PMID- 27264648
OWN - NLM
STAT- MEDLINE
DCOM- 20170306
LR  - 20170306
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 91
DP  - 2016 Oct
TI  - Rutin-encapsulated chitosan nanoparticles targeted to the brain in the treatment 
      of Cerebral Ischemia.
PG  - 640-55
LID - S0141-8130(16)30527-X [pii]
LID - 10.1016/j.ijbiomac.2016.06.001 [doi]
AB  - OBJECTIVE: Rutin, a potent antioxidant, has been reported to reduce the risk of 
      ischemic disease. Our study aims to prepare rutin-encapsulated-chitosan 
      nanoparticles (RUT-CS-NPs) via ionic gelation method and determine its results, 
      based on different parameters i.e. surface morphology characterization, in-vitro 
      or ex-vivo release, dynamic light scattering and differential scanning 
      calorimetry (DSC), for treating cerebral ischemia. METHODS: UPLC-ESI-Q-TOF-MS/MS 
      was used to evaluate the optimized RT-CS-NPs1 for brain-drug uptake as well as to 
      follow-up the pharmacokinetics, bio-distrbution, brain-targeting efficiency and 
      potential after intranasal administration (i.n.). KEY FINDINGS: A particle size 
      of <100nm for the formulation, significantly affected by drug:CS ratio, and 
      entrapment efficiency and loading capacity of 84.98%+/-4.18% and 39.48%+/-3.16%, 
      respectively were observed for RUT. Pharmacokinetics, bio-distribution, 
      brain-targeting efficiency (1443.48+/-39.39%) and brain drug-targeting potential 
      (93.00+/-5.69%) showed enhanced bioavailability for RUT in brain as compared to 
      intravenous administration. In addition; improved neurobehavioral activity, 
      histopathology and reduced infarction volume effects were observed in middle 
      cerebral artery occlusion (MCAO) induced cerebral ischemic rats model after i.n. 
      administration of RUT-CS-NPs. CONCLUSION: A significant role of 
      mucoadhesive-RT-CS-NPs1 as observed after high targeting potential and efficiency 
      of the formulation prove; RUT-CS-NPs are more effectively accessed and target 
      easily the brain.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Ahmad, Niyaz
AU  - Ahmad N
AD  - Department of Pharmaceutics, College of Clinical Pharmacy, Dammam University, 
      Dammam 31441, Saudi Arabia. Electronic address: nanhussain@uod.edu.sa.
FAU - Ahmad, Rizwan
AU  - Ahmad R
AD  - Department of Natural Products and Alternative Medicine, College of Clinical 
      Pharmacy, Dammam University, Dammam 31441, Saudi Arabia.
FAU - Naqvi, Atta Abbas
AU  - Naqvi AA
AD  - Department of Pharmacy Practice, College of Clinical Pharmacy, University of 
      Dammam, Dammam 31441, Saudi Arabia.
FAU - Alam, Md Aftab
AU  - Alam MA
AD  - Department of Pharmaceutics, School of Medical and Allied Sciences, Galgotias 
      University, Gautam Budh Nagar, Greater Noida 201310, India.
FAU - Ashafaq, Mohammad
AU  - Ashafaq M
AD  - Neuroscience and Toxicology Unit, College of Pharmacy, Jazan University, Jazan, 
      Saudi Arabia.
FAU - Samim, Mohd
AU  - Samim M
AD  - Department of Chemistry, Faculty of Science, Hamdard University, New Delhi 
      110062, India.
FAU - Iqbal, Zeenat
AU  - Iqbal Z
AD  - Nanomedicine Lab, Department of Pharmaceutics, Faculty of Pharmacy, Jamia 
      Hamdard, Hamdard Nagar, New Delhi 110062, India.
FAU - Ahmad, Farhan Jalees
AU  - Ahmad FJ
AD  - Nanomedicine Lab, Department of Pharmaceutics, Faculty of Pharmacy, Jamia 
      Hamdard, Hamdard Nagar, New Delhi 110062, India.
LA  - eng
PT  - Journal Article
DEP - 20160602
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Placebos)
RN  - 0 (Polymers)
RN  - 5G06TVY3R7 (Rutin)
RN  - 9012-76-4 (Chitosan)
SB  - IM
MH  - Adhesiveness
MH  - Animals
MH  - Biological Transport/drug effects
MH  - Brain/drug effects/*pathology
MH  - Brain Ischemia/*drug therapy/pathology
MH  - Calorimetry, Differential Scanning
MH  - Chitosan/*chemistry
MH  - Chromatography, High Pressure Liquid
MH  - *Drug Delivery Systems
MH  - Dynamic Light Scattering
MH  - Goats
MH  - Hand Strength
MH  - Nanoparticles/*chemistry/ultrastructure
MH  - Nasal Mucosa/drug effects
MH  - Particle Size
MH  - Permeability/drug effects
MH  - Placebos
MH  - Polymers/chemistry
MH  - Rats, Wistar
MH  - Reproducibility of Results
MH  - Rutin/pharmacokinetics/pharmacology/*therapeutic use
MH  - Spectrometry, Mass, Electrospray Ionization
MH  - Tandem Mass Spectrometry
MH  - Tissue Distribution/drug effects
OTO - NOTNLM
OT  - Brain pharmacokinetics and pharmacodynamics
OT  - Cerebral ischemia
OT  - Chitosan nanoparticles
OT  - Rutin
OT  - UPLC-MS/MS method validation
EDAT- 2016/06/07 06:00
MHDA- 2017/03/07 06:00
CRDT- 2016/06/07 06:00
PHST- 2016/05/10 00:00 [received]
PHST- 2016/06/01 00:00 [revised]
PHST- 2016/06/01 00:00 [accepted]
PHST- 2016/06/07 06:00 [entrez]
PHST- 2016/06/07 06:00 [pubmed]
PHST- 2017/03/07 06:00 [medline]
AID - S0141-8130(16)30527-X [pii]
AID - 10.1016/j.ijbiomac.2016.06.001 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2016 Oct;91:640-55. doi: 10.1016/j.ijbiomac.2016.06.001. 
      Epub 2016 Jun 2.