PMID- 27263830
OWN - NLM
STAT- MEDLINE
DCOM- 20180206
LR  - 20180416
IS  - 1872-8359 (Electronic)
IS  - 0167-7012 (Linking)
VI  - 128
DP  - 2016 Sep
TI  - Construction of probe of the plant growth-promoting bacteria Bacillus subtilis 
      useful for fluorescence in situ hybridization.
PG  - 125-129
LID - S0167-7012(16)30126-9 [pii]
LID - 10.1016/j.mimet.2016.05.029 [doi]
AB  - Strains of Bacillus subtilis are plant growth-promoting bacteria (PGPB) of many 
      crops and are used as inoculants. PGPB colonization is an important trait for 
      success of a PGPB on plants. A specific probe, based on the 16 s rRNA of Bacillus 
      subtilis, was designed and evaluated to distinguishing, by fluorescence in situ 
      hybridization (FISH), between this species and the closely related Bacillus 
      amyloliquefaciens. The selected target for the probe was between nucleotides 465 
      and 483 of the gene, where three different nucleotides can be identified. The 
      designed probe successfully hybridized with several strains of Bacillus subtilis, 
      but failed to hybridize not only with B. amyloliquefaciens, but also with other 
      strains such as Bacillus altitudinis, Bacillus cereus, Bacillus gibsonii, 
      Bacillus megaterium, Bacillus pumilus; and with the external phylogenetic strains 
      Azospirillum brasilense Cd, Micrococcus sp. and Paenibacillus sp. The results 
      showed the specificity of this molecular probe for B. subtilis.
FAU - Posada, Luisa F
AU  - Posada LF
AD  - Department of Process Engineering, Cra 49 #7 sur-50, Universidad EAFIT, Medellin, 
      Colombia.
FAU - Alvarez, Javier C
AU  - Alvarez JC
AD  - Departament of Biological Sciences, Cra 49 #7 sur-50, Universidad EAFIT, 
      Medellin, Colombia.
FAU - Hu, Chia-Hui
AU  - Hu CH
AD  - Department of Entomology and Plant Pathology, Auburn University, 301 Funchess 
      Hall, Auburn, AL 36849, USA.
FAU - de-Bashan, Luz E
AU  - de-Bashan LE
AD  - The Bashan Institute of Science, 1730 Post Oak Ct., AL 36830, USA; Environmental 
      Microbiology Group, Northwestern Center for Biological Research (CIBNOR), Av. IPN 
      195, La Paz, B.C.S. 23096, Mexico; Department of Entomology and Plant Pathology, 
      Auburn University, 301 Funchess Hall, Auburn, AL 36849, USA.
FAU - Bashan, Yoav
AU  - Bashan Y
AD  - The Bashan Institute of Science, 1730 Post Oak Ct., AL 36830, USA; Environmental 
      Microbiology Group, Northwestern Center for Biological Research (CIBNOR), Av. IPN 
      195, La Paz, B.C.S. 23096, Mexico; Department of Entomology and Plant Pathology, 
      Auburn University, 301 Funchess Hall, Auburn, AL 36849, USA. Electronic address: 
      bashan@bashanfoundation.org.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160602
PL  - Netherlands
TA  - J Microbiol Methods
JT  - Journal of microbiological methods
JID - 8306883
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Bacillus/classification/*genetics
MH  - Bacillus subtilis/*classification/*genetics/isolation & purification
MH  - Base Sequence
MH  - In Situ Hybridization, Fluorescence
MH  - RNA, Ribosomal, 16S/*genetics
MH  - Rhizosphere
MH  - *Soil Microbiology
OTO - NOTNLM
OT  - B. amyloliquefaciens
OT  - Bacillus subtilis
OT  - Fluorescence in situ hybridization
OT  - Specific probe
EDAT- 2016/06/07 06:00
MHDA- 2018/02/07 06:00
CRDT- 2016/06/07 06:00
PHST- 2016/04/13 00:00 [received]
PHST- 2016/05/30 00:00 [revised]
PHST- 2016/05/31 00:00 [accepted]
PHST- 2016/06/07 06:00 [entrez]
PHST- 2016/06/07 06:00 [pubmed]
PHST- 2018/02/07 06:00 [medline]
AID - S0167-7012(16)30126-9 [pii]
AID - 10.1016/j.mimet.2016.05.029 [doi]
PST - ppublish
SO  - J Microbiol Methods. 2016 Sep;128:125-129. doi: 10.1016/j.mimet.2016.05.029. Epub 
      2016 Jun 2.