PMID- 27265209
OWN - NLM
STAT- MEDLINE
DCOM- 20180424
LR  - 20191210
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 6
DP  - 2016 Jun 6
TI  - Triggering through NOD-2 Differentiates Bone Marrow Precursors to Dendritic Cells 
      with Potent Bactericidal activity.
PG  - 27263
LID - 10.1038/srep27263 [doi]
LID - 27263
AB  - Dendritic cells (DCs) play a crucial role in bridging innate and adaptive 
      immunity by activating naive T cells. The role of pattern recognition receptors 
      like Toll-Like Receptors and Nod-Like Receptors expressed on DCs is well-defined 
      in the recognition of the pathogens. However, nothing is precisely studied 
      regarding the impact of NOD-2 signaling during the differentiation of DCs. 
      Consequently, we explored the role of NOD-2 signaling in the differentiation of 
      DCs and therefore their capability to activate innate and adaptive immunity. 
      Intriguingly, we observed that NOD-2 stimulated DCs (nDCs) acquired highly 
      activated and matured phenotype and exhibited substantially greater bactericidal 
      activity by robust production of nitric oxide. The mechanism involved in 
      improving the functionality of nDCs was dependent on IFN-alphabeta signaling, leading to 
      the activation of STAT pathways. Furthermore, we also observed that STAT-1 and 
      STAT-4 dependent maturation and activation of DCs was under the feedback 
      mechanism of SOCS-1 and SOCS-3 proteins. nDCs acquired enhanced potential to 
      activate chiefly Th1 and Th17 immunity. Taken together, these results suggest 
      that nDCs can be exploited as an immunotherapeutic agent in bolstering host 
      immunity and imparting protection against the pathogens.
FAU - Khan, Nargis
AU  - Khan N
AD  - CSIR-Institute of Microbial Technology, Chandigarh, 160036, INDIA.
FAU - Aqdas, Mohammad
AU  - Aqdas M
AD  - CSIR-Institute of Microbial Technology, Chandigarh, 160036, INDIA.
FAU - Vidyarthi, Aurobind
AU  - Vidyarthi A
AD  - CSIR-Institute of Microbial Technology, Chandigarh, 160036, INDIA.
FAU - Negi, Shikha
AU  - Negi S
AD  - CSIR-Institute of Microbial Technology, Chandigarh, 160036, INDIA.
FAU - Pahari, Susanta
AU  - Pahari S
AD  - CSIR-Institute of Microbial Technology, Chandigarh, 160036, INDIA.
FAU - Agnihotri, Tapan
AU  - Agnihotri T
AD  - CSIR-Institute of Microbial Technology, Chandigarh, 160036, INDIA.
FAU - Agrewala, Javed N
AU  - Agrewala JN
AD  - CSIR-Institute of Microbial Technology, Chandigarh, 160036, INDIA.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160606
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Interferon-alpha)
RN  - 0 (Nod2 Signaling Adaptor Protein)
RN  - 0 (Nod2 protein, mouse)
RN  - 0 (STAT Transcription Factors)
RN  - 77238-31-4 (Interferon-beta)
SB  - IM
MH  - Adaptive Immunity
MH  - Animals
MH  - Bone Marrow Cells/*cytology/*immunology/metabolism/microbiology
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Dendritic Cells/*cytology/*immunology/metabolism/microbiology
MH  - Immunity, Innate
MH  - Interferon-alpha/metabolism
MH  - Interferon-beta/metabolism
MH  - Mice
MH  - Mycobacterium/growth & development
MH  - Nod2 Signaling Adaptor Protein/*metabolism
MH  - STAT Transcription Factors/metabolism
MH  - Signal Transduction
MH  - Th1 Cells/immunology/metabolism
MH  - Th17 Cells/immunology/metabolism
PMC - PMC4893688
EDAT- 2016/06/07 06:00
MHDA- 2018/04/25 06:00
PMCR- 2016/06/06
CRDT- 2016/06/07 06:00
PHST- 2016/03/09 00:00 [received]
PHST- 2016/05/12 00:00 [accepted]
PHST- 2016/06/07 06:00 [entrez]
PHST- 2016/06/07 06:00 [pubmed]
PHST- 2018/04/25 06:00 [medline]
PHST- 2016/06/06 00:00 [pmc-release]
AID - srep27263 [pii]
AID - 10.1038/srep27263 [doi]
PST - epublish
SO  - Sci Rep. 2016 Jun 6;6:27263. doi: 10.1038/srep27263.