PMID- 27235868
OWN - NLM
STAT- MEDLINE
DCOM- 20170622
LR  - 20191210
IS  - 1872-6240 (Electronic)
IS  - 0006-8993 (Linking)
VI  - 1646
DP  - 2016 Sep 1
TI  - Hypothermia-induced ischemic tolerance is associated with Drp1 inhibition in 
      cerebral ischemia-reperfusion injury of mice.
PG  - 73-83
LID - S0006-8993(16)30404-8 [pii]
LID - 10.1016/j.brainres.2016.05.042 [doi]
AB  - Excessive mitochondrial fission activation has been implicated in cerebral 
      ischemia-reperfusion (IR) injury. Hypothermia is effective in preventing cerebral 
      ischemic damage. However, effects of hypothermia on ischemia-induced 
      mitochondrial fission activation is not well known. Therefore, the aim of this 
      study was to investigate whether hypothermia protect the brain by inhibiting 
      mitochondrial fission-related proteins activation following cerebral IR injury. 
      Adult male C57BL/6 mice were subjected to transient forebrain ischemia induced by 
      15min of bilateral common carotid artery occlusion (BCCAO). Mice were divided 
      into three groups (n=48 each): Hypothermia (HT) group, with mild hypothermia 
      (32-34 degrees C) for 4h; Normothermia (NT) group, similarly as HT group except for 
      cooling; Sham group, with vessels exposed but without occlusion or cooling. 
      Hematoxylin and eosin (HE), Nissl staining, Terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) staining and behavioral 
      testing (n=6 each) demonstrated that hypothermia significantly decreased 
      ischemia-induced neuronal injury. The expressions of Dynamin related protein 1 
      (Drp1) and Cytochrome C (Cyto C) (n=6 each) in mice hippocampus were measured at 
      3, 6, 24, and 72h of reperfusion. IR injury significantly increased expressions 
      of total Drp1, phosphorylated Drp1 (P-Drp1 S616) and Cyto C under normothermia. 
      However, mild hypothermia inhibited Drp1 activation and Cyto C cytosolic release, 
      preserved neural cells integrity and reduced neuronal necrosis and apoptosis. 
      These findings indicated that mild hypothermia-induced neuroprotective effects 
      against ischemia-reperfusion injury is associated with suppressing mitochondrial 
      fission-related proteins activation and apoptosis execution.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Tang, Yingying
AU  - Tang Y
AD  - Department of Anesthesiology, Qingdao Municipal Hospital, Dalian Medical 
      University, Dalian, China; Department of Anesthesiology, Women's Hospital, School 
      of Medicine, Zhejiang University, Xueshi Road 1, Hangzhou, Zhejiang 310006, 
      China.
FAU - Liu, Xiaojie
AU  - Liu X
AD  - Department of Anesthesiology, Qingdao Central Hospital, Shandong, China.
FAU - Zhao, Jie
AU  - Zhao J
AD  - Department of Anesthesiology, Qingdao Municipal Hospital, Dalian Medical 
      University, Dalian, China.
FAU - Tan, Xueying
AU  - Tan X
AD  - Department of Hepatobiliary Surgery, Qingdao Municipal Hospital, Shandong, China.
FAU - Liu, Bing
AU  - Liu B
AD  - Department of Anesthesiology, Qingdao Municipal Hospital, Dalian Medical 
      University, Dalian, China.
FAU - Zhang, Gaofeng
AU  - Zhang G
AD  - Department of Anesthesiology, Qingdao Municipal Hospital, School of Medicine, 
      Qingdao University, Shandong, China.
FAU - Sun, Lixin
AU  - Sun L
AD  - Department of Anesthesiology, Qingdao Municipal Hospital, School of Medicine, 
      Qingdao University, Shandong, China.
FAU - Han, Dengyang
AU  - Han D
AD  - Department of Anesthesiology, Qingdao Municipal Hospital, Dalian Medical 
      University, Dalian, China.
FAU - Chen, Huailong
AU  - Chen H
AD  - Department of Anesthesiology, Qingdao Municipal Hospital, School of Medicine, 
      Qingdao University, Shandong, China. Electronic address: chenhuailongdr@163.com.
FAU - Wang, Mingshan
AU  - Wang M
AD  - Department of Anesthesiology, Qingdao Municipal Hospital, Dalian Medical 
      University, Dalian, China; Department of Anesthesiology, Qingdao Municipal 
      Hospital, School of Medicine, Qingdao University, Shandong, China. Electronic 
      address: wangmingshandr@163.com.
LA  - eng
PT  - Journal Article
DEP - 20160526
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 9007-43-6 (Cytochromes c)
RN  - EC 3.6.5.5 (Dnm1l protein, mouse)
RN  - EC 3.6.5.5 (Dynamins)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Brain Ischemia/*metabolism/pathology/*therapy
MH  - Cytochromes c/metabolism
MH  - Dynamins/*metabolism
MH  - Hippocampus/metabolism/pathology
MH  - *Hypothermia, Induced
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mitochondria/*metabolism
MH  - *Mitochondrial Dynamics
MH  - Pyramidal Cells/pathology
MH  - Recognition, Psychology
MH  - Reperfusion Injury/pathology/therapy
OTO - NOTNLM
OT  - Apoptosis
OT  - Drp1
OT  - Hypothermia
OT  - Ischemia-reperfusion injury
OT  - Mitochondria fission
EDAT- 2016/05/29 06:00
MHDA- 2017/06/24 06:00
CRDT- 2016/05/29 06:00
PHST- 2016/02/15 00:00 [received]
PHST- 2016/04/22 00:00 [revised]
PHST- 2016/05/24 00:00 [accepted]
PHST- 2016/05/29 06:00 [entrez]
PHST- 2016/05/29 06:00 [pubmed]
PHST- 2017/06/24 06:00 [medline]
AID - S0006-8993(16)30404-8 [pii]
AID - 10.1016/j.brainres.2016.05.042 [doi]
PST - ppublish
SO  - Brain Res. 2016 Sep 1;1646:73-83. doi: 10.1016/j.brainres.2016.05.042. Epub 2016 
      May 26.