PMID- 27247346
OWN - NLM
STAT- MEDLINE
DCOM- 20170824
LR  - 20210217
IS  - 1539-7262 (Electronic)
IS  - 0022-2275 (Print)
IS  - 0022-2275 (Linking)
VI  - 57
IP  - 7
DP  - 2016 Jul
TI  - Effects of dietary carbohydrate on hepatic de novo lipogenesis in European 
      seabass (Dicentrarchus labrax L.).
PG  - 1264-72
LID - 10.1194/jlr.M067850 [doi]
AB  - Farmed seabass have higher adiposity than their wild counterparts and this is 
      often attributed to carbohydrate (CHO) feeding. Whether this reflects a reduction 
      in fat oxidation, increased de novo lipogenesis (DNL), or both, is not known. To 
      study the effects of high CHO diets on hepatic TG biosynthesis, hepatic TG 
      deuterium ((2)H) enrichment was determined following 6 days in (2)H-enriched tank 
      water for fish fed with a no-CHO control diet (CTRL), and diets with digestible 
      starch (DS) and raw starch (RS). Hepatic fractional synthetic rates (FSRs, 
      percent per day(-1)) were calculated for hepatic TG-glyceryl and FA moieties 
      through (2)H NMR analysis. Glyceryl FSRs exceeded FA FSRs in all cases, 
      indicating active cycling. DS fish did not show increased lipogenic potential 
      compared to CTRL. RS fish had lower glyceryl FSRs compared with the other diets 
      and negligible levels of FA FSRs despite similar hepatic TG levels to CTRL. 
      DS-fed fish showed higher activity for enzymes that can provide NADPH for 
      lipogenesis, relative to CTRL in the case of glucose-6-phosphate dehydrogenase 
      (G6PDH) and relative to RS for both G6PDH and 6-phosphogluconate dehydrogenase. 
      This approach indicated that elevated hepatic adiposity from DS feeding was not 
      attributable to increased DNL.
CI  - Copyright (c) 2016 by the American Society for Biochemistry and Molecular Biology, 
      Inc.
FAU - Viegas, Ivan
AU  - Viegas I
AUID- ORCID: 0000-0003-2589-2212
AD  - Center for Neuroscience and Cell Biology, University of Coimbra, 3004-517 
      Coimbra, Portugal Center for Functional Ecology, Department Life Sciences, 
      University of Coimbra, 3000-456 Coimbra, Portugal iviegas@ci.uc.pt.
FAU - Jarak, Ivana
AU  - Jarak I
AD  - Center for Neuroscience and Cell Biology, University of Coimbra, 3004-517 
      Coimbra, Portugal.
FAU - Rito, Joao
AU  - Rito J
AD  - Center for Neuroscience and Cell Biology, University of Coimbra, 3004-517 
      Coimbra, Portugal Center for Functional Ecology, Department Life Sciences, 
      University of Coimbra, 3000-456 Coimbra, Portugal.
FAU - Carvalho, Rui A
AU  - Carvalho RA
AD  - Center for Functional Ecology, Department Life Sciences, University of Coimbra, 
      3000-456 Coimbra, Portugal.
FAU - Meton, Isidoro
AU  - Meton I
AD  - Seccio de Bioquimica i Biologia Molecular, Departament de Bioquimica i 
      Fisiologia, Facultat de Farmacia, Universitat de Barcelona, 08028 Barcelona, 
      Spain.
FAU - Pardal, Miguel A
AU  - Pardal MA
AD  - Center for Functional Ecology, Department Life Sciences, University of Coimbra, 
      3000-456 Coimbra, Portugal.
FAU - Baanante, Isabel V
AU  - Baanante IV
AD  - Seccio de Bioquimica i Biologia Molecular, Departament de Bioquimica i 
      Fisiologia, Facultat de Farmacia, Universitat de Barcelona, 08028 Barcelona, 
      Spain.
FAU - Jones, John G
AU  - Jones JG
AD  - Center for Neuroscience and Cell Biology, University of Coimbra, 3004-517 
      Coimbra, Portugal.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160531
PL  - United States
TA  - J Lipid Res
JT  - Journal of lipid research
JID - 0376606
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Dietary Fats)
RN  - 0 (Triglycerides)
SB  - IM
MH  - Animals
MH  - Bass/*metabolism
MH  - Dietary Carbohydrates/administration & dosage
MH  - Dietary Fats/administration & dosage
MH  - Lipogenesis/drug effects/*physiology
MH  - Liver/drug effects/*metabolism
MH  - Triglycerides/*metabolism
PMC - PMC4918855
OTO - NOTNLM
OT  - carbohydrate metabolism
OT  - dehydrogenases
OT  - deuterated water
OT  - fish aquaculture
OT  - lipolysis and fatty acid metabolism
OT  - liver
OT  - nuclear magnetic resonance
OT  - nutrition/carbohydrate
EDAT- 2016/06/02 06:00
MHDA- 2017/08/25 06:00
PMCR- 2017/07/01
CRDT- 2016/06/02 06:00
PHST- 2016/03/22 00:00 [received]
PHST- 2016/06/02 06:00 [entrez]
PHST- 2016/06/02 06:00 [pubmed]
PHST- 2017/08/25 06:00 [medline]
PHST- 2017/07/01 00:00 [pmc-release]
AID - S0022-2275(20)35162-2 [pii]
AID - m067850 [pii]
AID - 10.1194/jlr.M067850 [doi]
PST - ppublish
SO  - J Lipid Res. 2016 Jul;57(7):1264-72. doi: 10.1194/jlr.M067850. Epub 2016 May 31.