PMID- 27242759
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160531
LR  - 20200929
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 7
DP  - 2016
TI  - Effects of Incubation Conditions on Cr(VI) Reduction by c-type Cytochromes in 
      Intact Shewanella oneidensis MR-1 Cells.
PG  - 746
LID - 10.3389/fmicb.2016.00746 [doi]
LID - 746
AB  - It is widely recognized that the outer membrane c-type cytochromes (OM c-Cyts) of 
      metal-reducing bacteria play a key role in microbial metal reduction processes. 
      However, the in situ redox status of OM c-Cyts during microbial metal reduction 
      processes remain poorly understood. In this study, diffuse-transmission UV/Vis 
      spectroscopy is used to investigate the in situ spectral reaction of Cr(VI) 
      reduction by c-Cyts in intact Shewanella oneidensis MR-1 cells under different 
      incubation conditions. The reduced c-Cyts decreased transiently at the beginning 
      and then recovered gradually over time. The Cr(VI) reduction rates decreased with 
      increasing initial Cr(VI) concentrations, and Cr(III) was identified as a reduced 
      product. The presence of Cr(III) substantially inhibited Cr(VI) reduction and the 
      recovery of reduced c-Cyts, indicating that Cr(III) might inhibit cell growth. 
      Cr(VI) reduction rates increased with increasing cell density. The highest Cr(VI) 
      reduction rate and fastest recovery of c-Cyts were obtained at pH 7.0 and 30 degrees C, 
      with sodium lactate serving as an electron donor. The presence of O2 strongly 
      inhibited Cr(VI) reduction, suggesting that O2 might compete with Cr(VI) as an 
      electron acceptor in cells. This study provides a case of directly examining in 
      vivo reaction properties of an outer-membrane enzyme during microbial metal 
      reduction processes under non-invasive physiological conditions.
FAU - Han, Rui
AU  - Han R
AD  - School of Environment and Energy, South China University of TechnologyGuangzhou, 
      China; Guangdong Key Laboratory of Agricultural Environment Pollution Integrated 
      Control, Guangdong Institute of Eco-Environmental and Soil SciencesGuangzhou, 
      China.
FAU - Li, Fangbai
AU  - Li F
AD  - Guangdong Key Laboratory of Agricultural Environment Pollution Integrated 
      Control, Guangdong Institute of Eco-Environmental and Soil Sciences Guangzhou, 
      China.
FAU - Liu, Tongxu
AU  - Liu T
AD  - Guangdong Key Laboratory of Agricultural Environment Pollution Integrated 
      Control, Guangdong Institute of Eco-Environmental and Soil Sciences Guangzhou, 
      China.
FAU - Li, Xiaomin
AU  - Li X
AD  - Guangdong Key Laboratory of Agricultural Environment Pollution Integrated 
      Control, Guangdong Institute of Eco-Environmental and Soil Sciences Guangzhou, 
      China.
FAU - Wu, Yundang
AU  - Wu Y
AD  - Guangdong Key Laboratory of Agricultural Environment Pollution Integrated 
      Control, Guangdong Institute of Eco-Environmental and Soil Sciences Guangzhou, 
      China.
FAU - Wang, Ying
AU  - Wang Y
AD  - Guangdong Key Laboratory of Agricultural Environment Pollution Integrated 
      Control, Guangdong Institute of Eco-Environmental and Soil Sciences Guangzhou, 
      China.
FAU - Chen, Dandan
AU  - Chen D
AD  - Guangdong Key Laboratory of Agricultural Environment Pollution Integrated 
      Control, Guangdong Institute of Eco-Environmental and Soil Sciences Guangzhou, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20160519
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC4872037
OTO - NOTNLM
OT  - Cr(VI) reduction
OT  - Shewanella oneidensis MR-1
OT  - c-type cytochromes
OT  - incubation conditions
OT  - intact cells
EDAT- 2016/06/01 06:00
MHDA- 2016/06/01 06:01
PMCR- 2016/05/19
CRDT- 2016/06/01 06:00
PHST- 2015/10/02 00:00 [received]
PHST- 2016/05/03 00:00 [accepted]
PHST- 2016/06/01 06:00 [entrez]
PHST- 2016/06/01 06:00 [pubmed]
PHST- 2016/06/01 06:01 [medline]
PHST- 2016/05/19 00:00 [pmc-release]
AID - 10.3389/fmicb.2016.00746 [doi]
PST - epublish
SO  - Front Microbiol. 2016 May 19;7:746. doi: 10.3389/fmicb.2016.00746. eCollection 
      2016.