PMID- 27239777
OWN - NLM
STAT- MEDLINE
DCOM- 20170118
LR  - 20181202
IS  - 1879-1514 (Electronic)
IS  - 0166-445X (Linking)
VI  - 177
DP  - 2016 Aug
TI  - Heritable oxidative phosphorylation differences in a pollutant resistant Fundulus 
      heteroclitus population.
PG  - 44-50
LID - S0166-445X(16)30132-1 [pii]
LID - 10.1016/j.aquatox.2016.05.007 [doi]
AB  - Populations can adapt to stress including recent anthropogenic pollution. Our 
      published data suggests heritable differences in hepatocyte oxidative 
      phosphorylation (OxPhos) metabolism in field-caught killifish (Fundulus 
      heteroclitus) from the highly polluted Elizabeth River, VA, USA, relative to fish 
      from a nearby, relatively unpolluted reference site in King's Creek VA. 
      Consistent with other studies showing that Elizabeth River killifish are 
      resistant to some of the toxic effects of certain contaminants, OxPhos 
      measurements in hepatocytes from field-caught King's Creek but not field-caught 
      Elizabeth River killifish were altered by acute benzo [a] pyrene exposures. To 
      more definitively test whether the enhanced OxPhos metabolism and toxicity 
      resistance are heritable, we measured OxPhos metabolism in a laboratory-reared F3 
      generation from the Elizabeth River population versus a laboratory-reared F1 
      generation from the King's Creek population and compared these results to 
      previous data from the field-caught fish. The F3 Elizabeth River fish compared to 
      F1 King's Creek fish had significantly higher State 3 respiration (routine 
      metabolism) and complex II activity, and significantly lower complex I activity. 
      The consistently higher routine metabolism in the F3 and field-caught Elizabeth 
      River fish versus F1 and field-caught King's Creek fish implies a heritable 
      change in OxPhos function. The observation that LEAK, E-State, Complex I and 
      Complex II were different in laboratory bred versus field-caught fish suggests 
      that different physiological mechanisms produce the enhanced OxPhos differences. 
      Finally, similar to field-caught Elizabeth River fish, acute benzo [a] pyrene 
      exposure did not affect OxPhos function of the laboratory-reared F3 generation, 
      supporting the heritability of the toxicity resistance. Overall, these results 
      suggest that the Elizabeth River population has evolved genetic changes in 
      physiological homeostasis that enhance routine metabolism, and we speculate that 
      these genetic changes interact with environmental factors altering the 
      physiological mechanisms (e.g., alter LEAK, Complex I, and electron transfer 
      system capacity) used to achieve this enhanced metabolism.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Du, Xiao
AU  - Du X
AD  - Marine Biology and Ecology, Rosenstiel School of Marine and Atmospheric Science, 
      University of Miami, 4600 Rickenbacker Causeway, Miami, FL 33149, USA. Electronic 
      address: xdu@rsmas.miami.edu.
FAU - Crawford, Douglas L
AU  - Crawford DL
AD  - Marine Biology and Ecology, Rosenstiel School of Marine and Atmospheric Science, 
      University of Miami, 4600 Rickenbacker Causeway, Miami, FL 33149, USA.
FAU - Nacci, Diane E
AU  - Nacci DE
AD  - Population Ecology Branch, Atlantic Ecology Division, Office of Research and 
      Development, U.S. Environmental Protection Agency, 27 Tarzwell Dr., Narragansett, 
      RI 02882, USA.
FAU - Oleksiak, Marjorie F
AU  - Oleksiak MF
AD  - Marine Biology and Ecology, Rosenstiel School of Marine and Atmospheric Science, 
      University of Miami, 4600 Rickenbacker Causeway, Miami, FL 33149, USA. Electronic 
      address: moleksiak@rsmas.miami.edu.
LA  - eng
PT  - Journal Article
DEP - 20160516
PL  - Netherlands
TA  - Aquat Toxicol
JT  - Aquatic toxicology (Amsterdam, Netherlands)
JID - 8500246
RN  - 0 (Water Pollutants, Chemical)
RN  - 3417WMA06D (Benzo(a)pyrene)
SB  - IM
MH  - Adaptation, Biological/*genetics
MH  - Animals
MH  - Benzo(a)pyrene/toxicity
MH  - Fundulidae/*genetics/*metabolism
MH  - Hepatocytes/metabolism
MH  - *Oxidative Phosphorylation
MH  - Quantitative Trait, Heritable
MH  - Rivers/chemistry
MH  - Water Pollutants, Chemical/toxicity
OTO - NOTNLM
OT  - Benzo [a] pyrene
OT  - Elizabeth river
OT  - F3 generation
OT  - Fundulus heteroclitus
OT  - Oxidative phosphorylation
EDAT- 2016/05/31 06:00
MHDA- 2017/01/19 06:00
CRDT- 2016/05/31 06:00
PHST- 2016/02/24 00:00 [received]
PHST- 2016/05/13 00:00 [revised]
PHST- 2016/05/14 00:00 [accepted]
PHST- 2016/05/31 06:00 [entrez]
PHST- 2016/05/31 06:00 [pubmed]
PHST- 2017/01/19 06:00 [medline]
AID - S0166-445X(16)30132-1 [pii]
AID - 10.1016/j.aquatox.2016.05.007 [doi]
PST - ppublish
SO  - Aquat Toxicol. 2016 Aug;177:44-50. doi: 10.1016/j.aquatox.2016.05.007. Epub 2016 
      May 16.