PMID- 27256897
OWN - NLM
STAT- MEDLINE
DCOM- 20160922
LR  - 20220204
IS  - 1751-8741 (Print)
IS  - 1751-875X (Electronic)
IS  - 1751-8741 (Linking)
VI  - 10
IP  - 3
DP  - 2016 Jun
TI  - Optimisation of nitrate reductase enzyme activity to synthesise silver 
      nanoparticles.
PG  - 158-61
LID - 10.1049/iet-nbt.2015.0062 [doi]
AB  - Today, the synthesis of silver nanoparticles (Ag NPs) is very common since it has 
      many applications in different areas. The synthesis of these nanoparticles is 
      done by means of physical, chemical, or biological methods. However, due to its 
      inexpensive and environmentally friendly features, the biological method is more 
      preferable. In the present study, using nitrate reductase enzyme available in the 
      Escherichia coli (E. coli) bacterium, the biosynthesis of Ag NPs was 
      investigated. In addition, the activity of the nitrate reductase enzyme was 
      optimised by changing its cultural conditions, and the effects of silver nitrate 
      (AgNO(3)) concentration and enzyme amount on nanoparticles synthesis were 
      studied. Finally, the produced nanoparticles were studied using ultraviolet 
      -visible (UV-Vis) spectrophotometer, dynamic light scattering technique, and 
      transmission electron microscopy. UV-Visible spectrophotometric study showed the 
      characteristic peak for Ag NPs at wavelength 405-420 nm for 1 mM metal precursor 
      solution (AgNO(3)) with 1, 5, 10, and 20 cc supernatant and 435 nm for 0.01M 
      AgNO(3) with 20 cc supernatant. In this study, it was found that there is a 
      direct relationship between the AgNO(3) concentration and the size of produced Ag 
      NPs.
FAU - Khodashenas, Bahareh
AU  - Khodashenas B
AD  - Department of Chemical Engineering, Shahrood Branch, Islamic Azad University, 
      Shahrood, Iran. bahar.khodashenas67@gmail.com.
FAU - Ghorbani, Hamid Reza
AU  - Ghorbani HR
AD  - Department of Chemical Engineering, Central Tehran Branch, Islamic Azad 
      University, Tehran, Iran.
LA  - eng
PT  - Journal Article
PL  - England
TA  - IET Nanobiotechnol
JT  - IET nanobiotechnology
JID - 101303205
RN  - 0 (Escherichia coli Proteins)
RN  - 3M4G523W1G (Silver)
RN  - EC 1.7.- (Nitrate Reductases)
SB  - IM
MH  - Bioreactors/microbiology
MH  - Dynamic Light Scattering
MH  - Escherichia coli/enzymology/metabolism
MH  - Escherichia coli Proteins/chemistry/*metabolism
MH  - Metal Nanoparticles/*chemistry
MH  - Microscopy, Electron, Transmission
MH  - Nitrate Reductases/chemistry/*metabolism
MH  - Particle Size
MH  - Silver/chemistry/*metabolism
PMC - PMC8676541
EDAT- 2016/06/04 06:00
MHDA- 2016/09/23 06:00
PMCR- 2016/06/01
CRDT- 2016/06/04 06:00
PHST- 2016/06/04 06:00 [entrez]
PHST- 2016/06/04 06:00 [pubmed]
PHST- 2016/09/23 06:00 [medline]
PHST- 2016/06/01 00:00 [pmc-release]
AID - NBT2BF00142 [pii]
AID - 10.1049/iet-nbt.2015.0062 [doi]
PST - ppublish
SO  - IET Nanobiotechnol. 2016 Jun;10(3):158-61. doi: 10.1049/iet-nbt.2015.0062.