PMID- 27261611
OWN - NLM
STAT- MEDLINE
DCOM- 20170131
LR  - 20180412
IS  - 1950-6007 (Electronic)
IS  - 0753-3322 (Linking)
VI  - 81
DP  - 2016 Jul
TI  - Protection of rats spinal cord ischemia-reperfusion injury by inhibition of 
      MiR-497 on inflammation and apoptosis: Possible role in pediatrics.
PG  - 337-344
LID - S0753-3322(16)30077-4 [pii]
LID - 10.1016/j.biopha.2016.04.028 [doi]
AB  - MicroRNAs are extensively included in the pathogenesis and progression of many 
      diseases by inhibiting target gene expression. Recently, studies have 
      demonstrated that microRNA-497 (miR-497) may be implicated in human breast cancer 
      that miR-497 predicts the prognosis of breast cancer patients from the 
      posttranscriptional level. However, the specific function of miR-497 in spinal 
      cord ischemia-reperfusion (IR) injury is far from clear nowadays. The present 
      study was designed to determine the role of miR-497 in spinal cord IR injury and 
      investigate the underlying spinal cord protective mechanism. The rat spinal cord 
      IR injury model was performed by occluding the left anterior descending coronary 
      artery for 30 min, which is then followed by 12h reperfusion. As predicted, 
      miR-497 over-expression markedly decreased the expression of IL-1 receptor 
      associated kinase (IRAK1) and Cyclic AMP response element binding protein (CREB). 
      Moreover, Toll-like receptor 4 (TLR4), nuclear factor-kappa B (NF-kappaB) and 
      Caspase-3, as miR-497 potential targets were significantly suppressed after 
      miR-497 transfection, then preventing inflammatory cytokines and factors 
      regulating apoptosis. We also found that tumor necrosis factor-a (TNF-alpha) and 
      interleukin-1beta (IL-1beta) activity, pro-apoptotic related genes, such as 
      extracellular regulated protein kinases (ERK), Bcl-2 Associated X Protein (Bax), 
      Bcl-2, Bcl-xL levels were all decreased associated with the down-regulation of 
      IRAK1 and CREB. In conclusion, our data demonstrate that miR-497 could inhibit 
      inflammation and apoptosis of spinal cord IR through its targets, IRAK1 of TLR4 
      and CREB signaling pathway. Thus, miR-497 may constitute a new therapeutic target 
      for the prevention of spinal cord IR injury.
CI  - Copyright (c) 2016 Elsevier Masson SAS. All rights reserved.
FAU - Xu, Meng
AU  - Xu M
AD  - Department of Pediatric Surgery, Yishui Central Hospital, Linyi, Shandong 276400, 
      China.
FAU - Wang, Hai-Feng
AU  - Wang HF
AD  - Department of Pediatric Surgery, Yishui Central Hospital, Linyi, Shandong 276400, 
      China.
FAU - Zhang, Ying-Ying
AU  - Zhang YY
AD  - Department of Hand and Foot Surgery, Yishui Central Hospital, Linyi, Shandong 
      276400, China.
FAU - Zhuang, Hui-Wen
AU  - Zhuang HW
AD  - Department of Pediatric Surgery, Yishui Central Hospital, Linyi, Shandong 276400, 
      China.
LA  - eng
PT  - Journal Article
PT  - Retracted Publication
DEP - 20160425
PL  - France
TA  - Biomed Pharmacother
JT  - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
JID - 8213295
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
RN  - 0 (MIRN497 microRNA, rat)
RN  - 0 (MicroRNAs)
RN  - 0 (NF-kappa B)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Toll-Like Receptor 4)
RN  - EC 2.7.11.1 (IRAK1 protein, rat)
RN  - EC 2.7.11.1 (Interleukin-1 Receptor-Associated Kinases)
SB  - IM
RIN - Biomed Pharmacother. 2018 Feb;98 :927. PMID: 29421863
MH  - Animals
MH  - *Apoptosis
MH  - Base Sequence
MH  - Cell Line, Tumor
MH  - Cyclic AMP Response Element-Binding Protein/metabolism
MH  - Cytokines/metabolism
MH  - Humans
MH  - Inflammation/*pathology
MH  - Inflammation Mediators/metabolism
MH  - Injections, Spinal
MH  - Interleukin-1 Receptor-Associated Kinases/metabolism
MH  - Male
MH  - MicroRNAs/genetics/metabolism
MH  - NF-kappa B/metabolism
MH  - Neuroprotective Agents/*metabolism
MH  - *Pediatrics
MH  - Rats, Sprague-Dawley
MH  - Reperfusion Injury/*genetics/*pathology
MH  - Spinal Cord/*blood supply/*pathology
MH  - Toll-Like Receptor 4/metabolism
OTO - NOTNLM
OT  - CREB
OT  - IRAK1
OT  - Spinal cord ischemia-reperfusion
OT  - TLR4
OT  - miR-497
EDAT- 2016/06/05 06:00
MHDA- 2017/02/01 06:00
CRDT- 2016/06/05 06:00
PHST- 2016/01/20 00:00 [received]
PHST- 2016/04/08 00:00 [revised]
PHST- 2016/04/11 00:00 [accepted]
PHST- 2016/06/05 06:00 [entrez]
PHST- 2016/06/05 06:00 [pubmed]
PHST- 2017/02/01 06:00 [medline]
AID - S0753-3322(16)30077-4 [pii]
AID - 10.1016/j.biopha.2016.04.028 [doi]
PST - ppublish
SO  - Biomed Pharmacother. 2016 Jul;81:337-344. doi: 10.1016/j.biopha.2016.04.028. Epub 
      2016 Apr 25.