PMID- 27235775
OWN - NLM
STAT- MEDLINE
DCOM- 20170505
LR  - 20171128
IS  - 1879-2448 (Electronic)
IS  - 0043-1354 (Linking)
VI  - 100
DP  - 2016 Sep 1
TI  - Chronic impact of sulfamethoxazole on the metabolic activity and composition of 
      enriched nitrifying microbial culture.
PG  - 546-555
LID - S0043-1354(16)30370-0 [pii]
LID - 10.1016/j.watres.2016.05.043 [doi]
AB  - This study investigated the chronic impact of sulfamethoxazole (SMX) on activated 
      sludge sustaining an enriched nitrifying biomass. For this purpose, a laboratory 
      scale fill and draw reactor was operated with 100 mg COD/L of peptone mixture and 
      50 mg N/L of ammonia at a sludge age of 15 days. Additionally, the biomass was 
      exposed to a daily SMX dose of 50 mg/L once the reactor reached steady-state 
      conditions. The reactor performance and microbial composition were monitored for 
      37 days with conventional parameters and molecular techniques based on the gene 
      for ammonia monooxygenase subunit A (amoA) and the prokaryotic 16S rRNA gene. 
      Denaturing gradient gel electrophoresis (DGGE) and 16S rRNA gene cloning analyses 
      suggested a microbial community change concurrent with the addition of SMX. 
      Specifically, quantitative polymerase chain reaction analyses (qPCR/RT-qPCR) 
      revealed a significant reduction in the levels and activity of ammonia oxidizing 
      bacteria (AOB). However, the acclimation period ended with high amoA mRNA levels 
      and improved nitrification efficiency. Partial degradation of SMX by 
      heterotrophic bacteria was also observed.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Katipoglu-Yazan, Tugce
AU  - Katipoglu-Yazan T
AD  - Istanbul Technical University, Faculty of Civil Engineering, Environmental 
      Engineering Department, 34469 Maslak, Istanbul, Turkey; CNRS, Laboratoire de 
      Chimie Physique et Microbiologie pour l'Environnement (LCPME), UMR 7564, Institut 
      Jean Barriol, 15 Avenue du Charmois, 54500 Vandoeuvre-les-Nancy, France. 
      Electronic address: tugce.katipoglu@gmail.com.
FAU - Merlin, Christophe
AU  - Merlin C
AD  - CNRS, Laboratoire de Chimie Physique et Microbiologie pour l'Environnement 
      (LCPME), UMR 7564, Institut Jean Barriol, 15 Avenue du Charmois, 54500 
      Vandoeuvre-les-Nancy, France; Universite de Lorraine, LCPME, UMR 7564, 15 Avenue 
      du Charmois, 54500 Vandoeuvre-les-Nancy, France. Electronic address: 
      christophe.merlin@univ-lorraine.fr.
FAU - Pons, Marie-Noelle
AU  - Pons MN
AD  - Laboratoire Reactions et Genie des Procedes (UMR 7274 CNRS) Universite de 
      Lorraine, 1 rue Grandville, BP 20451, 54001 Nancy Cedex, France. Electronic 
      address: marie-noelle.pons@univ-lorraine.fr.
FAU - Ubay-Cokgor, Emine
AU  - Ubay-Cokgor E
AD  - Istanbul Technical University, Faculty of Civil Engineering, Environmental 
      Engineering Department, 34469 Maslak, Istanbul, Turkey. Electronic address: 
      ubay@itu.edu.tr.
FAU - Orhon, Derin
AU  - Orhon D
AD  - Istanbul Technical University, Faculty of Civil Engineering, Environmental 
      Engineering Department, 34469 Maslak, Istanbul, Turkey; ENVIS Energy and 
      Environmental Systems R&D Ltd, ITU Ari Teknokent, Ari 1 Building No.16, Maslak 
      34469, Turkey. Electronic address: orhon@itu.edu.tr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160514
PL  - England
TA  - Water Res
JT  - Water research
JID - 0105072
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
RN  - 7664-41-7 (Ammonia)
RN  - JE42381TNV (Sulfamethoxazole)
SB  - IM
MH  - Ammonia/metabolism
MH  - Bacteria/metabolism
MH  - Nitrification
MH  - Oxidation-Reduction
MH  - RNA, Ribosomal, 16S/*genetics
MH  - Sewage/microbiology
MH  - Sulfamethoxazole/*metabolism
OTO - NOTNLM
OT  - Community molecular analyses
OT  - Enriched nitrifying culture
OT  - Gene activity
OT  - Nitrification
OT  - Sulfamethoxazole
EDAT- 2016/05/29 06:00
MHDA- 2017/05/06 06:00
CRDT- 2016/05/29 06:00
PHST- 2016/02/08 00:00 [received]
PHST- 2016/05/12 00:00 [revised]
PHST- 2016/05/13 00:00 [accepted]
PHST- 2016/05/29 06:00 [entrez]
PHST- 2016/05/29 06:00 [pubmed]
PHST- 2017/05/06 06:00 [medline]
AID - S0043-1354(16)30370-0 [pii]
AID - 10.1016/j.watres.2016.05.043 [doi]
PST - ppublish
SO  - Water Res. 2016 Sep 1;100:546-555. doi: 10.1016/j.watres.2016.05.043. Epub 2016 
      May 14.