PMID- 27268319
OWN - NLM
STAT- MEDLINE
DCOM- 20170324
LR  - 20170324
IS  - 1537-6524 (Electronic)
IS  - 1537-6516 (Linking)
VI  - 26
IP  - 5
DP  - 2016 Jun
TI  - Thirdhand tobacco smoke: procedures to evaluate cytotoxicity in cell cultures.
PG  - 355-61
LID - 10.1080/15376516.2016.1188190 [doi]
AB  - The risks associated to tobacco smoking are not ceased with smoke extinction. 
      Many toxic compounds remain in the environment after the cigarette is 
      extinguished and accumulated in the air or on surfaces. However, little is known 
      about the risks of this exposure. The aim of this study was to evaluate 
      procedures to collect thirdhand smoke (THS) and prepare the samples to perform 
      three in vitro toxicity tests. Cellulose papers and cotton wipes were used to 
      impregnate with nicotine solution and smoke cigarette in a chamber or in smoker's 
      home. Samples were immersed in methanol or Dulbecco's modified Eagle's medium 
      (DMEM) to expose Hep-2 cells. MTT, neutral red uptake (NRU) and trypan blue 
      assays were performed. The concentration of nicotine in DMEM extract of THS in 
      paper and cotton was similar to those in methanol extract (p > 0.05). Alterations 
      in the mitochondrial and lysosomal functions were found in both paper and cotton 
      samples; however, the cytotoxic effect was not always observed. There was a 
      decrease of 21-31% in MTT assay and 38-56% in NRU assay (p < 0.003). There was a 
      dose-response relationship between the amount of cigarettes and lysosomal 
      viability; the correlation was higher for cotton samples (r = -0.843, p < 0.001). 
      As a dose-response relationship was found only in NRU assay, this test may be a 
      more suitable choice rather than the MTT assay. Paper and wipe sampling can be 
      reliable markers of tobacco smoke contamination. Moreover, these materials, if 
      properly prepared, can be used as substrate providers to perform cellular assays.
FAU - Figueiro, Luciana Rizzieri
AU  - Figueiro LR
AD  - a Graduate Program in Pathology , Federal University of Health Sciences of Porto 
      Alegre (UFCSPA) , Porto Alegre , RS , Brazil ;
FAU - Dantas, Denise Conceicao Mesquita
AU  - Dantas DC
AD  - b Pharmacoscience Department , UFCSPA , Porto Alegre , RS , Brazil ;
FAU - Linden, Rafael
AU  - Linden R
AD  - c Toxicology Laboratory , Institute of Health Sciences, Feevale University , Novo 
      Hamburgo , RS , Brazil ;
FAU - Ziulkoski, Ana Luiza
AU  - Ziulkoski AL
AD  - d Cytotoxicity Laboratory , Institute of Health Sciences, Feevale University , 
      Novo Hamburgo , RS , Brazil.
LA  - eng
PT  - Journal Article
DEP - 20160607
PL  - England
TA  - Toxicol Mech Methods
JT  - Toxicology mechanisms and methods
JID - 101134521
RN  - 0 (Air Pollutants)
RN  - 0 (Tobacco Smoke Pollution)
RN  - 6M3C89ZY6R (Nicotine)
SB  - IM
MH  - Air Pollutants/*toxicity
MH  - Air Pollution, Indoor/*adverse effects/analysis
MH  - Biological Assay
MH  - Cell Culture Techniques
MH  - Cell Proliferation/*drug effects
MH  - Cell Survival/drug effects
MH  - Environmental Monitoring/*methods
MH  - Hep G2 Cells
MH  - Humans
MH  - Nicotine/analysis
MH  - Tobacco Smoke Pollution/*adverse effects/analysis
OTO - NOTNLM
OT  - Environmental tobacco smoke
OT  - MTT assay
OT  - neutral red uptake assay
OT  - nicotine
OT  - trypan blue assay
EDAT- 2016/06/09 06:00
MHDA- 2017/03/25 06:00
CRDT- 2016/06/09 06:00
PHST- 2016/06/09 06:00 [entrez]
PHST- 2016/06/09 06:00 [pubmed]
PHST- 2017/03/25 06:00 [medline]
AID - 10.1080/15376516.2016.1188190 [doi]
PST - ppublish
SO  - Toxicol Mech Methods. 2016 Jun;26(5):355-61. doi: 10.1080/15376516.2016.1188190. 
      Epub 2016 Jun 7.