PMID- 27246033
OWN - NLM
STAT- MEDLINE
DCOM- 20171213
LR  - 20180316
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1422
DP  - 2016
TI  - Isolation and Functional Analysis of Lamina Propria Dendritic Cells from the 
      Mouse Small Intestine.
PG  - 181-8
LID - 10.1007/978-1-4939-3603-8_17 [doi]
AB  - Dendritic cells (DCs) are the most professional antigen-presenting cells that are 
      indispensable for the initiation of adaptive immune responses. DCs are 
      heterogeneous in terms of their origin, anatomical location, cell-surface 
      markers, and functions. Previous studies have demonstrated that there exist 
      several groups of DCs in the lamina propria (LPDC) of gastrointestinal tract, 
      which collectively contribute to the maintenance of gut homeostasis through the 
      regulation of the balance between active immunity and tolerance. However, 
      although intestinal LPDCs are attractive research target for understanding the 
      immunological mechanisms in the gut, isolation of the LPDCs is complicated and 
      technically difficult for unskilled people. Therefore, establishment of the 
      method to isolate intestinal LPDCs is a major obstacle in this research. Here, we 
      describe the methods that we have established for the isolation of primary DCs 
      from the LP of mouse small intestine. Our isolation method provides high yield of 
      viable LP leukocytes (LPLs) including DCs. Combination with FACS sorting allows 
      for the selective isolation of CD103(+)CD8alpha(+) DCs and CD103(+)CD8alpha(-) DCs from 
      the LPLs. Furthermore, isolated LPDCs can be subjected to immunological assays, 
      such as measurement of cytokine productions following stimulation of Toll-like 
      receptors. Thus, our methods would be useful for studying the functions of LPDCs 
      of mouse small intestine.
FAU - Takemura, Naoki
AU  - Takemura N
AD  - Department of Mucosal Immunology, School of Medicine, Chiba University, 1-8-1 
      Inohana, Chuou-ku, Chiba, 260-8670, Japan.
AD  - Division of Innate Immune Regulation, International Research and Development 
      Center for Mucosal Vaccines, Institute of Medical Science, The University of 
      Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo, 108-8639, Japan.
FAU - Uematsu, Satoshi
AU  - Uematsu S
AD  - Department of Mucosal Immunology, School of Medicine, Chiba University, 1-8-1 
      Inohana, Chuou-ku, Chiba, 260-8670, Japan. suematsu@ims.u-tokyo.ac.jp.
AD  - Division of Innate Immune Regulation, International Research and Development 
      Center for Mucosal Vaccines, Institute of Medical Science, The University of 
      Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo, 108-8639, Japan. 
      suematsu@ims.u-tokyo.ac.jp.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Animals
MH  - Cell Separation/*methods
MH  - Dendritic Cells/*cytology
MH  - Flow Cytometry/methods
MH  - Intestinal Mucosa/cytology
MH  - Intestine, Small/*cytology
MH  - Mice
MH  - Mucous Membrane/cytology
OTO - NOTNLM
OT  - CD103
OT  - CD11b
OT  - CD11c
OT  - CD8alpha
OT  - Dendritic cell
OT  - Flow cytometry
OT  - Lamina propria
OT  - Mouse small intestine
OT  - Toll-like receptor
EDAT- 2016/06/02 06:00
MHDA- 2017/12/14 06:00
CRDT- 2016/06/02 06:00
PHST- 2016/06/02 06:00 [entrez]
PHST- 2016/06/02 06:00 [pubmed]
PHST- 2017/12/14 06:00 [medline]
AID - 10.1007/978-1-4939-3603-8_17 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2016;1422:181-8. doi: 10.1007/978-1-4939-3603-8_17.