PMID- 27257817
OWN - NLM
STAT- MEDLINE
DCOM- 20170818
LR  - 20190213
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 11
IP  - 6
DP  - 2016
TI  - Transcriptional Activity of rRNA Genes in Barley Cells after Mutagenic Treatment.
PG  - e0156865
LID - 10.1371/journal.pone.0156865 [doi]
LID - e0156865
AB  - In the present study, the combination of the micronucleus test with analysis of 
      the activity of the rRNA genes in mutagen-treated Hordeum vulgare (barley) by 
      maleic hydrazide (MH) cells was performed. Simultaneously fluorescence in situ 
      hybridization (FISH) with 25S rDNA as probes and an analysis of the 
      transcriptional activity of 35S rRNA genes with silver staining were performed. 
      The results showed that transcriptional activity is always maintained in the 
      micronuclei although they are eliminated during the next cell cycle. The analysis 
      of the transcriptional activity was extended to barley nuclei. MH influenced the 
      fusion of the nucleoli in barley nuclei. The silver staining enabled detection of 
      the nuclear bodies which arose after MH treatment. The results confirmed the 
      usefulness of cytogenetic techniques in the characterization of micronuclei. 
      Similar analyses can be now extended to other abiotic stresses to study the 
      response of plant cells to the environment.
FAU - Kwasniewska, Jolanta
AU  - Kwasniewska J
AD  - Department of Plant Anatomy and Cytology, Faculty of Biology and Environmental 
      Protection, University of Silesia in Katowice, Katowice, Poland.
FAU - Jaskowiak, Joanna
AU  - Jaskowiak J
AD  - Department of Plant Anatomy and Cytology, Faculty of Biology and Environmental 
      Protection, University of Silesia in Katowice, Katowice, Poland.
LA  - eng
PT  - Journal Article
DEP - 20160603
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (DNA, Plant)
RN  - 0 (Mutagens)
RN  - 0 (RNA, Ribosomal)
RN  - TQ7L3FCV66 (Maleic Hydrazide)
SB  - IM
MH  - Chromosomes, Plant/genetics
MH  - DNA, Plant/genetics
MH  - Hordeum/drug effects/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Maleic Hydrazide/toxicity
MH  - Micronucleus Tests
MH  - Mutagenesis/drug effects/genetics
MH  - Mutagens/toxicity
MH  - RNA, Ribosomal/*genetics
MH  - Transcriptional Activation/drug effects/genetics
PMC - PMC4892555
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2016/06/04 06:00
MHDA- 2017/08/19 06:00
PMCR- 2016/06/03
CRDT- 2016/06/04 06:00
PHST- 2016/02/25 00:00 [received]
PHST- 2016/05/01 00:00 [accepted]
PHST- 2016/06/04 06:00 [entrez]
PHST- 2016/06/04 06:00 [pubmed]
PHST- 2017/08/19 06:00 [medline]
PHST- 2016/06/03 00:00 [pmc-release]
AID - PONE-D-16-08119 [pii]
AID - 10.1371/journal.pone.0156865 [doi]
PST - epublish
SO  - PLoS One. 2016 Jun 3;11(6):e0156865. doi: 10.1371/journal.pone.0156865. 
      eCollection 2016.