PMID- 27268206
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20200326
IS  - 1746-6148 (Electronic)
IS  - 1746-6148 (Linking)
VI  - 12
DP  - 2016 Jun 6
TI  - Nonstructural protein 11 (nsp11) of porcine reproductive and respiratory syndrome 
      virus (PRRSV) promotes PRRSV infection in MARC-145 cells.
PG  - 90
LID - 10.1186/s12917-016-0717-5 [doi]
LID - 90
AB  - BACKGROUND: Porcine reproductive and respiratory syndrome virus (PRRSV) induces 
      one of most important devastating disease of swine worldwide, and the current 
      methods poorly control it. Previous studies have indicated that the nonstructural 
      protein 11 (nsp11) of PRRSV may be an important protein for the immune escape of 
      PRRSV. RESULTS: Here, we firstly explored the effect of over-expression of nsp11 
      on PRRSV infection and found that over-expression of nsp11 enhanced the PRRSV 
      titers while the small interfering RNA (siRNAs) specifically targeting nsp11 
      could reduce the PRRSV titers in MARC-145 cells. CONCLUSION: In conclusion, PRRSV 
      nsp11 promotes PRRSV infection in MARC-145 cells and siRNAs targeting nsp11 may 
      be a potential therapeutic strategy to control PRRSV in future.
FAU - Shi, Xibao
AU  - Shi X
AD  - College of Life Sciences, Henan Normal University, Xinxiang, 453007, China. 
      shixibao@aliyun.com.
AD  - Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan 
      Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural 
      Sciences, Zhengzhou, Henan, 450002, China. shixibao@aliyun.com.
FAU - Zhang, Xiaozhuan
AU  - Zhang X
AD  - College of Life Sciences, Henan Normal University, Xinxiang, 453007, China.
AD  - College of Veterinary Medicine and Animal Science, Henan Agricultural University, 
      Zhengzhou, Henan, 450002, China.
FAU - Chang, Yongzhe
AU  - Chang Y
AD  - Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan 
      Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural 
      Sciences, Zhengzhou, Henan, 450002, China.
FAU - Jiang, Bo
AU  - Jiang B
AD  - Office of Science & Technology, Chongqing Police College, Chongqing, 401331, 
      China.
FAU - Deng, Ruiguang
AU  - Deng R
AD  - Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan 
      Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural 
      Sciences, Zhengzhou, Henan, 450002, China.
FAU - Wang, Aiping
AU  - Wang A
AD  - Department of Bioengineering, Zhengzhou University, Zhengzhou, Henan, 450000, 
      450002, China.
FAU - Zhang, Gaiping
AU  - Zhang G
AD  - Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan 
      Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural 
      Sciences, Zhengzhou, Henan, 450002, China. zhanggaiping2003@163.com.
AD  - College of Veterinary Medicine and Animal Science, Henan Agricultural University, 
      Zhengzhou, Henan, 450002, China. zhanggaiping2003@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160606
PL  - England
TA  - BMC Vet Res
JT  - BMC veterinary research
JID - 101249759
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Viral Nonstructural Proteins)
RN  - EC 3.1.- (Endoribonucleases)
RN  - EC 3.1.- (nidoviral uridylate-specific endoribonuclease)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Endoribonucleases/metabolism
MH  - Gene Expression
MH  - Gene Targeting
MH  - HEK293 Cells
MH  - Humans
MH  - Porcine Reproductive and Respiratory Syndrome/therapy/virology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*physiology
MH  - RNA, Small Interfering
MH  - Swine
MH  - Transfection
MH  - Viral Nonstructural Proteins/genetics/*physiology
MH  - Virus Replication
PMC - PMC4895886
OTO - NOTNLM
OT  - PRRSV
OT  - Small interfering RNA
OT  - nsp11
EDAT- 2016/06/09 06:00
MHDA- 2016/12/15 06:00
PMCR- 2016/06/06
CRDT- 2016/06/09 06:00
PHST- 2015/11/16 00:00 [received]
PHST- 2016/06/01 00:00 [accepted]
PHST- 2016/06/09 06:00 [entrez]
PHST- 2016/06/09 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
PHST- 2016/06/06 00:00 [pmc-release]
AID - 10.1186/s12917-016-0717-5 [pii]
AID - 717 [pii]
AID - 10.1186/s12917-016-0717-5 [doi]
PST - epublish
SO  - BMC Vet Res. 2016 Jun 6;12:90. doi: 10.1186/s12917-016-0717-5.