PMID- 27251424
OWN - NLM
STAT- MEDLINE
DCOM- 20180424
LR  - 20181113
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 6
DP  - 2016 Jun 2
TI  - miR-200 family promotes podocyte differentiation through repression of RSAD2.
PG  - 27105
LID - 10.1038/srep27105 [doi]
LID - 27105
AB  - Mature podocytes are highly differentiated cells with several characteristic 
      phenotypic features that are involved in the glomerular filtration function. 
      During kidney development, a series of changes of the morphological 
      characteristics and cellular functions may happen in podocytes. The miR-200 
      family functions in various biological and pathological processes. But the 
      underlying molecular mechanisms of miR-200 family that functions in podocyte 
      differentiation remain poorly understood. Herein is shown that miR-200a, miR-200b 
      and miR-429 are significantly upregulated during the differentiation of 
      podocytes, with highest upregulation of miR-200a. In these cells, restraint of 
      miR-200 family by RNA interference assay revealed a prominent inhibition of cell 
      differentiation. More intriguingly, miR-200 family directly inhibited the radical 
      S-adenosyl methionine domain-containing protein 2 (RASD2) expression. Moreover, 
      further upregulation of RSAD2 combining with restraint of miR-200 family revealed 
      a promotion of podocyte dedifferentiation and proliferation. In addition, the 
      expression of RSAD2 is consistent with that of in vitro podocyte differentiation 
      in prenatal and postnatal mouse kidney, and significantly down-regulated during 
      the kidney development. Together, these findings indicate that miR-200 family may 
      potentially promote podocyte differentiation through repression of RSAD2 
      expression. Our data also demonstrate a novel role of the antiviral protein RSAD2 
      as a regulator in cell differentiation.
FAU - Li, Zhigui
AU  - Li Z
AD  - College of Medicine, State Key Laboratory of Medicinal Chemical Biology, Tianjin 
      Key Laboratory of Tumor Microenvironment and Neurovascular Regulation, Nankai 
      University, Tianjin 300071, China.
FAU - Yin, Hongqiang
AU  - Yin H
AD  - College of Medicine, State Key Laboratory of Medicinal Chemical Biology, Tianjin 
      Key Laboratory of Tumor Microenvironment and Neurovascular Regulation, Nankai 
      University, Tianjin 300071, China.
FAU - Hao, Shuang
AU  - Hao S
AD  - College of Medicine, State Key Laboratory of Medicinal Chemical Biology, Tianjin 
      Key Laboratory of Tumor Microenvironment and Neurovascular Regulation, Nankai 
      University, Tianjin 300071, China.
FAU - Wang, Lifeng
AU  - Wang L
AD  - College of Medicine, State Key Laboratory of Medicinal Chemical Biology, Tianjin 
      Key Laboratory of Tumor Microenvironment and Neurovascular Regulation, Nankai 
      University, Tianjin 300071, China.
FAU - Gao, Jing
AU  - Gao J
AD  - College of Medicine, State Key Laboratory of Medicinal Chemical Biology, Tianjin 
      Key Laboratory of Tumor Microenvironment and Neurovascular Regulation, Nankai 
      University, Tianjin 300071, China.
FAU - Tan, Xiaoyue
AU  - Tan X
AD  - College of Medicine, State Key Laboratory of Medicinal Chemical Biology, Tianjin 
      Key Laboratory of Tumor Microenvironment and Neurovascular Regulation, Nankai 
      University, Tianjin 300071, China.
FAU - Yang, Zhuo
AU  - Yang Z
AD  - College of Medicine, State Key Laboratory of Medicinal Chemical Biology, Tianjin 
      Key Laboratory of Tumor Microenvironment and Neurovascular Regulation, Nankai 
      University, Tianjin 300071, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160602
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (3' Untranslated Regions)
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn200 microRNA, mouse)
RN  - 0 (Proteins)
RN  - 0 (Rsad2 protein, mouse)
SB  - IM
MH  - 3' Untranslated Regions
MH  - Animals
MH  - Apoptosis
MH  - Base Sequence
MH  - Binding Sites
MH  - *Cell Differentiation
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Gene Expression
MH  - Kidney Cortex/metabolism
MH  - Mice
MH  - MicroRNAs/*physiology
MH  - Podocytes/*physiology
MH  - Proteins/*genetics/metabolism
MH  - RNA Interference
MH  - Up-Regulation
PMC - PMC4890021
EDAT- 2016/06/03 06:00
MHDA- 2018/04/25 06:00
PMCR- 2016/06/02
CRDT- 2016/06/03 06:00
PHST- 2016/02/23 00:00 [received]
PHST- 2016/05/11 00:00 [accepted]
PHST- 2016/06/03 06:00 [entrez]
PHST- 2016/06/03 06:00 [pubmed]
PHST- 2018/04/25 06:00 [medline]
PHST- 2016/06/02 00:00 [pmc-release]
AID - srep27105 [pii]
AID - 10.1038/srep27105 [doi]
PST - epublish
SO  - Sci Rep. 2016 Jun 2;6:27105. doi: 10.1038/srep27105.