PMID- 27255587
OWN - NLM
STAT- MEDLINE
DCOM- 20170117
LR  - 20181113
IS  - 1472-6882 (Electronic)
IS  - 1472-6882 (Linking)
VI  - 16
DP  - 2016 Jun 3
TI  - Phytochemical profiles and inhibitory effects of Tiger Milk mushroom (Lignosus 
      rhinocerus) extract on ovalbumin-induced airway inflammation in a rodent model of 
      asthma.
PG  - 167
LID - 10.1186/s12906-016-1141-x [doi]
LID - 167
AB  - BACKGROUND: Lignosus rhinocerus (L. rhinocerus), which is known locally as Tiger 
      Milk mushroom, is traditionally used in the treatment of asthma by indigenous 
      communities in Malaysia. However, to date, its efficacy on asthma has not been 
      confirmed by scientific studies and there is also sparse information available on 
      its active constituents. In this study, the volatile constituent of L. rhinocerus 
      hot water extract was investigated using gas chromatography mass spectrometry 
      (GC-MS). The potential effects of L. rhinocerus extract for anti-asthmatic 
      activity was further investigated on ovalbumin (OVA)-sensitized asthmatic Sprague 
      Dawley rats. METHODS: Sequential extraction using five solvents (petroleum ether, 
      diethyl ether, hexane, ethyl acetate and methanol) was conducted prior to GC-MS 
      analysis. Male Sprague Dawley rats were divided into the following four groups of 
      five animals each: 1) normal rats, 2) sensitization plus OVA-challenged rats 3) 
      sensitization plus OVA-challenged with L. rhinocerus treatment and 4) 
      sensitization plus OVA-challenged with dexamethasone treatment. The levels of 
      immunoglobulin E (IgE) in the serum and T-helper 2 cytokines, including 
      interleukin (IL)-4, IL-5 and IL-13, in bronchoalveolar lavage fluid (BALF), as 
      well as eosinophil infiltration in the lungs, were investigated. RESULTS: GC-MS 
      analysis revealed the presence of five main groups (alkane, fatty acids, benzene, 
      phenol and dicarboxylic acid) with a total of 18 constituents. Linoleic acid 
      (21.35 %), octadecane (11.82 %) and 2,3-dihydroxypropyl elaidate (10.47 %) were 
      present in high amounts. The extract significantly ameliorated the increase in 
      total IgE in serum and IL-4, IL-5 and IL-13 levels in BALF and also effectively 
      suppressed eosinophils numbers in BALF while attenuating eosinophil infiltrations 
      in the lungs. CONCLUSION: L. rhinocerus hot water extract has the potential to be 
      used as an alternative for the treatment of acute asthma.
FAU - Johnathan, M
AU  - Johnathan M
AD  - School of Dental Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, 
      Kelantan, Malaysia.
FAU - Gan, S H
AU  - Gan SH
AD  - Human Genome Centre, School of Medical Sciences, Universiti Sains Malaysia, 
      16150, Kubang Kerian, Kelantan, Malaysia.
FAU - Ezumi, M F Wan
AU  - Ezumi MF
AD  - School of Health Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, 
      Kelantan, Malaysia.
FAU - Faezahtul, A H
AU  - Faezahtul AH
AD  - Department of Pathology, School of Medical Sciences, Universiti Sains Malaysia, 
      16150, Kubang Kerian, Kelantan, Malaysia.
FAU - Nurul, A A
AU  - Nurul AA
AD  - School of Health Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, 
      Kelantan, Malaysia. nurulasma@usm.my.
LA  - eng
PT  - Journal Article
DEP - 20160603
PL  - England
TA  - BMC Complement Altern Med
JT  - BMC complementary and alternative medicine
JID - 101088661
RN  - 0 (Anti-Asthmatic Agents)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Animals
MH  - Anti-Asthmatic Agents/pharmacology
MH  - Asthma/chemically induced/*drug therapy
MH  - Disease Models, Animal
MH  - Gas Chromatography-Mass Spectrometry
MH  - Inflammation
MH  - Male
MH  - Ovalbumin
MH  - Polyporaceae/*chemistry
MH  - Rats
MH  - Rats, Sprague-Dawley
PMC - PMC4891846
OTO - NOTNLM
OT  - Airway inflammation
OT  - Asthma
OT  - GC-MS
OT  - Lignosus rhinocerus
OT  - Linoleic acid
OT  - Tiger milk mushroom
EDAT- 2016/06/04 06:00
MHDA- 2017/01/18 06:00
PMCR- 2016/06/03
CRDT- 2016/06/04 06:00
PHST- 2015/10/31 00:00 [received]
PHST- 2016/05/25 00:00 [accepted]
PHST- 2016/06/04 06:00 [entrez]
PHST- 2016/06/04 06:00 [pubmed]
PHST- 2017/01/18 06:00 [medline]
PHST- 2016/06/03 00:00 [pmc-release]
AID - 10.1186/s12906-016-1141-x [pii]
AID - 1141 [pii]
AID - 10.1186/s12906-016-1141-x [doi]
PST - epublish
SO  - BMC Complement Altern Med. 2016 Jun 3;16:167. doi: 10.1186/s12906-016-1141-x.