Anatomy and Histology, Tianjin Medical University, Tianjin 
      300070, China.
FAU - Tian, Derun
AU  - Tian D
AD  - Department of Human Anatomy and Histology, Tianjin Medical University, Tianjin 
      300070, China. Electronic address: tiandr@tmu.edu.cn.
FAU - Du, Jianying
AU  - Du J
AD  - Department of Human Anatomy and Histology, Tianjin Medical University, Tianjin 
      300070, China.
FAU - Wang, Qiming
AU  - Wang Q
AD  - Department of Human Anatomy and Histology, Tianjin Medical University, Tianjin 
      300070, China.
FAU - Xi, Pengjiao
AU  - Xi P
AD  - Department of Human Anatomy and Histology, Tianjin Medical University, Tianjin 
      300070, China.
FAU - Wang, Haomin
AU  - Wang H
AD  - Department of Human Anatomy and Histology, Tianjin Medical University, Tianjin 
      300070, China.
FAU - Li, Yongmei
AU  - Li Y
AD  - Department of Human Anatomy and Histology, Tianjin Medical University, Tianjin 
      300070, China.
LA  - eng
PT  - Journal Article
DEP - 20160525
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (lysis protein, E coli)
RN  - EC 2.7.1.1 (mTOR protein, rat)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (Stk11 protein, rat)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 2.7.11.3 (AMP-Activated Protein Kinase Kinases)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
SB  - IM
MH  - 3T3-L1 Cells
MH  - AMP-Activated Protein Kinase Kinases
MH  - AMP-Activated Protein Kinases/*metabolism
MH  - Adipocytes/physiology
MH  - Adipogenesis/physiology
MH  - Adipose Tissue/*metabolism
MH  - Animals
MH  - Body Composition
MH  - Body Weight
MH  - Cell Line
MH  - Diet, High-Fat/adverse effects
MH  - Escherichia coli Proteins
MH  - Male
MH  - Mice
MH  - Obesity/etiology/metabolism
MH  - Protein Serine-Threonine Kinases/genetics/*metabolism
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction
MH  - TOR Serine-Threonine Kinases/*metabolism
OTO - NOTNLM
OT  - Adipogenesis
OT  - Diet-induced obesity
OT  - Diet-resistant
OT  - LKB1
OT  - Preadipocytes
OT  - mTOR
EDAT- 2016/05/29 06:00
MHDA- 2017/05/24 06:00
CRDT- 2016/05/29 06:00
PHST- 2016/05/21 00:00 [received]
PHST- 2016/05/24 00:00 [accepted]
PHST- 2016/05/29 06:00 [entrez]
PHST- 2016/05/29 06:00 [pubmed]
PHST- 2017/05/24 06:00 [medline]
AID - S0006-291X(16)30838-5 [pii]
AID - 10.1016/j.bbrc.2016.05.123 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2016 Aug 5;476(4):333-339. doi: 
      10.1016/j.bbrc.2016.05.123. Epub 2016 May 25.