PMID- 27236795
OWN - NLM
STAT- MEDLINE
DCOM- 20171213
LR  - 20201226
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1428
DP  - 2016
TI  - Engineering WT1-Encoding mRNA to Increase Translational Efficiency in Dendritic 
      Cells.
PG  - 115-23
LID - 10.1007/978-1-4939-3625-0_7 [doi]
AB  - Dendritic cells (DCs) are the orchestrators of the immune system and are 
      frequently used in clinical trials in order to boost the immune system in cancer 
      patients. Among several available techniques for DC modification, mRNA 
      electroporation is an interesting technique due to the favorable characteristics 
      of mRNA. Antigen expression level and duration can be increased by multiple 
      optimizations of an antigen-encoding mRNA template. Here, we describe different 
      molecular modifications to a WT1-encoding mRNA construct in order to increase 
      antigen expression and the subsequent introduction of mRNA into DCs.
FAU - Benteyn, Daphne
AU  - Benteyn D
AD  - Laboratory of Molecular and Cellular Therapy, Department of Immunology-Physiology 
      and the Dendritic Cell Bank, Vrije Universiteit Brussel, Laarbeeklaan 103/E235, 
      1090, Brussels, Belgium. Daphne.benteyn@vub.ac.be.
FAU - Heirman, Carlo
AU  - Heirman C
AD  - Laboratory of Molecular and Cellular Therapy, Department of Immunology-Physiology 
      and the Dendritic Cell Bank, Vrije Universiteit Brussel, Laarbeeklaan 103/E235, 
      1090, Brussels, Belgium.
FAU - Thielemans, Kris
AU  - Thielemans K
AD  - Laboratory of Molecular and Cellular Therapy, Department of Immunology-Physiology 
      and the Dendritic Cell Bank, Vrije Universiteit Brussel, Laarbeeklaan 103/E235, 
      1090, Brussels, Belgium.
FAU - Bonehill, Aude
AU  - Bonehill A
AD  - Laboratory of Molecular and Cellular Therapy, Department of Immunology-Physiology 
      and the Dendritic Cell Bank, Vrije Universiteit Brussel, Laarbeeklaan 103/E235, 
      1090, Brussels, Belgium.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (RNA Caps)
RN  - 0 (RNA, Messenger)
RN  - 0 (WT1 Proteins)
RN  - 0 (WT1 protein, human)
SB  - IM
MH  - Dendritic Cells/*metabolism
MH  - Electroporation
MH  - Genetic Engineering/*methods
MH  - Humans
MH  - Protein Biosynthesis
MH  - RNA Caps/metabolism
MH  - RNA, Messenger/chemistry/genetics/*metabolism
MH  - WT1 Proteins/*genetics/metabolism
OTO - NOTNLM
OT  - Antigen expression
OT  - Cancer
OT  - Dendritic cells
OT  - Immunotherapy
OT  - Vaccination
OT  - Wilms' Tumor 1
OT  - mRNA
EDAT- 2016/05/30 06:00
MHDA- 2017/12/14 06:00
CRDT- 2016/05/30 06:00
PHST- 2016/05/30 06:00 [entrez]
PHST- 2016/05/30 06:00 [pubmed]
PHST- 2017/12/14 06:00 [medline]
AID - 10.1007/978-1-4939-3625-0_7 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2016;1428:115-23. doi: 10.1007/978-1-4939-3625-0_7.