PMID- 27258938
OWN - NLM
STAT- MEDLINE
DCOM- 20170626
LR  - 20180501
IS  - 1945-7197 (Electronic)
IS  - 0021-972X (Linking)
VI  - 101
IP  - 8
DP  - 2016 Aug
TI  - Insulin Plays a Permissive Role for the Vasoactive Effect of GIP Regulating 
      Adipose Tissue Metabolism in Humans.
PG  - 3155-62
LID - 10.1210/jc.2016-1933 [doi]
AB  - CONTEXT AND OBJECTIVE: Glucose-dependent insulinotropic polypeptide (GIP) in 
      combination with hyperinsulinemia increases blood flow and triglyceride (TAG) 
      clearance in subcutaneous (sc) abdominal adipose tissue in lean humans. The 
      present experiments were performed to further investigate the role of insulin for 
      the vasoactive effect of GIP in adipose tissue metabolism and whether the 
      vasodilatory effect of GIP is dependent on C-peptide. METHODS: Six lean healthy 
      subjects were studied. The sc abdominal adipose tissue metabolism was assessed by 
      Fick's principle during GIP infusion (1.5 pmol/kg/min) in combination with 1) 
      euglycemic-high insulinemic clamp (Eugluc-Hiinsu), raising plasma insulin 
      concentrations to postprandial levels, 2) hyperglycemic-euinsulinemic clamp 
      (Hygluc-Euinsu), and 3) hyperglycemic-hyperinsulinemic clamp, raising plasma 
      insulin concentrations to supraphysiological levels. During the hyperglycemic 
      clamps, endogenous insulin and C-peptide secretion were inhibited by infusion of 
      the somatostatin analogue octreotide. RESULTS: During GIP infusion, 
      Eugluc-Hiinsu, and hyperglycemic-hyperinsulinemic clamps, sc abdominal adipose 
      tissue blood flow (ATBF) was similar and increased from 2.1 +/- 0.2 and 2.2 +/- 0.4 
      ml min(-1) (100 g tissue)(-1) to 7.1 +/- 0.6 and 7.6 +/- 0.1 ml min(-1) (100 g 
      tissue)(-1), respectively (P < .01). ATBF remained virtually constant (2.7 +/- 0.4 
      ml min(-1) [100 g tissue](-1)) during Hygluc-Euinsu and GIP infusion. In 
      addition, adipose tissue TAG clearance increased significantly (P = .03), whereas 
      free fatty acid output (P = .01), glycerol output (P = .02) and free fatty 
      acid/glycerol release ratio (P = .04) decreased during the Eugluc-Hiinsu clamp 
      compared to Hygluc-Euinsu clamp with GIP. CONCLUSION: In healthy lean humans, 
      insulin is permissive for GIP to induce an increase in blood flow and TAG 
      clearance in sc abdominal adipose tissue. This effect is independent of 
      C-peptide.
FAU - Asmar, Meena
AU  - Asmar M
AD  - Department of Clinical Physiology/Nuclear Medicine (M.A., L.S., A.A., J.B.), 
      Bispebjerg University Hospital, Copenhagen, Denmark; The Novo Nordisk Foundation 
      Center for Basic Metabolic Research (J.J.H.), University of Copenhagen, 
      Copenhagen, Denmark; Department of Biomedical Sciences (J.J.H., J.B.), University 
      of Copenhagen, Copenhagen, Denmark; Xlab, Center for Healthy Ageing (F.D.), 
      University of Copenhagen, Denmark; Department of Geriatrics (F.D.), Bispebjerg 
      University Hospital, Copenhagen, Denmark.
FAU - Simonsen, Lene
AU  - Simonsen L
AD  - Department of Clinical Physiology/Nuclear Medicine (M.A., L.S., A.A., J.B.), 
      Bispebjerg University Hospital, Copenhagen, Denmark; The Novo Nordisk Foundation 
      Center for Basic Metabolic Research (J.J.H.), University of Copenhagen, 
      Copenhagen, Denmark; Department of Biomedical Sciences (J.J.H., J.B.), University 
      of Copenhagen, Copenhagen, Denmark; Xlab, Center for Healthy Ageing (F.D.), 
      University of Copenhagen, Denmark; Department of Geriatrics (F.D.), Bispebjerg 
      University Hospital, Copenhagen, Denmark.
