PMID- 27248979
OWN - NLM
STAT- MEDLINE
DCOM- 20170718
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 11
IP  - 6
DP  - 2016
TI  - Enhancement of Wound Healing by Non-Thermal N2/Ar Micro-Plasma Exposure in Mice 
      with Fractional-CO2-Laser-Induced Wounds.
PG  - e0156699
LID - 10.1371/journal.pone.0156699 [doi]
LID - e0156699
AB  - Micro-plasma is a possible alternative treatment for wound management. The effect 
      of micro-plasma on wound healing depends on its composition and temperature. The 
      authors previously developed a capillary-tube-based micro-plasma system that can 
      generate micro-plasma with a high nitric oxide-containing species composition and 
      mild working temperature. Here, the efficacy of micro-plasma treatment on wound 
      healing in a laser-induced skin wound mouse model was investigated. A partial 
      thickness wound was created in the back skin of each mouse and then treated with 
      micro-plasma. Non-invasive methods, namely wound closure kinetics, optical 
      coherence tomography (OCT), and laser Doppler scanning, were used to measure the 
      healing efficiency in the wound area. Neo-tissue growth and the expressions of 
      matrix metallopeptidase-3 (MMP-3) and laminin in the wound area were assessed 
      using histological and immunohistochemistry (IHC) analysis. The results show that 
      micro-plasma treatment promoted wound healing. Micro-plasma treatment 
      significantly reduced the wound bed region. The OCT images and histological 
      analysis indicates more pronounced tissue regrowth in the wound bed region after 
      micro-plasma treatment. The laser Doppler images shows that micro-plasma 
      treatment promoted blood flow in the wound bed region. The IHC results show that 
      the level of laminin increased in the wound bed region after micro-plasma 
      treatment, whereas the level of MMP-3 decreased. Based on these results, 
      micro-plasma has potential to be used to promote the healing of skin wounds 
      clinically.
FAU - Shao, Pei-Lin
AU  - Shao PL
AD  - Department of Materials Science and Engineering, National Cheng Kung University, 
      Tainan 70101, Taiwan.
FAU - Liao, Jiunn-Der
AU  - Liao JD
AD  - Department of Materials Science and Engineering, National Cheng Kung University, 
      Tainan 70101, Taiwan.
AD  - Medical Device Innovation Center, National Cheng Kung University, Tainan 70101, 
      Taiwan.
FAU - Wong, Tak-Wah
AU  - Wong TW
AD  - Department of Dermatology, Department of Biochemistry and Molecular Biology, 
      Medical College and Hospital, National Cheng Kung University, Tainan 70101, 
      Taiwan.
FAU - Wang, Yi-Cheng
AU  - Wang YC
AD  - Department of Materials Science and Engineering, National Cheng Kung University, 
      Tainan 70101, Taiwan.
FAU - Leu, Steve
AU  - Leu S
AD  - Center for Translational Research in Biomedical Sciences, Kaohsiung Chang Gung 
      Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 83301, 
      Taiwan.
FAU - Yip, Hon-Kan
AU  - Yip HK
AD  - Center for Translational Research in Biomedical Sciences, Kaohsiung Chang Gung 
      Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 83301, 
      Taiwan.
AD  - Division of Cardiology, Department of Internal Medicine, Kaohsiung Chang Gung 
      Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 83301, 
      Taiwan.
LA  - eng
PT  - Journal Article
DEP - 20160601
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 142M471B3J (Carbon Dioxide)
RN  - 67XQY1V3KH (Argon)
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - Animals
MH  - Argon
MH  - Carbon Dioxide/*administration & dosage
MH  - *Lasers
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Nitrogen
MH  - *Wound Healing
MH  - Wounds and Injuries/*etiology
PMC - PMC4889145
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2016/06/02 06:00
MHDA- 2017/07/19 06:00
PMCR- 2016/06/01
CRDT- 2016/06/02 06:00
PHST- 2016/01/25 00:00 [received]
PHST- 2016/05/18 00:00 [accepted]
PHST- 2016/06/02 06:00 [entrez]
PHST- 2016/06/02 06:00 [pubmed]
PHST- 2017/07/19 06:00 [medline]
PHST- 2016/06/01 00:00 [pmc-release]
AID - PONE-D-16-03549 [pii]
AID - 10.1371/journal.pone.0156699 [doi]
PST - epublish
SO  - PLoS One. 2016 Jun 1;11(6):e0156699. doi: 10.1371/journal.pone.0156699. 
      eCollection 2016.