PMID- 27264124
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160829
LR  - 20160606
IS  - 1300-7777 (Print)
IS  - 1300-7777 (Linking)
VI  - 28
IP  - 2
DP  - 2011 Jun 5
TI  - Fluorescence in situ hybridization analysis of the hTERC region in acute myeloid 
      leukemia patients.
PG  - 103-6
LID - 10.5152/tjh.2011.25 [doi]
AB  - OBJECTIVE: The telomerase RNA component (hTERC) gene is located at 3q26. 
      Increased hTERC gene expression has been frequently observed and amplification 
      was shown using fluorescence in situ hybridization (FISH) in different cancers. 
      The aim of this study was to determine whether hTERC gene amplification is 
      detectable by FISH in acute myeloid leukemia (AML) cells. METHODS: FISH and 
      karyotype results at the time of diagnosis of 23 adult AML patients were 
      retrospectively evaluated. Additionally, fixed cells were hybridized with an 
      hTERC region-specific FISH probe to determine gene amplification. RESULTS: Ten of 
      the 23 patients had a normal karyotype and 6 had an abnormal karyotype. hTERC 
      region amplification was not observed in any of the patients. CONCLUSION: 
      Although it was reported that hTERC gene amplification may partially contribute 
      to increased telomerase expression and activity in leukemic cells, it is not 
      possible to make such a conclusion based on the results of the this study, as 
      hTERC amplification was not observed in the study group. This suggests that 
      increased telomerase activity via gene amplification in the development of AML 
      may not be as important a factor as it is in solid tumors.
FAU - Ozer, Ozge
AU  - Ozer O
FAU - Bulakbasi Balci, Tugce
AU  - Bulakbasi Balci T
FAU - Yilmaz, Zerrin
AU  - Yilmaz Z
FAU - Sahin, Feride Iffet
AU  - Sahin FI
AD  - Department of Medical Genetics, Faculty of Medicine, Baskent University, Kubilay 
      Sok. No: 36 06570 Maltepe, Ankara, Turkey Phone: +90 312 232 44 00-302 E-mail: 
      feridesahin@hotmail.com.
LA  - eng
PT  - Journal Article
TT  - Akut myeloid losemi olgularinda floresan in situ hibridizasyon yontemi ile hTERC 
      bolgesinin analizi.
PL  - Turkey
TA  - Turk J Haematol
JT  - Turkish journal of haematology : official journal of Turkish Society of 
      Haematology
JID - 9606065
EDAT- 2011/06/05 00:00
MHDA- 2011/06/05 00:01
CRDT- 2016/06/07 06:00
PHST- 2016/06/07 06:00 [entrez]
PHST- 2011/06/05 00:00 [pubmed]
PHST- 2011/06/05 00:01 [medline]
AID - 10.5152/tjh.2011.25 [doi]
PST - ppublish
SO  - Turk J Haematol. 2011 Jun 5;28(2):103-6. doi: 10.5152/tjh.2011.25.