FAU - Asmar, Ali
AU  - Asmar A
AD  - Department of Clinical Physiology/Nuclear Medicine (M.A., L.S., A.A., J.B.), 
      Bispebjerg University Hospital, Copenhagen, Denmark; The Novo Nordisk Foundation 
      Center for Basic Metabolic Research (J.J.H.), University of Copenhagen, 
      Copenhagen, Denmark; Department of Biomedical Sciences (J.J.H., J.B.), University 
      of Copenhagen, Copenhagen, Denmark; Xlab, Center for Healthy Ageing (F.D.), 
      University of Copenhagen, Denmark; Department of Geriatrics (F.D.), Bispebjerg 
      University Hospital, Copenhagen, Denmark.
FAU - Holst, Jens Juul
AU  - Holst JJ
AD  - Department of Clinical Physiology/Nuclear Medicine (M.A., L.S., A.A., J.B.), 
      Bispebjerg University Hospital, Copenhagen, Denmark; The Novo Nordisk Foundation 
      Center for Basic Metabolic Research (J.J.H.), University of Copenhagen, 
      Copenhagen, Denmark; Department of Biomedical Sciences (J.J.H., J.B.), University 
      of Copenhagen, Copenhagen, Denmark; Xlab, Center for Healthy Ageing (F.D.), 
      University of Copenhagen, Denmark; Department of Geriatrics (F.D.), Bispebjerg 
      University Hospital, Copenhagen, Denmark.
FAU - Dela, Flemming
AU  - Dela F
AD  - Department of Clinical Physiology/Nuclear Medicine (M.A., L.S., A.A., J.B.), 
      Bispebjerg University Hospital, Copenhagen, Denmark; The Novo Nordisk Foundation 
      Center for Basic Metabolic Research (J.J.H.), University of Copenhagen, 
      Copenhagen, Denmark; Department of Biomedical Sciences (J.J.H., J.B.), University 
      of Copenhagen, Copenhagen, Denmark; Xlab, Center for Healthy Ageing (F.D.), 
      University of Copenhagen, Denmark; Department of Geriatrics (F.D.), Bispebjerg 
      University Hospital, Copenhagen, Denmark.
FAU - Bulow, Jens
AU  - Bulow J
AD  - Department of Clinical Physiology/Nuclear Medicine (M.A., L.S., A.A., J.B.), 
      Bispebjerg University Hospital, Copenhagen, Denmark; The Novo Nordisk Foundation 
      Center for Basic Metabolic Research (J.J.H.), University of Copenhagen, 
      Copenhagen, Denmark; Department of Biomedical Sciences (J.J.H., J.B.), University 
      of Copenhagen, Copenhagen, Denmark; Xlab, Center for Healthy Ageing (F.D.), 
      University of Copenhagen, Denmark; Department of Geriatrics (F.D.), Bispebjerg 
      University Hospital, Copenhagen, Denmark.
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial
DEP - 20160603
PL  - United States
TA  - J Clin Endocrinol Metab
JT  - The Journal of clinical endocrinology and metabolism
JID - 0375362
RN  - 0 (Insulin)
RN  - 0 (Vasoconstrictor Agents)
RN  - 59392-49-3 (Gastric Inhibitory Polypeptide)
SB  - IM
MH  - Adipose Tissue/blood supply/*drug effects/*metabolism
MH  - Adult
MH  - Cross-Over Studies
MH  - Drug Synergism
MH  - Gastric Inhibitory Polypeptide/*pharmacology
MH  - Glucose Clamp Technique
MH  - Humans
MH  - Insulin/*pharmacology
MH  - Male
MH  - Regional Blood Flow/drug effects
MH  - Subcutaneous Fat, Abdominal/blood supply/drug effects/metabolism
MH  - Vasoconstriction/*drug effects
MH  - Vasoconstrictor Agents/pharmacology
MH  - Young Adult
EDAT- 2016/06/04 06:00
MHDA- 2017/06/27 06:00
CRDT- 2016/06/04 06:00
PHST- 2016/06/04 06:00 [entrez]
PHST- 2016/06/04 06:00 [pubmed]
PHST- 2017/06/27 06:00 [medline]
AID - 10.1210/jc.2016-1933 [doi]
PST - ppublish
SO  - J Clin Endocrinol Metab. 2016 Aug;101(8):3155-62. doi: 10.1210/jc.2016-1933. Epub 
      2016 Jun 3.