PMID- 27246444
OWN - NLM
STAT- MEDLINE
DCOM- 20170522
LR  - 20181113
IS  - 1476-1645 (Electronic)
IS  - 0002-9637 (Print)
IS  - 0002-9637 (Linking)
VI  - 95
IP  - 2
DP  - 2016 Aug 3
TI  - Enteroviruses and Rhinoviruses: Molecular Epidemiology of the Most Influenza-Like 
      Illness Associated Viruses in Senegal.
PG  - 339-47
LID - 10.4269/ajtmh.15-0799 [doi]
AB  - Different viruses have been identified as etiologic agents of respiratory tract 
      infections, including severe cases. Among these, human rhinoviruses (HRVs) and 
      human enteroviruses (HEVs) are recognized as leading causes. The present study 
      describes the molecular epidemiology of HRVs and HEVs in Senegal over a 3-year 
      surveillance period. From January 2012 to December 2014, nasopharyngeal and 
      oropharyngeal swabs specimen were collected from patients with influenza-like 
      illness (ILI). A real-time reverse transcription polymerase chain reaction was 
      performed for HRV and HEV detection using the RV16 kit. Two regions were targeted 
      for the molecular characterization of RVs: 5' untranslated region (5'UTR) and 
      viral protein 4/viral protein 2 (VP4/VP2) transition region. For enteroviruses 
      (EVs) phylogeny, VP1 gene was targeted. A total of 4,194 samples were collected. 
      Children up to 5 years accounted for 52.9%. Among them, 1,415 (33.7%) were 
      positive for HRV, 857 (20.4%) for HEV, and 437 cases of dual infections HRV/HEV. 
      HRVs and HEVs were identified significantly in children aged 5 years or less. 
      Only cough and vomiting signs were observed with significant association with 
      viral infection. Both viruses co-circulated all year long with a marked increase 
      of activity during rainy and cold period. All HRV types circulate in Senegal. 
      HRV-A and C groups were the most common. HEV serotyping identified coxsackie B 
      viruses (CBV) only. VP1 region revealed different CBV (CBV1, CBV2, CBV3, CBV4, 
      and CBV5), echoviruses, coxsackieviruses A4-like strains and a poliovirus 2. The 
      results suggest strong year-round respiratory picornavirus activity in children 
      up to 5 years of age. Molecular studies identified a wide variety of RVs along 
      with diverse EVs in samples from patients with ILI.
CI  - (c) The American Society of Tropical Medicine and Hygiene.
FAU - Fall, Amary
AU  - Fall A
AD  - Unite de Virologie Medicale, Institut Pasteur de Dakar, Dakar, Senegal.
FAU - Dia, Ndongo
AU  - Dia N
AD  - Unite de Virologie Medicale, Institut Pasteur de Dakar, Dakar, Senegal.
FAU - Kebe, Ousmane
AU  - Kebe O
AD  - Unite de Virologie Medicale, Institut Pasteur de Dakar, Dakar, Senegal.
FAU - Sarr, Fatoumata Diene
AU  - Sarr FD
AD  - Unite d'Epidemiologie des Maladies Infectieuses, Institut Pasteur de Dakar, 
      Dakar, Senegal.
FAU - Kiori, Davy E
AU  - Kiori DE
AD  - Unite de Virologie Medicale, Institut Pasteur de Dakar, Dakar, Senegal.
FAU - Cisse, El Hadj Abdoul Khadir
AU  - Cisse el HA
AD  - Unite de Virologie Medicale, Institut Pasteur de Dakar, Dakar, Senegal.
FAU - Sy, Sara
AU  - Sy S
AD  - Unite de Virologie Medicale, Institut Pasteur de Dakar, Dakar, Senegal.
FAU - Goudiaby, Deborah
AU  - Goudiaby D
AD  - Unite de Virologie Medicale, Institut Pasteur de Dakar, Dakar, Senegal.
FAU - Richard, Vincent
AU  - Richard V
AD  - Unite d'Epidemiologie des Maladies Infectieuses, Institut Pasteur de Dakar, 
      Dakar, Senegal.
FAU - Diop, Ousmane Madiagne
AU  - Diop OM
AD  - Unite de Virologie Medicale, Institut Pasteur de Dakar, Dakar, Senegal.
FAU - Niang, Mbayame Ndiaye
AU  - Niang MN
AD  - Unite de Virologie Medicale, Institut Pasteur de Dakar, Dakar, Senegal. 
      niang@pasteur.sn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160531
PL  - United States
TA  - Am J Trop Med Hyg
JT  - The American journal of tropical medicine and hygiene
JID - 0370507
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Capsid Proteins)
RN  - 0 (VP2 protein, Rhinovirus)
RN  - 0 (Viral Proteins)
RN  - 0 (viral protein 1, rhinovirus)
SB  - IM
MH  - 5' Untranslated Regions/genetics
MH  - Adolescent
MH  - Adult
MH  - Capsid Proteins/genetics
MH  - Child
MH  - Child, Preschool
MH  - Enterovirus B, Human/chemistry/*genetics/isolation & purification
MH  - Enterovirus Infections/diagnosis/*epidemiology/virology
MH  - Female
MH  - Humans
MH  - Incidence
MH  - Infant
MH  - Infant, Newborn
MH  - Male
MH  - Middle Aged
MH  - Molecular Epidemiology
MH  - Nasopharynx/virology
MH  - *Phylogeny
MH  - Picornaviridae Infections/diagnosis/*epidemiology/virology
MH  - Public Health Surveillance
MH  - Real-Time Polymerase Chain Reaction
MH  - Respiratory Tract Infections/diagnosis/*epidemiology/virology
MH  - Rhinovirus/chemistry/*genetics/isolation & purification
MH  - Senegal/epidemiology
MH  - Viral Proteins/genetics
PMC - PMC4973179
EDAT- 2016/06/02 06:00
MHDA- 2017/05/23 06:00
PMCR- 2017/08/03
CRDT- 2016/06/02 06:00
PHST- 2015/11/06 00:00 [received]
PHST- 2016/04/04 00:00 [accepted]
PHST- 2016/06/02 06:00 [entrez]
PHST- 2016/06/02 06:00 [pubmed]
PHST- 2017/05/23 06:00 [medline]
PHST- 2017/08/03 00:00 [pmc-release]
AID - ajtmh.15-0799 [pii]
AID - 10.4269/ajtmh.15-0799 [doi]
PST - ppublish
SO  - Am J Trop Med Hyg. 2016 Aug 3;95(2):339-47. doi: 10.4269/ajtmh.15-0799. Epub 2016 
      May 31.