PMID- 27262294
OWN - NLM
STAT- MEDLINE
DCOM- 20170222
LR  - 20170222
IS  - 1573-6776 (Electronic)
IS  - 0141-5492 (Linking)
VI  - 38
IP  - 9
DP  - 2016 Sep
TI  - Enterococcus faecalis promotes osteoclast differentiation within an 
      osteoblast/osteoclast co-culture system.
PG  - 1443-8
LID - 10.1007/s10529-016-2142-z [doi]
AB  - OBJECTIVE: To investigate the effects of heat-killed Enterococcus faecalis ATCC 
      29212 and P25RC clinical strain (derived from an obturated root canal with apical 
      periodontitis) on osteoclast differentiation within an osteoblast/osteoclast 
      co-culture system. RESULTS: Heat-killed E. faecalis significantly increased the 
      proportion of multinucleated osteoclastic cells (MNCs) within the co-culture 
      system. The IL-6 level was significantly increased upon exposure to heat-killed 
      E. faecalis. Gene expression levels of NFATc1 and cathepsin K were significantly 
      up-regulated compared to the untreated control. EphrinB2 and EphB4 expressions at 
      both the mRNA and protein levels were also significantly upregulated compared to 
      the untreated control. CONCLUSIONS: Heat-killed E. faecalis can induce osteoclast 
      differentiation within the osteoblast/osteoclast co-culture system in vitro, 
      possibly through ephrinB2-EphB4 bidirectional signaling.
FAU - Deng, Zuhui
AU  - Deng Z
AD  - Endodontics, Comprehensive Dental Care, Faculty of Dentistry, The University of 
      Hong Kong, Hong Kong SAR, 999077, China.
AD  - Shenzhen ENT Institute, Shenzhen Longgang ENT Hospital, Shenzhen, 518172, 
      Guangdong, China.
FAU - Wang, Shuai
AU  - Wang S
AD  - Endodontics, Comprehensive Dental Care, Faculty of Dentistry, The University of 
      Hong Kong, Hong Kong SAR, 999077, China.
FAU - Heng, Boon Chin
AU  - Heng BC
AD  - Endodontics, Comprehensive Dental Care, Faculty of Dentistry, The University of 
      Hong Kong, Hong Kong SAR, 999077, China.
FAU - Yuan, Changyong
AU  - Yuan C
AD  - Endodontics, Comprehensive Dental Care, Faculty of Dentistry, The University of 
      Hong Kong, Hong Kong SAR, 999077, China.
FAU - Zhang, Chengfei
AU  - Zhang C
AD  - Endodontics, Comprehensive Dental Care, Faculty of Dentistry, The University of 
      Hong Kong, Hong Kong SAR, 999077, China. zhangcf@hku.hk.
LA  - eng
PT  - Journal Article
DEP - 20160604
PL  - Netherlands
TA  - Biotechnol Lett
JT  - Biotechnology letters
JID - 8008051
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cell Differentiation/genetics/physiology
MH  - Coculture Techniques
MH  - Enterococcus faecalis/*pathogenicity
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Mice
MH  - Osteoblasts/*microbiology
MH  - Osteoclasts/*microbiology
MH  - Real-Time Polymerase Chain Reaction
MH  - Signal Transduction/genetics/physiology
OTO - NOTNLM
OT  - Enterococcus faecalis
OT  - EphB4
OT  - EphrinB2
OT  - Multinucleated osteoclastic cells
OT  - Osteoblasts
OT  - Osteoclast differentiation
OT  - Osteoclasts
EDAT- 2016/06/06 06:00
MHDA- 2017/02/23 06:00
CRDT- 2016/06/06 06:00
PHST- 2016/04/27 00:00 [received]
PHST- 2016/05/24 00:00 [accepted]
PHST- 2016/06/06 06:00 [entrez]
PHST- 2016/06/06 06:00 [pubmed]
PHST- 2017/02/23 06:00 [medline]
AID - 10.1007/s10529-016-2142-z [pii]
AID - 10.1007/s10529-016-2142-z [doi]
PST - ppublish
SO  - Biotechnol Lett. 2016 Sep;38(9):1443-8. doi: 10.1007/s10529-016-2142-z. Epub 2016 
      Jun 4.