PMID- 27265456 OWN - NLM STAT- MEDLINE DCOM- 20171102 LR - 20171204 IS - 1873-2518 (Electronic) IS - 0264-410X (Linking) VI - 34 IP - 33 DP - 2016 Jul 19 TI - Probing vaccine antigens against bovine mastitis caused by Streptococcus uberis. PG - 3848-54 LID - S0264-410X(16)30370-X [pii] LID - 10.1016/j.vaccine.2016.05.044 [doi] AB - Streptococcus uberis is a worldwide pathogen that causes intramammary infections in dairy cattle. Because virulence factors determining the pathogenicity of S. uberis have not been clearly identified so far, a commercial vaccine is not yet available. Different S. uberis strains have the ability to form biofilm in vitro, although the association of this kind of growth with the development of mastitis is unknown. The objective of this study was to evaluate the potential use as vaccine antigens of proteins from S. uberis biofilms, previously identified by proteomic and immunological analyses. The capability of eliciting a protective immune response by targeted candidates was assayed on a murine model. Sera from rabbits immunized with S. uberis biofilm preparations and a convalescent cow intra-mammary infected with S. uberis were probed against cell wall proteins from biofilm and planktonic cells previously separated by two-dimensional gel electrophoresis. Using rabbit immunized serum, two proteins were found to be up-regulated in biofilm cells as compared to planktonic cells; when serum from the convalescent cow was used, up to sixteen biofilm proteins were detected. From these proteins, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), fructose-biphosphate aldolase (FBA), and elongation factor Ts (EFTs) were chosen to be tested as vaccine antigen candidates. For this purpose, different groups of mice were immunized with the three recombinant-expressed proteins (each one formulated separately in a vaccine), and thereafter intraperitoneally challenged with S. uberis. The three proteins induced specific IgG antibodies, but a significant reduction of mortality was only observed in the groups of mice vaccinated with FBA or EFTs. These results suggest that FBA and EFTs might be considered as strong antigenic candidates for a vaccine against S. uberis bovine mastitis. Moreover, this is the first study to indicate that also in S. uberis, GAPDH, FBA and EFTs, as proteins detected in both cytoplasm and cell wall fractions, can play a second function (moonlighting), the latter being particularly involved in the virulence of such a pathogen organism. CI - Copyright (c) 2016 Elsevier Ltd. All rights reserved. FAU - Collado, Rosa AU - Collado R AD - Hipra Scientific, S.L.U., 17170 Amer, Girona, Spain. FAU - Prenafeta, Antoni AU - Prenafeta A AD - Hipra Scientific, S.L.U., 17170 Amer, Girona, Spain. Electronic address: antoni.prenafeta@hipra.com. FAU - Gonzalez-Gonzalez, Luis AU - Gonzalez-Gonzalez L AD - Hipra Scientific, S.L.U., 17170 Amer, Girona, Spain; Departament de Bioquimica i Biologia Molecular, Institut de Biotecnologia i Biomedicina, Universitat Autonoma de Barcelona, Bellaterra, 08193 Barcelona, Spain. FAU - Perez-Pons, Josep Antoni AU - Perez-Pons JA AD - Departament de Bioquimica i Biologia Molecular, Institut de Biotecnologia i Biomedicina, Universitat Autonoma de Barcelona, Bellaterra, 08193 Barcelona, Spain. FAU - Sitja, Marta AU - Sitja M AD - Hipra Scientific, S.L.U., 17170 Amer, Girona, Spain. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20160606 PL - Netherlands TA - Vaccine JT - Vaccine JID - 8406899 RN - 0 (Antibodies, Bacterial) RN - 0 (Antigens, Bacterial) RN - 0 (Bacterial Vaccines) RN - 0 (Immunoglobulin G) RN - 0 (Peptide Elongation Factors) RN - 0 (Recombinant Proteins) RN - 0 (elongation factor Ts) RN - EC 3.1.3.11 (Fructose-Bisphosphatase) SB - IM MH - Animals MH - Antibodies, Bacterial/blood MH - Antigens, Bacterial/*immunology MH - Bacterial Vaccines/*immunology MH - Biofilms MH - Cattle MH - Electrophoresis, Gel, Two-Dimensional MH - Female MH - Fructose-Bisphosphatase/immunology MH - Gene Expression Regulation, Bacterial MH - Immunoglobulin G/blood MH - Mastitis, Bovine/*prevention & control MH - Mice MH - Mice, Inbred BALB C MH - Peptide Elongation Factors/immunology MH - Proteomics MH - Rabbits MH - Recombinant Proteins/immunology MH - Streptococcal Infections/prevention & control/*veterinary MH - *Streptococcus OTO - NOTNLM OT - Mastitis OT - Streptococcus uberis OT - Vaccine EDAT- 2016/06/07 06:00 MHDA- 2017/11/03 06:00 CRDT- 2016/06/07 06:00 PHST- 2016/03/06 00:00 [received] PHST- 2016/05/03 00:00 [revised] PHST- 2016/05/20 00:00 [accepted] PHST- 2016/06/07 06:00 [entrez] PHST- 2016/06/07 06:00 [pubmed] PHST- 2017/11/03 06:00 [medline] AID - S0264-410X(16)30370-X [pii] AID - 10.1016/j.vaccine.2016.05.044 [doi] PST - ppublish SO - Vaccine. 2016 Jul 19;34(33):3848-54. doi: 10.1016/j.vaccine.2016.05.044. Epub 2016 Jun 6.