PMID- 27245201
OWN - NLM
STAT- MEDLINE
DCOM- 20170306
LR  - 20170306
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Linking)
VI  - 38
IP  - 1
DP  - 2016 Jul
TI  - Network analysis of genes involved in the enhancement of hyperthermia sensitivity 
      by the knockdown of BAG3 in human oral squamous cell carcinoma cells.
PG  - 236-42
LID - 10.3892/ijmm.2016.2621 [doi]
AB  - BCL2-associated athanogene 3 (BAG3), a co-chaperone of the heat shock 70 kDa 
      protein (HSPA) family of proteins, is a cytoprotective protein that acts against 
      various stresses, including heat stress. The aim of the present study was to 
      identify gene networks involved in the enhancement of hyperthermia (HT) 
      sensitivity by the knockdown (KD) of BAG3 in human oral squamous cell 
      carcinoma (OSCC) cells. Although a marked elevation in the protein expression of 
      BAG3 was detected in human the OSCC HSC-3 cells exposed to HT at 44 C for 90 min, 
      its expression was almost completely suppressed in the cells transfected with 
      small interfering RNA against BAG3 (siBAG) under normal and HT conditions. The 
      silencing of BAG3 also enhanced the cell death that was increased in the 
      HSC-3 cells by exposure to HT. Global gene expression analysis revealed many 
      genes that were differentially expressed by >2-fold in the cells exposed to HT 
      and transfected with siBAG. Moreover, Ingenuity(R) pathways analysis demonstrated 
      two unique gene networks, designated as Pro-cell death and Anti-cell death, which 
      were obtained from upregulated genes and were mainly associated with the 
      biological functions of induction and the prevention of cell death, respectively. 
      Of note, the expression levels of genes in the Pro-cell death and Anti-cell death 
      gene networks were significantly elevated and reduced in the HT + BAG3-KD group 
      compared to those in the HT control group, respectively. These results provide 
      further insight into the molecular mechanisms involved in the enhancement of 
      HT sensitivity by the silencing of BAG3 in human OSCC cells.
FAU - Yunoki, Tatsuya
AU  - Yunoki T
AD  - Department of Ophthalmology, Graduate School of Medicine and Pharmaceutical 
      Sciences, University of Toyama, Toyama 930-0194, Japan.
FAU - Tabuchi, Yoshiaki
AU  - Tabuchi Y
AD  - Division of Molecular Genetics Research, Life Science Research Center, University 
      of Toyama, Toyama 930-0194, Japan.
FAU - Hayashi, Atsushi
AU  - Hayashi A
AD  - Department of Ophthalmology, Graduate School of Medicine and Pharmaceutical 
      Sciences, University of Toyama, Toyama 930-0194, Japan.
FAU - Kondo, Takashi
AU  - Kondo T
AD  - Department of Radiological Sciences, Graduate School of Medicine and 
      Pharmaceutical Sciences, University of Toyama, Toyama 930-0194, Japan.
LA  - eng
PT  - Journal Article
DEP - 20160531
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (BAG3 protein, human)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/*genetics/metabolism
MH  - Apoptosis Regulatory Proteins/*genetics/metabolism
MH  - Carcinoma, Squamous Cell/*genetics
MH  - Cell Death
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Gene Expression Profiling
MH  - *Gene Expression Regulation, Neoplastic
MH  - *Gene Knockdown Techniques
MH  - *Gene Regulatory Networks
MH  - Humans
MH  - *Hyperthermia, Induced
MH  - Mouth Neoplasms/*genetics
MH  - RNA, Messenger/genetics/metabolism
EDAT- 2016/06/02 06:00
MHDA- 2017/03/07 06:00
CRDT- 2016/06/02 06:00
PHST- 2015/11/25 00:00 [received]
PHST- 2016/05/16 00:00 [accepted]
PHST- 2016/06/02 06:00 [entrez]
PHST- 2016/06/02 06:00 [pubmed]
PHST- 2017/03/07 06:00 [medline]
AID - 10.3892/ijmm.2016.2621 [doi]
PST - ppublish
SO  - Int J Mol Med. 2016 Jul;38(1):236-42. doi: 10.3892/ijmm.2016.2621. Epub 2016 May 
      31.