PMID- 2725500
OWN - NLM
STAT- MEDLINE
DCOM- 19890623
LR  - 20240109
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 9
IP  - 3
DP  - 1989 Mar
TI  - Cytoplasmic heat shock granules are formed from precursor particles and are 
      associated with a specific set of mRNAs.
PG  - 1298-308
AB  - In heat-shocked tomato cell cultures, cytoplasmic heat shock granules (HSGs) are 
      tightly associated with a specific subset of mRNAs coding mainly for the 
      untranslated control proteins. This messenger ribonucleoprotein complex was 
      banded in a CsCl gradient after fixation with formaldehyde (approximately 1.30 
      g/cm3). It contains all the heat shock proteins and most of the RNA applied to 
      the gradient. During heat shock, a reversible aggregation of HSGs from 15S 
      precursor particles can be shown. These pre-HSGs are not identical to the 19S 
      plant prosomes. Ultrastructural analysis supports the ribonucleoprotein nature of 
      HSGs and their composition of approximately 10-nm precursor particles. A model 
      summarizes our results. It gives a reasonable explanation for the striking 
      conservation of untranslated mRNAs during heat shock and may apply also to animal 
      cells.
FAU - Nover, L
AU  - Nover L
AD  - Institute of Plant Biochemistry, Academy of Sciences of the GDR, Halle.
FAU - Scharf, K D
AU  - Scharf KD
FAU - Neumann, D
AU  - Neumann D
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Plant Proteins)
RN  - 0 (Protein Precursors)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Cytoplasmic Granules/metabolism/ultrastructure
MH  - Heat-Shock Proteins/*biosynthesis/genetics
MH  - Microscopy, Electron
MH  - Models, Biological
MH  - Plant Proteins/biosynthesis/genetics
MH  - Plants/genetics/metabolism/ultrastructure
MH  - Protein Biosynthesis
MH  - Protein Precursors/genetics/metabolism
MH  - RNA, Messenger/genetics/*metabolism
PMC - PMC362722
EDAT- 1989/03/01 00:00
MHDA- 1989/03/01 00:01
PMCR- 1989/03/01
CRDT- 1989/03/01 00:00
PHST- 1989/03/01 00:00 [pubmed]
PHST- 1989/03/01 00:01 [medline]
PHST- 1989/03/01 00:00 [entrez]
PHST- 1989/03/01 00:00 [pmc-release]
AID - 10.1128/mcb.9.3.1298-1308.1989 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1989 Mar;9(3):1298-308. doi: 10.1128/mcb.9.3.1298-1308.1989.