PMID- 27252698
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160602
LR  - 20200929
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 7
DP  - 2016
TI  - The Effect of Helicobacter pylori Infection, Aging, and Consumption of Proton 
      Pump Inhibitor on Fungal Colonization in the Stomach of Dyspeptic Patients.
PG  - 801
LID - 10.3389/fmicb.2016.00801 [doi]
LID - 801
AB  - BACKGROUND: The importance of coinfection of Helicobacter pylori (H.pylori) and 
      Candida albicans (C. albicans) in the development of gastric diseases is not 
      known. In this study, the frequency of concurrent infection of H. pylori and C. 
      albicans in dyspeptic patients was assessed while considering age, gender, and 
      PPI consumption of patients. METHODS: Gastric biopsies were taken from 74 
      yeast-positive dyspeptic patients and gastric disease, age, gender, and proton 
      pump inhibitor (PPI) consumption of subjects were recorded. One antral biopsy was 
      used for rapid urease test (RUT) and one for H. pylori and yeast cultivation and 
      smear preparation. Bacterial isolates were identified according to spiral 
      morphology and the biochemical characteristics. Yeast isolates were identified on 
      Chromagar and by the Nested-PCR amplification of C. albicans-specific 
      topoisomerase II gene. Twenty-seven biopsy smears were Gram-stained and examined 
      by the light microscope for observing H. pylori and yeast cells. RESULTS: 
      Fifty-four (73%) of patients were >40 year. Of 68 patients with PPI consumption 
      record, 46 (67.6%) consumed PPI (p = 0). Comparison of patients in peptic ulcer 
      group (12, 16.2%) with (6, 8.1%) or without (6, 8.1%) H. pylori or in gastritis 
      group (62, 83.8%) with (25, 33.8%) or without (37, 50%) H. pylori showed no 
      significant difference (p > 0.05). Of the 46 patients who consumed PPI, 13 
      (17.5%) were H. pylori-positive and 33 (44.6%) H. pylori-negative (p = 0). Ten 
      out of twenty-seven smears showed the occurrence of H. pylori cells, including 
      three with yeast cells. Of the 17 H. pylori-negative smears, three showed the 
      occurrence of yeast cells only. Yeasts stained Gram-positive or Gram-negative and 
      appeared as single or budding cells. CONCLUSION: The older age and PPI 
      consumption could favor fungal colonization in the human stomach. The occurrence 
      of a considerable number of H. pylori-positive or H. pylori-negative patients 
      with gastritis or peptic ulcer shows that co-infection of Candida and H. pylori 
      or infection of yeast alone could be associated with dyspeptic diseases. The 
      occurrence of yeast cells in gastric biopsies with different Gram's reactions 
      indicates that fungi might change their cell wall components for establishing a 
      persistent colonization in the stomach.
FAU - Massarrat, Sadegh
AU  - Massarrat S
AD  - Endoscopy Department, Digestive Disease Research Institute, Tehran University of 
      Medical Sciences Tehran, Iran.
FAU - Saniee, Parastoo
AU  - Saniee P
AD  - Department of Microbiology, School of Biology, University College of Sciences, 
      University of Tehran Tehran, Iran.
FAU - Siavoshi, Farideh
AU  - Siavoshi F
AD  - Department of Microbiology, School of Biology, University College of Sciences, 
      University of Tehran Tehran, Iran.
FAU - Mokhtari, Reyhane
AU  - Mokhtari R
AD  - Department of Microbiology, School of Biology, University College of Sciences, 
      University of Tehran Tehran, Iran.
FAU - Mansour-Ghanaei, Fariborz
AU  - Mansour-Ghanaei F
AD  - Gastrointestinal and Liver Diseases Research Center, Guilan University of Medical 
      Sciences Rasht, Iran.
FAU - Khalili-Samani, Saman
AU  - Khalili-Samani S
AD  - Department of Microbiology, School of Biology, University College of Sciences, 
      University of Tehran Tehran, Iran.
LA  - eng
PT  - Journal Article
DEP - 20160525
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC4879133
OTO - NOTNLM
OT  - C. albicans
OT  - H. pylori
OT  - coinfection
OT  - gastric diseases
EDAT- 2016/06/03 06:00
MHDA- 2016/06/03 06:01
PMCR- 2016/05/25
CRDT- 2016/06/03 06:00
PHST- 2016/02/20 00:00 [received]
PHST- 2016/05/11 00:00 [accepted]
PHST- 2016/06/03 06:00 [entrez]
PHST- 2016/06/03 06:00 [pubmed]
PHST- 2016/06/03 06:01 [medline]
PHST- 2016/05/25 00:00 [pmc-release]
AID - 10.3389/fmicb.2016.00801 [doi]
PST - epublish
SO  - Front Microbiol. 2016 May 25;7:801. doi: 10.3389/fmicb.2016.00801. eCollection 
      2016.