PMID- 27256676
OWN - NLM
STAT- MEDLINE
DCOM- 20170609
LR  - 20220316
IS  - 1862-1783 (Electronic)
IS  - 1673-1581 (Print)
IS  - 1673-1581 (Linking)
VI  - 17
IP  - 6
DP  - 2016 Jun
TI  - Inhibitory effect of Gardenblue blueberry (Vaccinium ashei Reade) anthocyanin 
      extracts on lipopolysaccharide-stimulated inflammatory response in RAW 264.7 
      cells.
PG  - 425-36
LID - 10.1631/jzus.B1500213 [doi]
AB  - Blueberries are a rich source of anthocyanins, which are associated with health 
      benefits contributing to a reduced risk for many diseases. The present study 
      identified the functional Gardenblue blueberry (Vaccinium ashei Reade) 
      anthocyanin extracts (GBBAEs) and evaluated their capacity and underlying 
      mechanisms in protecting murine RAW 264.7 cells from lipopolysaccharide 
      (LPS)-stimulated inflammation in vitro. Enzyme-linked immunosorbent assay (ELISA) 
      kit results showed that GBBAEs significantly inhibited the production of nitric 
      oxide (NO), prostaglandin E2 (PGE2), interleukin-6 (IL-6), IL-1beta, and 
      interferon-gamma (INF-gamma). Real-time polymerase chain reaction (PCR) analysis 
      indicated that the mRNA expression levels of IL-6, IL-1beta, tumor necrosis factor-alpha 
      (TNF-alpha), monocyte chemoattractant protein-1 (MCP-1), and cyclooxygenase 2 (COX-2) 
      were suppressed in LPS-stimulated RAW 264.7 cells. Additionally, Western blot 
      analysis was used to evaluate the relative protein expression levels of COX-2 and 
      nuclear factor-kappaB p65 (NF-kappaBp65). All these results suggested the potential use 
      of GBBAEs as a functional food for the treatment of inflammatory diseases.
FAU - Xu, Wei
AU  - Xu W
AD  - College of Food Science and Technology, Huazhong Agricultural University, Wuhan 
      430070, China.
AD  - Research Center for Food Safety and Nutrition, Key Lab of Urban Agriculture 
      (South), Bor S. Luh Food Safety Research Center, School of Agriculture & Biology, 
      Shanghai Jiao Tong University, Shanghai 200240, China.
FAU - Zhou, Qing
AU  - Zhou Q
AD  - Department of Pharmacy, Wuhan City Central Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan 430014, China.
FAU - Yao, Yong
AU  - Yao Y
AD  - College of Food Science and Technology, Huazhong Agricultural University, Wuhan 
      430070, China.
FAU - Li, Xing
AU  - Li X
AD  - College of Food Science and Technology, Huazhong Agricultural University, Wuhan 
      430070, China.
FAU - Zhang, Jiu-Liang
AU  - Zhang JL
AD  - College of Food Science and Technology, Huazhong Agricultural University, Wuhan 
      430070, China.
AD  - Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural 
      University), Ministry of Education, Wuhan 430070, China.
FAU - Su, Guan-Hua
AU  - Su GH
AD  - Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430022, China.
FAU - Deng, Ai-Ping
AU  - Deng AP
AD  - Department of Pharmacy, Wuhan City Central Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan 430014, China.
LA  - eng
PT  - Journal Article
PL  - China
TA  - J Zhejiang Univ Sci B
JT  - Journal of Zhejiang University. Science. B
JID - 101236535
RN  - 0 (Anthocyanins)
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Plant Extracts)
RN  - 0 (Rela protein, mouse)
RN  - 0 (Transcription Factor RelA)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
SB  - IM
MH  - Animals
MH  - Anthocyanins/*pharmacology
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - *Blueberry Plants
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Cyclooxygenase 2/genetics
MH  - Cytokines/genetics
MH  - Lipopolysaccharides/pharmacology
MH  - Mice
MH  - Nitric Oxide/biosynthesis
MH  - Plant Extracts/*pharmacology
MH  - Transcription Factor RelA/genetics
PMC - PMC4913791
OTO - NOTNLM
OT  - Anti-inflammatory
OT  - Cyclooxygenase 2 (COX-2)
OT  - Gardenblue blueberry (Vaccinium ashei Reade) anthocyanin extracts (GBBAEs)
OT  - Nuclear factor-kappaB p65 (NF-kappaBp65)
OT  - RAW 264.7
COIS- Compliance with ethics guidelines: Wei XU, Qing ZHOU, Yong YAO, Xing LI, 
      Jiu-liang ZHANG, Guan-hua SU, and Ai-ping DENG declare that they have no conflict 
      of interest. This article does not contain any studies with human or animal 
      subjects performed by any of the authors.
EDAT- 2016/06/04 06:00
MHDA- 2017/06/10 06:00
PMCR- 2016/06/01
CRDT- 2016/06/04 06:00
PHST- 2016/06/04 06:00 [entrez]
PHST- 2016/06/04 06:00 [pubmed]
PHST- 2017/06/10 06:00 [medline]
PHST- 2016/06/01 00:00 [pmc-release]
AID - 10.1631/jzus.B1500213 [doi]
PST - ppublish
SO  - J Zhejiang Univ Sci B. 2016 Jun;17(6):425-36. doi: 10.1631/jzus.B1500213.