PMID- 27255513
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160712
LR  - 20240325
IS  - 2041-2851 (Print)
IS  - 2041-2851 (Electronic)
VI  - 8
DP  - 2016
TI  - Genome evolution in alpine oat-like grasses through homoploid hybridization and 
      polyploidy.
LID - plw039 [pii]
LID - 10.1093/aobpla/plw039 [doi]
AB  - Hybridization and polyploidization can radically impact genome organization from 
      sequence level to chromosome structure. As a result, often in response to 
      environmental change and species isolation, the development of novel traits can 
      arise and will tend to result in the formation of homoploid or polyploid hybrid 
      species. In this study we focus on evidence of hybridization and polyploidization 
      by ascertaining the species parentage of the endemic alpine Helictotrichon 
      parlatorei group. This group comprises five taxa; the diploids H. parlatorei, 
      Helictotrichon setaceum subsp. setaceum and subsp. petzense, their putative 
      hybrid Helictotrichon xkrischae and the hexaploid Helictotrichon sempervirens. 
      For molecular analyses, cloned nuclear Topoisomerase VI genes of H. sempervirens 
      and H. xkrischae were sequenced and compared with sequences of the diploids to 
      estimate the evolutionary history in this group. In addition, detailed chromosome 
      studies were carried out including fluorescence in situ hybridization (FISH) with 
      5S and 45S ribosomal and satellite DNA probes, and fluorochrome staining with 
      chromomycin and DAPI. Two distinct types of Topoisomerase VI sequences were 
      identified. One of them (SET) occurs in both subspecies of H. setaceum, the other 
      (PAR) in H. parlatorei. Both types were found in H. xkrischae and H. sempervirens 
      Karyotypes of H. parlatorei and H. setaceum could be distinguished by chromosomes 
      with a clearly differentiated banding pattern of ribosomal DNAs. Both patterns 
      occurred in the hybrid H. xkrischae Hexaploid H. sempervirens shares karyotype 
      features with diploid H. parlatorei, but lacks the expected chromosome 
      characteristics of H. setaceum, possibly an example of beginning diploidization 
      after polyploidization. The geographic origin of the putative parental species 
      and their hybrids and the possible biogeographical spread through the Alps are 
      discussed.
CI  - Published by Oxford University Press on behalf of the Annals of Botany Company.
FAU - Winterfeld, Grit
AU  - Winterfeld G
AD  - Institute of Biology, Martin Luther University Halle-Wittenberg, Neuwerk 21, 
      06099 Halle, Germany gwinterfeld@gmx.net.
FAU - Wolk, Alexandra
AU  - Wolk A
AD  - Institute of Biology, Martin Luther University Halle-Wittenberg, Neuwerk 21, 
      06099 Halle, Germany.
FAU - Roser, Martin
AU  - Roser M
AD  - Institute of Biology, Martin Luther University Halle-Wittenberg, Neuwerk 21, 
      06099 Halle, Germany.
LA  - eng
PT  - Journal Article
DEP - 20160711
PL  - England
TA  - AoB Plants
JT  - AoB PLANTS
JID - 101539425
PMC - PMC4940509
OTO - NOTNLM
OT  - Alps
OT  - Helictotrichon
OT  - Poaceae
OT  - Topoisomerase VI
OT  - hybridization
OT  - karyotype
OT  - polyploidy
EDAT- 2016/06/04 06:00
MHDA- 2016/06/04 06:01
PMCR- 2016/01/01
CRDT- 2016/06/04 06:00
PHST- 2015/07/31 00:00 [received]
PHST- 2016/05/07 00:00 [accepted]
PHST- 2016/06/04 06:00 [entrez]
PHST- 2016/06/04 06:00 [pubmed]
PHST- 2016/06/04 06:01 [medline]
PHST- 2016/01/01 00:00 [pmc-release]
AID - plw039 [pii]
AID - 10.1093/aobpla/plw039 [doi]
PST - epublish
SO  - AoB Plants. 2016 Jul 11;8:plw039. doi: 10.1093/aobpla/plw039. Print 2016.